scholarly journals Glycosidic Linkage Structures Influence Dietary Fiber Fermentability and Propionate Production by Human Colonic Microbiota In Vitro

2020 ◽  
Vol 15 (10) ◽  
pp. 1900523
Author(s):  
Daisuke Sasaki ◽  
Kengo Sasaki ◽  
Akihiko Kondo
Keyword(s):  
Dietary Fiber ◽  
Glycosidic Linkage ◽  
Colonic Microbiota

scholarly journals Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria

Nutrients ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1495 ◽  
Author(s):  
Aldrine Kilua ◽  
Riri Nomata ◽  
Ryuji Nagata ◽  
Naoki Fukuma ◽  
Kenichiro Shimada ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Chronic Diseases ◽  
Mixed Culture ◽  
Sweet Potato ◽  
Fecal Bacteria ◽  
Microbial Composition ◽  
Colonic Fermentation ◽  
Colonic Microbiota ◽  
Purple Sweet Potato

The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota. In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest. Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP. The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin. Accordingly, PSP might be a material conducive for improving the conditions for the fermentation of partly-fermentable dietary fiber. Besides, PSP was also responsible for the drastic reduction of putrefactive products, especially p-cresol to a significant level. Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.

scholarly journals Subtle Variations in Dietary-Fiber Fine Structure Differentially Influence the Composition and Metabolic Function of Gut Microbiota

mSphere ◽  
2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Yunus E. Tuncil ◽  
Riya D. Thakkar ◽  
Seda Arioglu-Tuncil ◽  
Bruce R. Hamaker ◽  
Stephen R. Lindemann
Keyword(s):  
Community Structure ◽  
Gut Microbiota ◽  
Dietary Fiber ◽  
Fiber Types ◽  
Dietary Fibers ◽  
Soluble Fiber ◽  
Structural Variations ◽  
Colonic Microbiota ◽  
Metabolic Output

ABSTRACT The chemical structures of soluble fiber carbohydrates vary from source to source due to numerous possible linkage configurations among monomers. However, it has not been elucidated whether subtle structural variations might impact soluble fiber fermentation by colonic microbiota. In this study, we tested the hypothesis that subtle structural variations in a soluble polysaccharide govern the community structure and metabolic output of fermenting microbiota. We performed in vitro fecal fermentation studies using arabinoxylans (AXs) from different classes of wheat (hard red spring [AXHRS], hard red winter [AXHRW], and spring red winter [AXSRW]) with identical initial microbiota. Carbohydrate analyses revealed that AXSRW was characterized by a significantly shorter backbone and increased branching compared with those of the hard varieties. Amplicon sequencing demonstrated that fermentation of AXSRW resulted in a distinct community structure of significantly higher richness and evenness than those of hard-AX-fermenting cultures. AXSRW favored OTUs within Bacteroides, whereas AXHRW and AXHRS favored Prevotella. Accordingly, metabolic output varied between hard and soft varieties; higher propionate production was observed with AXSRW and higher butyrate and acetate with AXHRW and AXHRS. This study showed that subtle changes in the structure of a dietary fiber may strongly influence the composition and function of colonic microbiota, further suggesting that physiological functions of dietary fibers are highly structure dependent. Thus, studies focusing on interactions among dietary fiber, gut microbiota, and health outcomes should better characterize the structures of the carbohydrates employed. IMPORTANCE Diet, especially with respect to consumption of dietary fibers, is well recognized as one of the most important factors shaping the colonic microbiota composition. Accordingly, many studies have been conducted to explore dietary fiber types that could predictably manipulate the colonic microbiota for improved health. However, the majority of these studies underappreciate the vastness of fiber structures in terms of their microbial utilization and omit detailed carbohydrate structural analysis. In some cases, this causes conflicting results to arise between studies using (theoretically) the same fibers. In this investigation, by performing in vitro fecal fermentation studies using bran arabinoxylans obtained from different classes of wheat, we showed that even subtle changes in the structure of a dietary fiber result in divergent microbial communities and metabolic outputs. This underscores the need for much higher structural resolution in studies investigating interactions of dietary fibers with gut microbiota, both in vitro and in vivo.

