scholarly journals Ready‐to‐Use Stocks of Agrobacterium tumefaciens Can Simplify Process Development for the Production of Recombinant Proteins by Transient Expression in Plants

2019 ◽  
Vol 14 (10) ◽  
pp. 1900113 ◽  
Author(s):  
Holger Spiegel ◽  
Alexander Boes ◽  
Camil Perales Morales ◽  
Thomas Rademacher ◽  
Johannes F. Buyel
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transient Expression ◽  
Recombinant Proteins ◽  
Process Development

scholarly journals Comparison of Expression Vectors for Transient Expression of Recombinant Proteins in Plants

2013 ◽  
Vol 31 (6) ◽  
pp. 1529-1538 ◽  
Author(s):  
Kausar Hussain Shah ◽  
Bachar Almaghrabi ◽  
Holger Bohlmann
Keyword(s):  
Transient Expression ◽  
Recombinant Proteins ◽  
Expression Vectors

scholarly journals Evaluation of the Influenza A Replicon for Transient Expression of Recombinant Proteins in Mammalian Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (10) ◽  
pp. e13265 ◽  
Author(s):  
Florian Krammer ◽  
Jens Pontiller ◽  
Christopher Tauer ◽  
Dieter Palmberger ◽  
Andreas Maccani ◽  
...  
Keyword(s):  
Transient Expression ◽  
Recombinant Proteins ◽  
Mammalian Cells ◽  
Influenza A

scholarly journals Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

2011 ◽  
Vol 49 (No. 6) ◽  
pp. 255-260 ◽  
Author(s):  
J.I. Flores Solís ◽  
P. Mlejnek ◽  
K. Studená ◽  
S. Procházka
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transient Expression ◽  
Chenopodium Rubrum ◽  
Limiting Factors ◽  
Gus Gene ◽  
Efficient Tool ◽  
Factors Affecting ◽  
Agrobacterium Mediated Transformation ◽  
Glucuronidase Activity ◽  
Gene Coding

Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively enhanced transient expression of GUS gene coding for b-glucuronidase in Chenopodium rubrum. Further the results indicated that the age of seedlings is one of the limiting factors affecting the potency of Agrobacterium tumefaciens infection. Histochemical detection of b-glucuronidase activity revealed that two-days-old seedlings were much more susceptible to infection than ten-days-old ones. According to our results SAAT technology could provide an efficient tool for obtaining stable transformants when applied to two-days-old seedlings of Chenopodium rubrum.

Productivity and Quality of Recombinant Proteins Produced by Stable CHO Cell Clones can be Predicted by Transient Expression in HEK Cells

2012 ◽  
Vol 54 (2) ◽  
pp. 497-503 ◽  
Author(s):  
Carolin Diepenbruck ◽  
Matthias Klinger ◽  
Thomas Urbig ◽  
Patrick Baeuerle ◽  
Rüdiger Neef
Keyword(s):  
Transient Expression ◽  
Recombinant Proteins ◽  
Cho Cell ◽  
Hek Cells ◽  
Cell Clones
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