scholarly journals Enzymatic Adaptation of Podospora anserina to Different Plant Biomass Provides Leads to Optimized Commercial Enzyme Cocktails

2018 ◽  
Vol 14 (4) ◽  
pp. 1800185 ◽  
Author(s):  
Tiziano Benocci ◽  
Paul Daly ◽  
Maria Victoria Aguilar‐Pontes ◽  
Kathleen Lail ◽  
Mei Wang ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Podospora Anserina ◽  
Commercial Enzyme ◽  
Enzymatic Adaptation

Plant biomass degrading ability of the coprophilic ascomycete fungus Podospora anserina

2016 ◽  
Vol 34 (5) ◽  
pp. 976-983 ◽  
Author(s):  
Marie Couturier ◽  
Narumon Tangthirasunun ◽  
Xie Ning ◽  
Sylvain Brun ◽  
Valérie Gautier ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Podospora Anserina

scholarly journals Inactivation of Cellobiose Dehydrogenases Modifies the Cellulose Degradation Mechanism of Podospora anserina

2016 ◽  
Vol 83 (2) ◽  
Author(s):  
Narumon Tangthirasunun ◽  
David Navarro ◽  
Sona Garajova ◽  
Didier Chevret ◽  
Laetitia Chan Ho Tong ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Cellulose Degradation ◽  
Degradation Mechanism ◽  
Podospora Anserina ◽  
Cellulosic Biomass ◽  
Striking Difference ◽  
Crystalline Cellulose ◽  
Minor Role ◽  
Wild Type ◽  
Content Type

ABSTRACT Conversion of biomass into high-value products, including biofuels, is of great interest to developing sustainable biorefineries. Fungi are an inexhaustible source of enzymes to degrade plant biomass. Cellobiose dehydrogenases (CDHs) play an important role in the breakdown through synergistic action with fungal lytic polysaccharide monooxygenases (LPMOs). The three CDH genes of the model fungus Podospora anserina were inactivated, resulting in single and multiple CDH mutants. We detected almost no difference in growth and fertility of the mutants on various lignocellulose sources, except on crystalline cellulose, on which a 2-fold decrease in fertility of the mutants lacking P. anserina CDH1 (PaCDH1) and PaCDH2 was observed. A striking difference between wild-type and mutant secretomes was observed. The secretome of the mutant lacking all CDHs contained five beta-glucosidases, whereas the wild type had only one. P. anserina seems to compensate for the lack of CDH with secretion of beta-glucosidases. The addition of P. anserina LPMO to either the wild-type or mutant secretome resulted in improvement of cellulose degradation in both cases, suggesting that other redox partners present in the mutant secretome provided electrons to LPMOs. Overall, the data showed that oxidative degradation of cellulosic biomass relies on different types of mechanisms in fungi. IMPORTANCE Plant biomass degradation by fungi is a complex process involving dozens of enzymes. The roles of each enzyme or enzyme class are not fully understood, and utilization of a model amenable to genetic analysis should increase the comprehension of how fungi cope with highly recalcitrant biomass. Here, we report that the cellobiose dehydrogenases of the model fungus Podospora anserina enable it to consume crystalline cellulose yet seem to play a minor role on actual substrates, such as wood shavings or miscanthus. Analysis of secreted proteins suggests that Podospora anserina compensates for the lack of cellobiose dehydrogenase by increasing beta-glucosidase expression and using an alternate electron donor for LPMO.

scholarly journals Appressorium: The Breakthrough in Dikarya

2019 ◽  
Vol 5 (3) ◽  
pp. 72 ◽  
Author(s):  
Alexander Demoor ◽  
Philippe Silar ◽  
Sylvain Brun
Keyword(s):  
Filamentous Fungi ◽  
Mycorrhizal Fungi ◽  
Large Range ◽  
Plant Biomass ◽  
Podospora Anserina ◽  
Evolutionary Origin ◽  
Saprotrophic Fungi ◽  
Mutualistic Fungi ◽  
Dead Plant

Phytopathogenic and mycorrhizal fungi often penetrate living hosts by using appressoria and related structures. The differentiation of similar structures in saprotrophic fungi to penetrate dead plant biomass has seldom been investigated and has been reported only in the model fungus Podospora anserina. Here, we report on the ability of many saprotrophs from a large range of taxa to produce appressoria on cellophane. Most Ascomycota and Basidiomycota were able to form appressoria. In contrast, none of the three investigated Mucoromycotina was able to differentiate such structures. The ability of filamentous fungi to differentiate appressoria no longer belongs solely to pathogenic or mutualistic fungi, and this raises the question of the evolutionary origin of the appressorium in Eumycetes.

