Atomic force microscopy of a hybrid high-molecular-weight glutenin subunit from a transgenic hexaploid wheat

Biopolymers ◽  
2005 ◽  
Vol 78 (2) ◽  
pp. 53-61 ◽  
Author(s):  
Theresa M. McIntire ◽  
Ellen J. L. Lew ◽  
A. Elva Adalsteins ◽  
Ann Blechl ◽  
Olin D. Anderson ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Atomic Force Microscopy ◽  
High Molecular Weight ◽  
Hexaploid Wheat ◽  
Glutenin Subunit ◽  
Force Microscopy ◽  
Atomic Force

Atomic Force Microscopy Contact Mode Study on Ultra High Molecular Weight Polyethylene

2005 ◽  
Vol 874 ◽  
Author(s):  
Yifang Cao ◽  
Jikou Zhou ◽  
Oludele Popoola ◽  
Dal F. Swarts ◽  
Wole Soboyejo
Keyword(s):  
Experimental Data ◽  
Molecular Weight ◽  
Atomic Force Microscopy ◽  
Young’S Modulus ◽  
High Molecular Weight ◽  
Work Of Adhesion ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Ultra High Molecular Weight

AbstractThis paper presents the results of contact mode atomic force microscopy (AFM) study on the nanoscale Young's modulus and work of adhesion of ultra high molecular weight polyethylene (UHMWPE). Cryoultramicrotomed surfaces of UHMWPE were scanned using the contact mode of AFM. Fibril regions are commonly found on the sample, however, a non-fibril particulate region was also found. AFM force displacement curves were obtained for the sample. The JKR theory and Maugis Dugdale model were used for the analysis. A good fitting between the theories and experimental data was found. The nanoscale Young's modulus and work of adhesion of UHMWPE extracted from the experimental data were in reasonably good agreement with the values reported in other literatures.

Stereocomplex electrospun fibers from high molecular weight of poly(L-lactic acid) and poly(D-lactic acid)

2020 ◽  
Vol 40 (2) ◽  
pp. 136-142 ◽  
Author(s):  
Homa Maleki ◽  
Hossein Barani
Keyword(s):  
Molecular Weight ◽  
Lactic Acid ◽  
Thermal Properties ◽  
High Molecular Weight ◽  
Chemical Properties ◽  
Electrospun Fibers ◽  
Scanning Calorimetry ◽  
Force Microscopy ◽  
Atomic Force ◽  
Electrospinning Method

AbstractThe stereocomplex formation is a promising method to improve the properties of poly(lactide) (PLA)-based products due to the strong interaction of the side-by-side arrangement of the molecular chains. Recently, electrospinning method has been applied to prepare PLA stereocomplex, which is more convenient. The objective of the current study is to make stereocomplexed PLA nanofibers using electrospinning method and compare their properties and structures with pure poly(l-lactide) (PLLA) fibers. The stereocomplexed fibers were electrospun from a blend solution of high molecular weight PLLA and poly(d-lactide) (1:1 ratio). The morphology of the obtained electrospun fibers was examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Differential scanning calorimetry was applied to study their thermal properties and crystallinity. Fourier transform infrared spectroscopy (FTIR) test was conducted on the samples to characterize their chemical properties. The SEM and AFM images indicated that smooth uniform fibers with a cylindrical structure were produced. Besides, the FTIR results and thermal properties confirmed that only stereocomplex crystallites formed in the resulting fibers via the electrospinning method.

The association between high molecular weight glutenin subunit compositions and the bread-making quality of Chinese and Japanese hexaploid wheats

2000 ◽  
Vol 51 (3) ◽  
pp. 371 ◽  
Author(s):  
H. Nakamura
Keyword(s):  
Molecular Weight ◽  
Genetic Variation ◽  
High Molecular Weight ◽  
Hexaploid Wheat ◽  
Allelic Variation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Glutenin Subunit ◽  
Wheat Varieties ◽  
Hmw Glutenin

Variation in the electrophoretic banding patterns of high molecular weight (HMW) glutenin subunits of 274 hexaploid wheat (Triticum aestivum) varieties from China was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Twenty-seven different major glutenin HMW subunits were identified. Each variety contained 3–5 subunits and 29 different glutenin subunit patterns were segregated. Seventeen alleles were identified based on comparison of subunit mobilities with those previously found for hexaploid wheat. Chinese hexaploid wheats exhibited particular allelic variation in glutenin HMW subunit composition and this variation differed from that found in wheats from Japanese and other countries. Average Glu-1 quality scores of 274 Chinese wheat varieties in the present study have been shown to be higher than that of Japanese wheats. Considerable genetic variation in the HMW glutenin subunit compositions of the Chinese wheats was observed in the present study and previously. Alleles from Chinese hexaploid wheat varieties have not been extensively introduced into Japan and other countries. The present data may indicate possible applications of Chinese germplasm in wheat breeding programs. To improve the wheat quality, genetic variation should be attempted through the introduction of genes of Chinese varieties into varieties in Japan and other countries.

The high-molecular-weight glutenin subunit composition of Japanese hexaploid wheat landraces

2000 ◽  
Vol 51 (6) ◽  
pp. 673 ◽  
Author(s):  
H. Nakamura
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Hexaploid Wheat ◽  
Storage Proteins ◽  
Allelic Variation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Glutenin Subunit ◽  
Subunit Composition ◽  
Wheat Landraces

The endosperm storage proteins of 174 Japanese wheat (Triticum aestivum) landraces were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis to determine their high-molecular-weight (HMW) glutenin subunit composition. These are alleles for complex gene loci, Glu-A1, Glu-B1, and Glu-D1, that are present in Japanese hexaploid wheat landraces. These were identified by comparison with the subunit mobility previously found in hexaploid wheat. Twenty-four different, major glutenin HMW subunits were identified. Each landrace contained 3–5 subunits, and 17 different glutenin subunit patterns were observed for 13 alleles in Japanese landraces. Japanese landraces showed specific allelic variation in glutenin HMW subunits, different from those in non-Japanese hexaploid wheats.

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