scholarly journals An Efficient Transformation Method for the Bioplastic-Producing “Knallgas” Bacterium Ralstonia eutropha H16

2017 ◽  
Vol 12 (11) ◽  
pp. 1700081 ◽  
Author(s):  
Kang Lan Tee ◽  
James Grinham ◽  
Arona M. Othusitse ◽  
Miriam González-Villanueva ◽  
Abayomi O. Johnson ◽  
...  
Keyword(s):  
Ralstonia Eutropha ◽  
Transformation Method ◽  
Efficient Transformation ◽  
Ralstonia Eutropha H16

CbbR and RegA regulate cbb operon transcription in Ralstonia eutropha H16

2017 ◽  
Vol 257 ◽  
pp. 78-86 ◽  
Author(s):  
Steffen Gruber ◽  
Helmut Schwab ◽  
Petra Heidinger
Keyword(s):  
Ralstonia Eutropha ◽  
Ralstonia Eutropha H16

Characterization of propionate CoA-transferase from Ralstonia eutropha H16

2013 ◽  
Vol 98 (8) ◽  
pp. 3579-3589 ◽  
Author(s):  
Elena Volodina ◽  
Marc Schürmann ◽  
Nicole Lindenkamp ◽  
Alexander Steinbüchel
Keyword(s):  
Ralstonia Eutropha ◽  
Coa Transferase ◽  
Ralstonia Eutropha H16

scholarly journals Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite

AMB Express ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 59 ◽  
Author(s):  
Daniel Heinrich ◽  
Mohamed H Madkour ◽  
Mansour A Al-Ghamdi ◽  
Ibraheem I Shabbaj ◽  
Alexander Steinbüchel
Keyword(s):  
Sodium Hypochlorite ◽  
Large Scale ◽  
Ralstonia Eutropha ◽  
Ralstonia Eutropha H16

Metabolite profiles of polyhydroxyalkanoate-producing Ralstonia eutropha H16

Metabolomics ◽  
2013 ◽  
Vol 10 (2) ◽  
pp. 190-202 ◽  
Author(s):  
Toshiaki Fukui ◽  
Kenta Chou ◽  
Kazuo Harada ◽  
Izumi Orita ◽  
Yasumune Nakayama ◽  
...  
Keyword(s):  
Ralstonia Eutropha ◽  
Metabolite Profiles ◽  
Ralstonia Eutropha H16

Engineering the CBB cycle and hydrogen utilization pathway of Ralstonia eutropha H16 for improved autotrophic growth and PHB production

2020 ◽  
Author(s):  
Zhongkang Li ◽  
Muzi Hu ◽  
Bin Xiong ◽  
Dongdong Zhao ◽  
Chunzhi Zhang ◽  
...  
Keyword(s):  
Carbon Fixation ◽  
Ralstonia Eutropha ◽  
Biological Research ◽  
Industrial Biotechnology ◽  
Cell Factory ◽  
Autotrophic Growth ◽  
Microbial Cell Factory ◽  
Knock Out ◽  
Ralstonia Eutropha H16 ◽  
Living Organisms

Abstract CO 2 is fixed by all living organisms with an autotrophic metabolism, among which the Calvin-Benson-Bassham ( CBB) cycle is the most important and widespread carbon fixation pathway. Thus, studying and engineering the CBB cycle with the associated energy providing pathways to increase the CO 2 fixation efficiency of cells is an important subject of biological research with significant application potential. In this work, the autotrophic microbe Ralstonia eutropha H16 was selected as a research platform for CBB cycle optimization engineering. By knocking out either CBB operon genes on the operon or mega-plasmid of R. eutropha , we found that both CBB operons were active and contributed almost equally to the carbon fixation process. With similar knock-out experiments, we found while both soluble and membrane-bound hydrogenases (SH and MBH), belonging to the energy providing hydrogenase module, were f unctional d uring autotrophic growth of R. eutropha. And SH played a more significant role. By introducing a heterologous cyanobacterial RuBisCO with the endogenous GroES/EL chaperone system and RbcX, the culture OD 600 of engineered strain increased 89.15% after 72 hours of autotrophic growth, indicating cyanobacterial RuBisCO with a higher activity was functional in R. eutropha and improved upon original CBB pathway. Meanwhile, expression of hydrogenases were optimized by modulating the expression of MBH and SH, which could further increase the R. eutropha H16 culture OD 600 to 93.4% at 72 hours. Moreover, the autotrophic yield of its major industrially relevant product, polyhydroxybutyrate (PHB), was increased by 99.71%. To our best knowledge, this is the first report of successfully engineering the CBB pathway of R. eutropha for improved activity , and is one of only a few cases where the efficiency of CO 2 assimilation pathway was improved. Our work demonstrates that R. eutropha is an extremely useful platform for studying and engineering the CBB for applications in more important organisms, such as agricultural crops, and a potential microbial cell factory to develop industrial biotechnology for sequestrating CO 2 .

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