scholarly journals Engineering Tobacco Mosaic Virus and Its Virus‐Like‐Particles for Synthesis of Biotemplated Nanomaterials

2020 ◽  
pp. 2000311
Author(s):  
Kok Zhi Lee ◽  
Vindula Basnayake Pussepitiyalage ◽  
Yu‐Hsuan Lee ◽  
L. Sue Loesch‐Fries ◽  
Michael T. Harris ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Like Particles

Expanding the genetic code for site-specific labelling of tobacco mosaic virus coat protein and building biotin-functionalized virus-like particles

2014 ◽  
Vol 50 (30) ◽  
pp. 4007-4009 ◽  
Author(s):  
F. C. Wu ◽  
H. Zhang ◽  
Q. Zhou ◽  
M. Wu ◽  
Z. Ballard ◽  
...  
Keyword(s):  
Coat Protein ◽  
Tobacco Mosaic Virus ◽  
Genetic Code ◽  
Mosaic Virus ◽  
Site Specific ◽  
Virus Like Particles ◽  
Specific Modification ◽  
Virus Coat Protein ◽  
Virus Coat ◽  
Specific Labelling

Building biotin-functionalized virus-like particles by combining a genetic code expanding technology and site specific modification of tobacco mosaic virus coat protein.

Interaction between nanocellulose and tobacco mosaic virus-like particles: an atomic force microscopy study

Cellulose ◽  
2020 ◽  
Vol 27 (5) ◽  
pp. 2381-2387 ◽  
Author(s):  
Olga V. Sinitsyna ◽  
Natalia O. Kalinina ◽  
Kara McGeachy ◽  
Eric Whale ◽  
David Hepworth ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Microscopy Study ◽  
Atomic Force Microscopy Study ◽  
Virus Like Particles ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Virus-Like Particle Facilitated Deposition of Hydroxyapatite Bone Mineral on Nanocellulose after Exposure to Phosphate and Calcium Precursors

2019 ◽  
Vol 20 (8) ◽  
pp. 1814 ◽  
Author(s):  
Olga V. Sinitsyna ◽  
Valentine V. Makarov ◽  
Kara McGeachy ◽  
Tatyana Bukharova ◽  
Eric Whale ◽  
...  
Keyword(s):  
Composite Materials ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Bone Mineral ◽  
Calcium Ions ◽  
Negative Charge ◽  
Virus Like Particles ◽  
Virus Like Particle ◽  
Phosphate Salts

We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions. Moreover, we showed that the TMV VLPs could associate strongly with nanocellulose slurry after a simple mixing step. We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs. The TMV VLPs confer mineralization properties to the nanocellulose for the generation of new composite materials.

scholarly journals Engineering Tobacco Mosaic Virus, Barley Stripe Mosaic Virus, and their Virus-Like-Particles for Synthesis of Biotemplated Nanomaterials

2020 ◽  
Author(s):  
Kok Zhi Lee ◽  
Vindula Basnayake Pussepitiya ◽  
Yu Hsuan Lee ◽  
Sue Loesch Fries ◽  
Michael Harris ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Barley Stripe Mosaic Virus ◽  
Virus Like Particles

scholarly journals A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants

Viruses ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 885
Author(s):  
Eva C. Thuenemann ◽  
Matthew J. Byrne ◽  
Hadrien Peyret ◽  
Keith Saunders ◽  
Roger Castells-Graells ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Viral Vector ◽  
Potato Virus X ◽  
Efficient Production ◽  
Coat Proteins ◽  
Rna Sequences ◽  
Virus Like Particles ◽  
Papaya Mosaic Virus

The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-HT). Significantly greater quantities of VLPs could be purified when pEff was used. The pEff system was also very efficient at producing VLPs of helical viruses from different virus families. Examination of the RNA content of AltMV and tobacco mosaic virus VLPs produced from pEff revealed the presence of vector-derived RNA sequences, suggesting that the replicating RNA acts as a scaffold for VLP assembly. Cryo-EM analysis of the AltMV VLPs showed they had a structure very similar to that of authentic potexvirus particles. Thus, we conclude that vectors generating replicating forms of RNA, such as pEff, are very efficient for producing helical VLPs.

Quantitative Measurements on the Ion Etching of TMV

1973 ◽  
Vol 31 ◽  
pp. 336-337
Author(s):  
Irwin Bendet ◽  
Nabil Rizk
Keyword(s):  
Electron Microscope ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Ion Current ◽  
Beam Diameter ◽  
Ion Etching ◽  
Preliminary Results ◽  
Quantitative Measurements ◽  
Monitoring Devices

Preliminary results reported last year on the ion etching of tobacco mosaic virus indicated that the diameter of the virus decreased more rapidly at 10KV than at 5KV, perhaps reaching a constant value before disappearing completely.In order to follow the effects of ion etching on TMV more quantitatively we have designed and built a second apparatus (Fig. 1), which incorporates monitoring devices for measuring ion current and vacuum as well as accelerating voltage. In addition, the beam diameter has been increased to approximately 1 cm., so that ten electron microscope grids can be exposed to the beam simultaneously.

Tobacco Mosaic Virus-Infected Tissue

1985 ◽  
Vol 43 ◽  
pp. 510-511
Author(s):  
Egbert W. Henry
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Chlorogenic Acid ◽  
Mosaic Virus ◽  
Host Resistance ◽  
Oxidative Metabolism ◽  
Leaf Tissue ◽  
Vein Clearing ◽  
Basal Septum ◽  
Concentration Levels

Tobacco mosaic virus (TMV) infection has been studied in several investigations of Nicotiana tabacum leaf tissue. Earlier studies have suggested that TMV infection does not have precise infective selectivity vs. specific types of tissues. Also, such tissue conditions as vein banding, vein clearing, liquification and suberization may result from causes other than direct TMV infection. At the present time, it is thought that the plasmodesmata, ectodesmata and perhaps the plasmodesmata of the basal septum may represent the actual or more precise sites of TMV infection.TMV infection has been implicated in elevated levels of oxidative metabolism; also, TMV infection may have a major role in host resistance vs. concentration levels of phenolic-type enzymes. Therefore, enzymes such as polyphenol oxidase, peroxidase and phenylalamine ammonia-lyase may show an increase in activity in response to TMV infection. It has been reported that TMV infection may cause a decrease in o-dihydric phenols (chlorogenic acid) in some tissues.

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