L‐malic acid production from xylose by engineered Saccharomyces cerevisiae

2021 ◽  
pp. 2000431
Author(s):  
Nam Kyu Kang ◽  
Jae Won Lee ◽  
Donald R. Ort ◽  
Yong‐Su Jin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Production ◽  
Engineered Saccharomyces Cerevisiae

Characteristics of the high malic acid production mechanism in Saccharomyces cerevisiae sake yeast strain No. 28

2012 ◽  
Vol 114 (3) ◽  
pp. 281-285 ◽  
Author(s):  
Shunichi Nakayama ◽  
Ken Tabata ◽  
Takahiro Oba ◽  
Kenichi Kusumoto ◽  
Shinji Mitsuiki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Yeast Strain ◽  
Acid Production ◽  
Production Mechanism

L-malic acid production using immobilized saccharomyces cerevisiae

1991 ◽  
Vol 30 (2) ◽  
pp. 217-224 ◽  
Author(s):  
Z. M. B. Figueiredo ◽  
L. B. Carvalho
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Production

Lactic acid production from xylose by engineered Saccharomyces cerevisiae without PDC or ADH deletion

2015 ◽  
Vol 99 (19) ◽  
pp. 8023-8033 ◽  
Author(s):  
Timothy L. Turner ◽  
Guo-Chang Zhang ◽  
Soo Rin Kim ◽  
Vijay Subramaniam ◽  
David Steffen ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Engineered Saccharomyces Cerevisiae

d-Lactic acid production by metabolically engineered Saccharomyces cerevisiae

2006 ◽  
Vol 101 (2) ◽  
pp. 172-177 ◽  
Author(s):  
Nobuhiro Ishida ◽  
Tomiko Suzuki ◽  
Kenro Tokuhiro ◽  
Eiji Nagamori ◽  
Toru Onishi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Engineered Saccharomyces Cerevisiae

Enhancing oleanolic acid production in engineered Saccharomyces cerevisiae

2018 ◽  
Vol 257 ◽  
pp. 339-343 ◽  
Author(s):  
Yujia Zhao ◽  
Jingjing Fan ◽  
Chen Wang ◽  
Xudong Feng ◽  
Chun Li
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Oleanolic Acid ◽  
Acid Production ◽  
Engineered Saccharomyces Cerevisiae

scholarly journals l-Lactic Acid Production Using Engineered Saccharomyces cerevisiae with Improved Organic Acid Tolerance

2021 ◽  
Vol 7 (11) ◽  
pp. 928
Author(s):  
Byeong-Kwan Jang ◽  
Yebin Ju ◽  
Deokyeol Jeong ◽  
Sung-Keun Jung ◽  
Chang-Kil Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acid Tolerance ◽  
Production Costs ◽  
High Concentration ◽  
Adaptive Laboratory Evolution ◽  
Engineered Saccharomyces Cerevisiae

Lactic acid is mainly used to produce bio-based, bio-degradable polylactic acid. For industrial production of lactic acid, engineered Saccharomyces cerevisiae can be used. To avoid cellular toxicity caused by lactic acid accumulation, pH-neutralizing agents are used, leading to increased production costs. In this study, lactic acid-producing S. cerevisiae BK01 was developed with improved lactic acid tolerance through adaptive laboratory evolution (ALE) on 8% lactic acid. The genetic basis of BK01 could not be determined, suggesting complex mechanisms associated with lactic acid tolerance. However, BK01 had distinctive metabolomic traits clearly separated from the parental strain, and lactic acid production was improved by 17% (from 102 g/L to 119 g/L). To the best of our knowledge, this is the highest lactic acid titer produced by engineered S. cerevisiae without the use of pH neutralizers. Moreover, cellulosic lactic acid production by BK01 was demonstrated using acetate-rich buckwheat husk hydrolysates. Particularly, BK01 revealed improved tolerance against acetic acid of the hydrolysates, a major fermentation inhibitor of lignocellulosic biomass. In short, ALE with a high concentration of lactic acid improved lactic acid production as well as acetic acid tolerance of BK01, suggesting a potential for economically viable cellulosic lactic acid production.

scholarly journals Key Process Conditions for Production of C4 Dicarboxylic Acids in Bioreactor Batch Cultures of an Engineered Saccharomyces cerevisiae Strain

2009 ◽  
Vol 76 (3) ◽  
pp. 744-750 ◽  
Author(s):  
Rintze M. Zelle ◽  
Erik de Hulster ◽  
Wendy Kloezen ◽  
Jack T. Pronk ◽  
Antonius J. A. van Maris
Keyword(s):  
Carbon Dioxide ◽  
Saccharomyces Cerevisiae ◽  
Process Parameters ◽  
Malic Acid ◽  
Acid Production ◽  
Shake Flask ◽  
Simultaneous Optimization ◽  
Process Conditions ◽  
Recent Effort ◽  
Shake Flasks

ABSTRACT A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter−1 of malate at a yield of 0.42 mol (mol glucose)−1 in calcium carbonate-buffered shake flask cultures. With shake flasks, process parameters that are important for scaling up this process cannot be controlled independently. In this study, growth and product formation by the engineered strain were studied in bioreactors in order to separately analyze the effects of pH, calcium, and carbon dioxide and oxygen availability. A near-neutral pH, which in shake flasks was achieved by adding CaCO3, was required for efficient C4 dicarboxylic acid production. Increased calcium concentrations, a side effect of CaCO3 dissolution, had a small positive effect on malate formation. Carbon dioxide enrichment of the sparging gas (up to 15% [vol/vol]) improved production of both malate and succinate. At higher concentrations, succinate titers further increased, reaching 0.29 mol (mol glucose)−1, whereas malate formation strongly decreased. Although fully aerobic conditions could be achieved, it was found that moderate oxygen limitation benefitted malate production. In conclusion, malic acid production with the engineered S. cerevisiae strain could be successfully transferred from shake flasks to 1-liter batch bioreactors by simultaneous optimization of four process parameters (pH and concentrations of CO2, calcium, and O2). Under optimized conditions, a malate yield of 0.48 ± 0.01 mol (mol glucose)−1 was obtained in bioreactors, a 19% increase over yields in shake flask experiments.

Malic acid production and consumption by selected of Saccharomyces cerevisiae under anaerobic and aerobic conditions

1984 ◽  
Vol 19 (6) ◽  
pp. 427-429 ◽  
Author(s):  
Fabrizio Fatichenti ◽  
Giovanni Antonio Farris ◽  
Pietrino Deiana ◽  
Salvatore Ceccarelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Malic Acid ◽  
Acid Production ◽  
Aerobic Conditions ◽  
Production And Consumption

l-Malic acid production from fumaric acid by a laboratory Saccharomyces cerevisiae strain SHY2

1996 ◽  
Vol 18 (12) ◽  
pp. 1441-1446 ◽  
Author(s):  
Xiaohai Wang ◽  
C. S. Gong ◽  
George T. Tsao
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fumaric Acid ◽  
Malic Acid ◽  
Acid Production
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