scholarly journals Cultivation of Podospora anserina on soybean hulls results in an efficient enzyme cocktail for plant biomass hydrolysis

2017 ◽  
Vol 37 ◽  
pp. 162-171 ◽  
Author(s):  
Miia R. Mäkelä ◽  
Ourdia Bouzid ◽  
Diogo Robl ◽  
Harm Post ◽  
Mao Peng ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Podospora Anserina ◽  
Soybean Hulls ◽  
Biomass Hydrolysis ◽  
Enzyme Cocktail

ESTIMATION OF ABOVE GROUND BIOMASS OF THE THREE SITES (GRAZED SITE, PROTECTED SITE AND SEED SOWN SITE) FOR COMPARING THEIR PRODUCTIVITY IN KASHMIR VALLEY

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
AFSHAN ANJUM BABA ◽  
SYED NASEEM UL-ZAFAR GEELANI ◽  
ISHRAT SALEEM ◽  
MOHIT HUSAIN ◽  
PERVEZ AHMAD KHAN ◽  
...  
Keyword(s):  
Protected Areas ◽  
Aboveground Biomass ◽  
Plant Biomass ◽  
Summer Season ◽  
Above Ground Biomass ◽  
Spring Season ◽  
Kashmir Valley ◽  
Ground Biomass ◽  
Maximum Value ◽  
Protected Site

The plant biomass for protected areas was maximum in summer (1221.56 g/m2) and minimum in winter (290.62 g/m2) as against grazed areas having maximum value 590.81 g/m2 in autumn and minimum 183.75 g/m2 in winter. Study revealed that at Protected site (Kanidajan) the above ground biomass ranged was from a minimum (1.11 t ha-1) in the spring season to a maximum (4.58 t ha-1) in the summer season while at Grazed site (Yousmarag), the aboveground biomass varied from a minimum (0.54 t ha-1) in the spring season to a maximum of 1.48 t ha-1 in summer seasonandat Seed sown site (Badipora), the lowest value of aboveground biomass obtained was 4.46 t ha-1 in spring while as the highest (7.98 t ha-1) was obtained in summer.

REED - PLANT BIOMASS - AS RENEWABLE AND LOW-POLLUTING ENERGY RESOURCE

2011 ◽  
Vol 10 (8) ◽  
pp. 1053-1057
Author(s):  
Gavril Budau ◽  
Mihaela Campean ◽  
Camelia Cosereanu ◽  
Dumitru Lica
Keyword(s):  
Plant Biomass ◽  
Energy Resource

МАТЕМАТИЧЕСКОЕ МОДЕЛИРОВАНИЕ ДИНАМИКИ МИНЕРАЛЬНОГО ПИТАНИЯ РАСТЕНИЙ В СИСТЕМЕ «УДОБРЕНИЕ-ПОЧВА-РАСТЕНИЕ»

2020 ◽  
Vol 65 (6) ◽  
pp. 1219-1229
Author(s):  
В.А. Четырбоцкий ◽  
◽  
А.Н. Четырбоцкий ◽  
Б.В. Левин ◽  
◽  
...  
Keyword(s):  
Experimental Data ◽  
Temporal Dynamics ◽  
Plant Biomass ◽  
Environmental Parameters ◽  
Parametric Identification ◽  
Agricultural Crop ◽  
Parameter System ◽  
Plant Nitrogen ◽  
Rhizosphere Microorganisms ◽  
Nitrogen Phosphorus

A numerical simulation of the spatial-temporal dynamics of a multi-parameter system is developed. The components of this system are plant biomass, mobile and stationary forms of mineral nutrition elements, rhizosphere microorganisms and environmental parameters (temperature, humidity, acidity). Parametric identification and verification of the adequacy of the model were carried out based on the experimental data on the growth of spring wheat «Krasnoufimskaya-100» on peat lowland soil. The results are represented by temporal distributions of biomass from agricultural crop under study and the findings on the content of main nutrition elements within the plant (nitrogen, phosphorus, potassium). An agronomic assessment and interpretation of the obtained results are given.

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