Pairing of orthogonal chaperones with a cytochrome P450 enhances terpene synthesis in Saccharomyces cerevisiae

2021 ◽  
pp. 2000452
Author(s):  
Jae‐Eung Kim ◽  
So‐Hee Son ◽  
Seung Soo Oh ◽  
Sun Chang Kim ◽  
Ju Young Lee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome P450 ◽  
Terpene Synthesis

Functional evaluation of cytochrome P450 2D6 with Gly42Arg substitution expressed in Saccharomyces cerevisiae

2001 ◽  
Vol 11 (8) ◽  
pp. 709-718 ◽  
Author(s):  
Daisuke Tsuzuki ◽  
Chie Takemi ◽  
Shigeo Yamamoto ◽  
Keietsu Tamagake ◽  
Susumu Imaoka ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cytochrome P450 ◽  
Functional Evaluation ◽  
Cytochrome P450 2D6 ◽  
P450 2D6

scholarly journals Whole-Cell Biotransformation of 1,12-Dodecanedioic Acid from Coconut Milk Factory Wastewater by Recombinant CYP52A17SS Expressing Saccharomyces cerevisiae

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 969
Author(s):  
Phawadee Buathong ◽  
Nassapat Boonvitthya ◽  
Gilles Truan ◽  
Warawut Chulalaksananukul
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Cytochrome P450 ◽  
Chemical Synthesis ◽  
Value Added ◽  
Coconut Milk ◽  
Biological Synthesis ◽  
Cytochrome P450 Enzyme ◽  
Yeast Growth ◽  
Dodecanedioic Acid

Biotransformation of fatty acids from renewable wastewater as feedstock to value-added chemicals is a fascinating commercial opportunity. α,ω-Dicarboxylic acids (DCAs) are building blocks in many industries, such as polymers, cosmetic intermediates, and pharmaceuticals, and can be obtained by chemical synthesis under extreme conditions. However, biological synthesis can replace the traditional chemical synthesis using cytochrome P450 enzymes to oxidize fatty acids to DCAs. Saccharomyces cerevisiae BY(2R)/pYeDP60-CYP52A17SS (BCM), a transgenic strain expressing the galactose-inducible CYP52A17SS cytochrome P450 enzyme, was able to grow in a coconut milk factory wastewater (CCW) medium and produced 12-hydroxydodecanoic acid (HDDA) and 1,12-dodecanedioic acid (DDA). The supplementation of CCW with 10 g/L yeast extract and 20 g/L peptone (YPCCW) markedly increased the yeast growth rate and the yields of 12-HDDA and 1,12-DDA, with the highest levels of approximately 60 and 38 µg/L, respectively, obtained at 30 °C and pH 5. The incubation temperature and medium pH strongly influenced the yeast growth and 1,12-DDA yield, with the highest 1,12-DDA formation at 30 °C and pH 5–5.5. Hence, the S. cerevisiae BCM strain can potentially be used for producing value-added products from CCW.

Sign in / Sign up

Export Citation Format

Share Document