In vitro Inhibition of Pancreatic Enzyme Activities by Dietary Fiber

Digestion ◽  
1982 ◽  
Vol 24 (1) ◽  
pp. 54-59 ◽  
Author(s):  
G. Isaksson ◽  
I. Lundquist ◽  
I. Ihse
Keyword(s):  
Dietary Fiber ◽  
Enzyme Activities ◽  
Pancreatic Enzyme ◽  
In Vitro Inhibition

In vitro starch digestibility, edible quality and microstructure of instant rice noodles enriched with rice bran insoluble dietary fiber

LWT ◽  
2021 ◽  
Vol 142 ◽  
pp. 111008
Author(s):  
Tengnu Liu ◽  
Kang Wang ◽  
Wei Xue ◽  
Li Wang ◽  
Congnan Zhang ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Rice Bran ◽  
Starch Digestibility ◽  
In Vitro Starch Digestibility ◽  
Insoluble Dietary Fiber

scholarly journals Dietary Fiber Modulates the Fermentation Patterns of Cyanidin-3-O-Glucoside in a Fiber-Type Dependent Manner

Foods ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1386
Author(s):  
Zixin Yang ◽  
Ting Huang ◽  
Ping Li ◽  
Jian Ai ◽  
Jiaxin Liu ◽  
...  
Keyword(s):  
Dietary Fiber ◽  
Fiber Type ◽  
Short Chain Fatty Acids ◽  
Dependent Manner ◽  
Dietary Fibers ◽  
Cell Wall Polysaccharides ◽  
Total Carbohydrate ◽  
In Vitro Fermentation ◽  
Gas Volume

The interactions between cell-wall polysaccharides and polyphenols in the gastrointestinal tract have attracted extensive attention. We hypothesized that dietary fiber modulates the fermentation patterns of cyanidin-3-O-glucoside (C3G) in a fiber-type-dependent manner. In the present study, the effects of four dietary fibers (fructose-oligosaccharides, pectin, β-glucan and arabinoxylan) on the modulation of C3G fermentation patterns were investigated through in vitro fermentation inoculated with human feces. The changes in gas volume, pH, total carbohydrate content, metabolites of C3G, antioxidant activity, and microbial community distribution during in vitro fermentation were analyzed. After 24 h of fermentation, the gas volume and total carbohydrate contents of the four dietary-fiber-supplemented groups respectively increased and decreased to varying degrees. The results showed that the C3G metabolites after in vitro fermentation mainly included cyanidin, protocatechuic acid, 2,4,6-trihydroxybenzoic acid, and 2,4,6-trihydroxybenzaldehyde. Supplementation of dietary fibers changed the proportions of C3G metabolites depending on the structures. Dietary fibers increased the production of short-chain fatty acids and the relative abundance of gut microbiota Bifidobacterium and Lactobacillus, thus potentially maintaining colonic health to a certain extent. In conclusion, the used dietary fibers modulate the fermentation patterns of C3G in a fiber-type-dependent manner.

Mo1940 - Establishment of a Stable Human small Intestinal and Colonic Microbiota in an In Vitro Cultivar: Form vs. Function and Response to Oxygen

2018 ◽  
Vol 154 (6) ◽  
pp. S-858-S-859
Author(s):  
Jenni Firrman ◽  
Elliot S. Friedman ◽  
William C. Strange ◽  
Jung-Jin Lee ◽  
Kyle Bittinger ◽  
...  
Keyword(s):  
Colonic Microbiota ◽  
Small Intestinal

scholarly journals DETECTION OF BIOACTIVE FRACTIONS OF JUSTICIA ADHATODA L. LEAVES

2013 ◽  
Vol 3 ◽  
pp. 388
Author(s):  
Faiza Rasheed ◽  
Keyword(s):  
Crude Oil ◽  
Metal Ions ◽  
Dietary Fiber ◽  
Total Alkaloid ◽  
Antioxidant Activities ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Essential Metal ◽  
Justicia Adhatoda

