Improved Performance in Mammalian Cell Perfusion Cultures by Growth Inhibition

2018 ◽  
Vol 14 (2) ◽  
pp. 1700722 ◽  
Author(s):  
Moritz K. F Wolf ◽  
Aurélie Closet ◽  
Monika Bzowska ◽  
Jean‐Marc Bielser ◽  
Jonathan Souquet ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mammalian Cell ◽  
Perfusion Cultures ◽  
Improved Performance

OUR AND CER ESTIMATION IN HIGH DENSITY MAMMALIAN CELL PERFUSION CULTURES

2007 ◽  
Vol 40 (4) ◽  
pp. 91-96 ◽  
Author(s):  
C. Goudar ◽  
K. Joeris ◽  
C. Cruz ◽  
C. Zhang ◽  
K. Konstantinov
Keyword(s):  
Mammalian Cell ◽  
High Density ◽  
Perfusion Cultures

Oxygen Uptake Rate (OUR) Estimation in High Density Mammalian Cell Perfusion Cultures

2010 ◽  
pp. 837-841 ◽  
Author(s):  
C. Cruz ◽  
K. Joeris ◽  
C. Goudar ◽  
C. Zhang ◽  
K. Konstantinov
Keyword(s):  
Oxygen Uptake ◽  
Mammalian Cell ◽  
Uptake Rate ◽  
Oxygen Uptake Rate ◽  
High Density ◽  
Perfusion Cultures

scholarly journals RACK1 Interacts with E1A and Rescues E1A-induced Yeast Growth Inhibition and Mammalian Cell Apoptosis

2001 ◽  
Vol 276 (29) ◽  
pp. 27026-27033 ◽  
Author(s):  
Nianli Sang ◽  
Anna Severino ◽  
Patrizia Russo ◽  
Alfonso Baldi ◽  
Antonio Giordano ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mammalian Cell ◽  
Cell Apoptosis ◽  
Yeast Growth ◽  
Yeast Growth Inhibition

Toward The Simultaneous Correction of Spherical and Chromatic Aberration in Electron Optics by Means of an Electrostatic Electron Mirror

1990 ◽  
Vol 48 (1) ◽  
pp. 206-207
Author(s):  
Gertrude. F. Rempfer
Keyword(s):  
Chromatic Aberration ◽  
Transverse Magnetic ◽  
Objective Lens ◽  
Ion Imaging ◽  
Corrected Image ◽  
Electric And Magnetic Fields ◽  
Chromatic Aberrations ◽  
Improved Performance ◽  
Electron Microscopes ◽  
Image Planes

Optimum performance in electron and ion imaging instruments, such as electron microscopes and probe-forming instruments, in most cases depends on a compromise either between imaging errors due to spherical and chromatic aberrations and the diffraction error or between the imaging errors and the current in the image. These compromises result in the use of very small angular apertures. Reducing the spherical and chromatic aberration coefficients would permit the use of larger apertures with resulting improved performance, granted that other problems such as incorrect operation of the instrument or spurious disturbances do not interfere. One approach to correcting aberrations which has been investigated extensively is through the use of multipole electric and magnetic fields. Another approach involves the use of foil windows. However, a practical system for correcting spherical and chromatic aberration is not yet available.Our approach to correction of spherical and chromatic aberration makes use of an electrostatic electron mirror. Early studies of the properties of electron mirrors were done by Recknagel. More recently my colleagues and I have studied the properties of the hyperbolic electron mirror as a function of the ratio of accelerating voltage to mirror voltage. The spherical and chromatic aberration coefficients of the mirror are of opposite sign (overcorrected) from those of electron lenses (undercorrected). This important property invites one to find a way to incorporate a correcting mirror in an electron microscope. Unfortunately, the parts of the beam heading toward and away from the mirror must be separated. A transverse magnetic field can separate the beams, but in general the deflection aberrations degrade the image. The key to avoiding the detrimental effects of deflection aberrations is to have deflections take place at image planes. Our separating system is shown in Fig. 1. Deflections take place at the separating magnet and also at two additional magnetic deflectors. The uncorrected magnified image formed by the objective lens is focused in the first deflector, and relay lenses transfer the image to the separating magnet. The interface lens and the hyperbolic mirror acting in zoom fashion return the corrected image to the separating magnet, and the second set of relay lenses transfers the image to the final deflector, where the beam is deflected onto the projection axis.

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