Assessment of adenyl residue reactivity within model nucleic acids by surface enhanced Raman spectroscopy

Biopolymers ◽  
2006 ◽  
Vol 82 (1) ◽  
pp. 6-28 ◽  
Author(s):  
Lydie Grajcar ◽  
Chahrazade El Amri ◽  
Mahmoud Ghomi ◽  
Serge Fermandjian ◽  
Valerie Huteau ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Nucleic Acids ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Structural Recognition of Triple-Stranded DNA by Surface-Enhanced Raman Spectroscopy

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 326
Author(s):  
Luca Guerrini ◽  
Ramon A. Alvarez-Puebla
Keyword(s):  
Raman Spectroscopy ◽  
Nucleic Acids ◽  
Double Helix ◽  
Surface Enhanced Raman Spectroscopy ◽  
Label Free ◽  
Helix Formation ◽  
Phosphate Backbone ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Triplex Structure

Direct, label-free analysis of nucleic acids via surface-enhanced Raman spectroscopy (SERS) has been continuously expanding its range of applications as an intriguing and powerful analytical tool for the structural characterization of diverse DNA structures. Still, interrogation of nucleic acid tertiary structures beyond the canonical double helix often remains challenging. In this work, we report for the first time the structural identification of DNA triplex structures. This class of nucleic acids has been attracting great interest because of their intriguing biological functions and pharmacological potential in gene therapy, and the ability for precisely engineering DNA-based functional nanomaterials. Herein, structural discrimination of the triplex structure against its duplex and tertiary strand counterparts is univocally revealed by recognizing key markers bands in the intrinsic SERS fingerprint. These vibrational features are informative of the base stacking, Hoogsteen hydrogen bonding and sugar–phosphate backbone reorganization associated with the triple helix formation. This work expands the applicability of direct SERS to nucleic acids analysis, with potential impact on fields such as sensing, biology and drug design.

scholarly journals Targets and Tools: Nucleic Acids for Surface-Enhanced Raman Spectroscopy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 230
Author(s):  
Irene Calderon ◽  
Luca Guerrini ◽  
Ramon A. Alvarez-Puebla
Keyword(s):  
Raman Spectroscopy ◽  
Nucleic Acids ◽  
Dna Nanotechnology ◽  
Surface Enhanced Raman Spectroscopy ◽  
Optical Signal ◽  
Analytical Tool ◽  
Feature Article ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Structural Dna Nanotechnology

Surface-enhanced Raman spectroscopy (SERS) merges nanotechnology with conventional Raman spectroscopy to produce an ultrasensitive and highly specific analytical tool that has been exploited as the optical signal read-out in a variety of advanced applications. In this feature article, we delineate the main features of the intertwined relationship between SERS and nucleic acids (NAs). In particular, we report representative examples of the implementation of SERS in biosensing platforms for NA detection, the integration of DNA as the biorecognition element onto plasmonic materials for SERS analysis of different classes of analytes (from metal ions to microorgniasms) and, finally, the use of structural DNA nanotechnology for the precise engineering of SERS-active nanomaterials.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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