In vitro antibacterial and antioxidant activities of various leaf extracts of Justicia adhatoda L. (locally known as Bhaikar) were assessed. The leaves were also subjected to various phytochemical analysis. Results revealed that leaves of J. adhatoda L. contain significant amount of total alkaloid, phenols flavonoid, saponins, tannins , protein, crude oil, dietary fiber, essential and non essential metal ions. The methanol, ethanol, butanol, chloroform and n-hexane leaf extracts of J

An In Vitro Study on the Effects of Selected Natural Dietary Fiber from Salad Vegetables for Lowering Intestinal Glucose and Lipid Absorption

2021 ◽  
Vol 12 ◽  
Author(s):  
Pannapa Powthong ◽  
Bajaree Jantrapanukorn ◽  
Pattra Suntornthiticharoen ◽  
Chitradee Luprasong
Keyword(s):  
Physical Properties ◽  
Dietary Fiber ◽  
Lipid Absorption ◽  
Postprandial Blood Glucose ◽  
Dependent Manner ◽  
Total Dietary Fiber ◽  
Water Binding ◽  
Breakdown Rates ◽  
Salad Vegetables

Background: Salad vegetables are good sources of dietary fiber and are becoming increasingly popular among consumers. Therefore, these plants have the potential to be developed as functional foods. Objective: Using an in vitro model, this study investigated the physical properties and intestinal glucose and lipid absorption capacities of dry dietary fiber from vegetables typically consumed in salads (types of lettuce, including red oak, red coral, green oak, butterhead, and cos). Method: Fiber was prepared from each type of lettuce using an enzymatic method and then characterized. Physical properties, including solubility and water-binding, swelling, cation-exchange, and oil-binding capacities, and antihyperglycemic and antihypercholesterolemic effects of fiber were investigated. Results: The hydration capacity of total dietary fiber and insoluble fiber from the majority of sources was significantly different from that of cellulose. Adsorption and diffusion of glucose were directly proportional to incubation time, and the diffusion rate was significantly lower in the treatments containing fiber compared to the cellulose control. Fiber from these vegetables also inhibited amylase and alpha-glucosidase activities. Moreover, fiber from all sources exhibited significantly higher sodium cholate and cholesterol-binding capacity compared to cellulose, and also retarded pancreatic cholesterol esterase activity in a concentration-dependent manner. Conclusion: This study demonstrates that natural dietary fiber from salad vegetables can reduce glucose and lipid absorption and breakdown rates, thus preventing increases in postprandial blood glucose and cholesterol levels, which can be beneficial to human health.

scholarly journals Effect of Sweet Corn Residue on Micronutrient Fortification in Baked Cakes

Foods ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 260
Author(s):  
Yu-Xia Lao ◽  
Yu-Ying Yu ◽  
Gao-Ke Li ◽  
Shao-Yun Chen ◽  
Wu Li ◽  
...  
Keyword(s):  
Vitamin E ◽  
Dietary Fiber ◽  
Wheat Flour ◽  
High Performance ◽  
Sweet Corn ◽  
Simulated Digestion ◽  
Corn Residue ◽  
Baked Foods ◽  
Micronutrient Fortification

Owing to the concept of modern life and health, traditional baked foods are seeking transition. In this study, sweet corn residue (SCR) was used to replace wheat flour in cakes. We conducted sensory evaluation and texture analysis to assess sample quality. Also, we simulated digestion in vitro, and determined the content of total sugar and dietary fiber. The content of vitamin E and carotenoids were determined by High Performance Liquid Chromatography (HPLC), and the content of folate was determined by a microbiological method. With the increase of SCR, the content of dietary fiber, folate, vitamin E, and carotenoids significantly increased, and the digestive characteristics improved simultaneously. Based on the above evaluations, SCRC2 (sweet corn residue cake with 60% substitution) had similar sensory quality to the control (pure wheat flour cake) and had the characteristics of slow digestibility and high micronutrients.

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