Perfusion Cell Culture Decreases Process and Product Heterogeneity in a Head‐to‐Head Comparison With Fed‐Batch

2018 ◽  
Vol 14 (2) ◽  
pp. 1700733 ◽  
Author(s):  
Jason Walther ◽  
Jiuyi Lu ◽  
Myles Hollenbach ◽  
Marcella Yu ◽  
Chris Hwang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fed Batch ◽  
Perfusion Cell Culture ◽  
Product Heterogeneity

Anti-apoptotic genes Aven and E1B-19K enhance performance of BHK cells engineered to express recombinant factor VIII in batch and low perfusion cell culture

2007 ◽  
Vol 98 (4) ◽  
pp. 825-841 ◽  
Author(s):  
Toey Nivitchanyong ◽  
Amanda Martinez ◽  
Adiba Ishaque ◽  
John E. Murphy ◽  
Konstantin Konstantinov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Factor Viii ◽  
Recombinant Factor Viii ◽  
Perfusion Cell Culture ◽  
Bhk Cells ◽  
Apoptotic Genes

Investigation of metabolic variability observed in extended fed batch cell culture

2013 ◽  
Vol 29 (6) ◽  
pp. 1519-1527 ◽  
Author(s):  
Alan Gilbert ◽  
Kyle McElearney ◽  
Rashmi Kshirsagar ◽  
Martin S. Sinacore ◽  
Thomas Ryll
Keyword(s):  
Cell Culture ◽  
Fed Batch ◽  
Batch Cell

Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture

2016 ◽  
pp. 161-176 ◽  
Author(s):  
Gabor Nagy ◽  
Bence Tanczos ◽  
Eszter Fidrus ◽  
Laszlo Talas ◽  
Gaspar Banfalvi
Keyword(s):  
Cell Cycle ◽  
Cell Culture ◽  
Cell Cycle Arrest ◽  
Perfusion Cell Culture ◽  
Cycle Arrest ◽  
Chemically Induced

scholarly journals Mathematical modelling of fluid flow and solute transport to define operating parameters for in vitro perfusion cell culture systems

2020 ◽  
Vol 10 (2) ◽  
pp. 20190045 ◽  
Author(s):  
Lauren Hyndman ◽  
Sean McKee ◽  
Nigel J. Mottram ◽  
Bhumika Singh ◽  
Steven D. Webb ◽  
...  
Keyword(s):  
Cell Culture ◽  
Fluid Flow ◽  
Mathematical Modelling ◽  
Solute Transport ◽  
Computational Modelling ◽  
Operating Parameters ◽  
Perfusion Cell Culture ◽  
Culture Systems ◽  
Cell Culture Systems

In recent years, there has been a move away from the use of static in vitro two-dimensional cell culture models for testing the chemical safety and efficacy of drugs. Such models are increasingly being replaced by more physiologically relevant cell culture systems featuring dynamic flow and/or three-dimensional structures of cells. While it is acknowledged that such systems provide a more realistic environment within which to test drugs, progress is being hindered by a lack of understanding of the physical and chemical environment that the cells are exposed to. Mathematical and computational modelling may be exploited in this regard to unravel the dependency of the cell response on spatio-temporal differences in chemical and mechanical cues, thereby assisting with the understanding and design of these systems. In this paper, we present a mathematical modelling framework that characterizes the fluid flow and solute transport in perfusion bioreactors featuring an inlet and an outlet. To demonstrate the utility of our model, we simulated the fluid dynamics and solute concentration profiles for a variety of different flow rates, inlet solute concentrations and cell types within a specific commercial bioreactor chamber. Our subsequent analysis has elucidated the basic relationship between inlet flow rate and cell surface flow speed, shear stress and solute concentrations, allowing us to derive simple but useful relationships that enable prediction of the behaviour of the system under a variety of experimental conditions, prior to experimentation. We describe how the model may used by experimentalists to define operating parameters for their particular perfusion cell culture systems and highlight some operating conditions that should be avoided. Finally, we critically comment on the limitations of mathematical and computational modelling in this field, and the challenges associated with the adoption of such methods.

LLC-PK1 Cells Maintained in a New Perfusion Cell Culture System Exhibit an Improved Oxidative Metabolism

2002 ◽  
Vol 12 (2-3) ◽  
pp. 153-162 ◽  
Author(s):  
Edward Felder ◽  
Paul Jennings ◽  
Thomas Seppi ◽  
Walter Pfaller
Keyword(s):  
Cell Culture ◽  
Oxidative Metabolism ◽  
Culture System ◽  
Cell Culture System ◽  
Perfusion Cell Culture
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