Construction of Synthetic Promoters by Assembling the Sigma Factor Binding −35 and −10 Boxes

2018 ◽  
pp. 1800298 ◽  
Author(s):  
Yang Wang ◽  
Qingtao Liu ◽  
Huanjiao Weng ◽  
Yanan Shi ◽  
Jian Chen ◽  
...  
Keyword(s):  
Sigma Factor ◽  
Factor Binding ◽  
Synthetic Promoters

scholarly journals Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 inhibit WRKY75 function in abscisic acid-mediated leaf senescence and seed germination

2021 ◽  
Author(s):  
Haiyan Zhang ◽  
Liping Zhang ◽  
Yunrui Ji ◽  
Yifen Jing ◽  
Lanxin Li ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Seed Germination ◽  
Leaf Senescence ◽  
Chromatin Immunoprecipitation ◽  
Sigma Factor ◽  
Dependent Manner ◽  
Negative Regulators ◽  
Factor Binding ◽  
Physiological Processes ◽  
Increased Sensitivity

Abstract The plant-specific VQ gene family participates in diverse physiological processes but little information is available on their role in leaf senescence. Here, we show that the VQ motif-containing proteins, Arabidopsis SIGMA FACTOR BINDING PROTEIN1 (SIB1) and SIB2 are negative regulators of abscisic acid (ABA)-mediated leaf senescence. Loss of SIB1 and SIB2 function resulted in increased sensitivity of ABA-induced leaf senescence. In contrast, overexpression of SIB1 significantly delayed this process. Moreover, biochemical studies revealed that SIBs interact with WRKY75 transcription factor. Loss of WRKY75 function decreased sensitivity to ABA-induced leaf senescence, while overexpression of WRKY75 significantly accelerated this process. Chromatin immunoprecipitation assays revealed that WRKY75 directly binds to the promoters of GOLDEN 2-LIKE1(GLK1) and GLK2, to repress their expression. SIBs repress the transcriptional function of WRKY75 and negatively regulate ABA-induced leaf senescence in a WRKY75-dependent manner. In contrast, WRKY75 positively modulates ABA-mediated leaf senescence in a GLK-dependent manner. In addition, SIBs inhibit WRKY75 function in ABA-mediated seed germination. These results demonstrate that SIBs can form a complex with WRKY75 to regulate ABA-mediated leaf senescence and seed germination.

scholarly journals Further Characterization of ProteolyticClostridium botulinumType A5 Reveals that Neurotoxin Formation Is Unaffected by Loss of thecntR(botR) Promoter Sigma Factor Binding Site

2009 ◽  
Vol 48 (3) ◽  
pp. 1012-1013 ◽  
Author(s):  
Andrew T. Carter ◽  
David R. Mason ◽  
Kathie A. Grant ◽  
Giovanna Franciosa ◽  
Paulo Aureli ◽  
...  
Keyword(s):  
Binding Site ◽  
Sigma Factor ◽  
Factor Binding Site ◽  
Factor Binding

scholarly journals SIGffRid: A tool to search for sigma factor binding sites in bacterial genomes using comparative approach and biologically driven statistics

2008 ◽  
Vol 9 (1) ◽  
Author(s):  
Fabrice Touzain ◽  
Sophie Schbath ◽  
Isabelle Debled-Rennesson ◽  
Bertrand Aigle ◽  
Gregory Kucherov ◽  
...  
Keyword(s):  
Binding Sites ◽  
Sigma Factor ◽  
Comparative Approach ◽  
Bacterial Genomes ◽  
Factor Binding

scholarly journals Engineering strong and stress-responsive promoters in Bacillus subtilis by interlocking sigma factor binding motifs

2019 ◽  
Vol 4 (4) ◽  
pp. 197-203 ◽  
Author(s):  
Yang Wang ◽  
Yanan Shi ◽  
Litao Hu ◽  
Guocheng Du ◽  
Jian Chen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Sigma Factor ◽  
Binding Motifs ◽  
Factor Binding

Identification and Structure of the Anti-sigma Factor-binding Domain of the Disulphide-stress Regulated Sigma Factor σR from Streptomyces coelicolor

2002 ◽  
Vol 323 (2) ◽  
pp. 225-236 ◽  
Author(s):  
Wei Li ◽  
Clare E.M. Stevenson ◽  
Nicolas Burton ◽  
Piotr Jakimowicz ◽  
Mark S.B. Paget ◽  
...  
Keyword(s):  
Sigma Factor ◽  
Streptomyces Coelicolor ◽  
Binding Domain ◽  
Factor Binding

scholarly journals N-Terminal Acetylation Stabilizes SIGMA FACTOR BINDING PROTEIN1 Involved in Salicylic Acid-Primed Cell Death

2020 ◽  
Vol 183 (1) ◽  
pp. 358-370 ◽  
Author(s):  
Zihao Li ◽  
Vivek Dogra ◽  
Keun Pyo Lee ◽  
Rongxia Li ◽  
Mingyue Li ◽  
...  
Keyword(s):  
Cell Death ◽  
Salicylic Acid ◽  
Sigma Factor ◽  
Factor Binding ◽  
Primed Cell

scholarly journals Arabidopsis Sigma Factor Binding Proteins Are Activators of the WRKY33 Transcription Factor in Plant Defense

2011 ◽  
Vol 23 (10) ◽  
pp. 3824-3841 ◽  
Author(s):  
Zhibing Lai ◽  
Ying Li ◽  
Fei Wang ◽  
Yuan Cheng ◽  
Baofang Fan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Plant Defense ◽  
Sigma Factor ◽  
Binding Proteins ◽  
Factor Binding
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