Backbone dynamics of human parathyroid hormone (1-34): Flexibility of the central region under different environmental conditions

Biopolymers ◽  
2006 ◽  
Vol 84 (2) ◽  
pp. 147-160 ◽  
Author(s):  
Michele Scian ◽  
Massimiliano Marin ◽  
Massimo Bellanda ◽  
Liqiang Tou ◽  
Joseph M. Alexander ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Central Region ◽  
Environmental Conditions ◽  
Backbone Dynamics ◽  
Human Parathyroid Hormone

A radioimmunoassay for human parathyroid hormone utilizing a goat anti-bovine PTH serum

1981 ◽  
Vol 96 (2) ◽  
pp. 215-221 ◽  
Author(s):  
L. E. Mallette
Keyword(s):  
Parathyroid Hormone ◽  
Primary Hyperparathyroidism ◽  
Secondary Hyperparathyroidism ◽  
Human Serum ◽  
Central Region ◽  
Antigenic Site ◽  
Human Parathyroid Hormone ◽  
Domestic Goat ◽  
Standard Serum ◽  
Dilution Curves

Abstract. An antiserum (NG-1) against bovine PTH (bPTH) generated in the domestic goat was characterized for use in the radioimmunoassay of PTH in human serum. When a carboxyterminal fragment of bPTH is used as radioligand, this antiserum detects only an antigenic site in the central region of the hPTH molecule. The synthetic hormone fragment, hPTH-(44-68), will displace 93% of the tracer, after which the addition of intact hPTH causes no further displacement. The assay does not detect the synthetic aminoterminal 1-34 fragment of the bovine or human hormones, nor the carboxyterminal fragment of the human hormone, hPTh-(53-84). Standard curves with bPTH-(1-84) and partially purified hPTH are not parallel, so that hPTH is used as standard. Serum from subjects with uraemia or primary hyperparathyroidism gives dilution curves parallel to that with the hPTH standard. The assay with NG-1 has been applied to the diagnosis of primary and secondary hyperparathyroidism, used to monitor the disappearance of PTH after parathyroidectomy, and for measurement of PTH in selective venous samples.

75SELEN-LABELED HUMAN PARATHYROID HORMONE FROM TISSUE CULTURES

1973 ◽  
Vol 71 (4_Suppl) ◽  
pp. S163
Author(s):  
R. Hehrmann ◽  
E. Jentsch ◽  
H. Mayer ◽  
R. Montz
Keyword(s):  
Parathyroid Hormone ◽  
Tissue Cultures ◽  
Human Parathyroid Hormone

scholarly journals Purification and Characterization of Recombinant Human Parathyroid Hormone-related Protein

1989 ◽  
Vol 264 (25) ◽  
pp. 14806-14811
Author(s):  
R G Hammonds ◽  
P McKay ◽  
G A Winslow ◽  
H Diefenbach-Jagger ◽  
V Grill ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Related Protein ◽  
Human Parathyroid Hormone ◽  
Purification And Characterization ◽  
Parathyroid Hormone Related Protein
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