Scaling up a chemically-defined aggregate-based suspension culture system for neural commitment of human pluripotent stem cells

2016 ◽  
Vol 11 (12) ◽  
pp. 1628-1638 ◽  
Author(s):  
Cláudia C. Miranda ◽  
Tiago G. Fernandes ◽  
M. Margarida Diogo ◽  
Joaquim M.S. Cabral
Keyword(s):  
Stem Cells ◽  
Suspension Culture ◽  
Pluripotent Stem Cells ◽  
Culture System ◽  
Scaling Up ◽  
Human Pluripotent Stem Cells

scholarly journals Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System

2016 ◽  
Vol 6 (6) ◽  
pp. 993-1008 ◽  
Author(s):  
Alessandra Rigamonti ◽  
Giuliana G. Repetti ◽  
Chicheng Sun ◽  
Feodor D. Price ◽  
Danielle C. Reny ◽  
...  
Keyword(s):  
Stem Cells ◽  
Suspension Culture ◽  
Pluripotent Stem Cells ◽  
Culture System ◽  
Large Scale ◽  
Three Dimensional ◽  
Human Pluripotent Stem Cells ◽  
Scale Production ◽  
Large Scale Production ◽  
Mature Neurons

Scalable expansion of human pluripotent stem cells in suspension culture

2011 ◽  
Vol 6 (5) ◽  
pp. 689-700 ◽  
Author(s):  
Robert Zweigerdt ◽  
Ruth Olmer ◽  
Harmeet Singh ◽  
Axel Haverich ◽  
Ulrich Martin
Keyword(s):  
Stem Cells ◽  
Suspension Culture ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

Chemically defined and growth-factor-free culture system for the expansion and derivation of human pluripotent stem cells

2018 ◽  
Vol 2 (3) ◽  
pp. 173-182 ◽  
Author(s):  
Shin-ya Yasuda ◽  
Tatsuhiko Ikeda ◽  
Hosein Shahsavarani ◽  
Noriko Yoshida ◽  
Bhavana Nayer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Pluripotent Stem Cells ◽  
Culture System ◽  
Human Pluripotent Stem Cells

Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells

2011 ◽  
Vol 6 (5) ◽  
pp. 572-579 ◽  
Author(s):  
Michal Amit ◽  
Ilana Laevsky ◽  
Yael Miropolsky ◽  
Kohava Shariki ◽  
Meital Peri ◽  
...  
Keyword(s):  
Stem Cells ◽  
Suspension Culture ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

scholarly journals Aggregate formation and suspension culture of human pluripotent stem cells and differentiated progeny

Methods ◽  
2016 ◽  
Vol 101 ◽  
pp. 11-20 ◽  
Author(s):  
Tracy A. Hookway ◽  
Jessica C. Butts ◽  
Emily Lee ◽  
Hengli Tang ◽  
Todd C. McDevitt
Keyword(s):  
Stem Cells ◽  
Suspension Culture ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Aggregate Formation

scholarly journals Generation of cortical neurons through large-scale expanding neuroepithelial stem cell from human pluripotent stem cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Shumei Zhao ◽  
Kui Duan ◽  
Zongyong Ai ◽  
Baohua Niu ◽  
Yanying Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Suspension Culture ◽  
Pluripotent Stem Cells ◽  
Cortical Neurons ◽  
Culture System ◽  
Large Scale ◽  
Disease Modeling ◽  
High Quality ◽  
Differentiation Potential

Abstract Background Considerable progress has been made in converting human pluripotent stem cells (hPSCs) into cortical neurons for disease modeling and regenerative medicine. However, these procedures are hard to provide sufficient cells for their applications. Using a combination of small-molecules and growth factors, we previously identified one condition which can rapidly induce hPSCs into neuroepithelial stem cells (NESCs). Here, we developed a scalable suspension culture system, which largely yields high-quality NESC-spheres and subsequent cortical neurons. Methods The NESC medium was first optimized, and the suspension culture system was then enlarged from plates to stirred bioreactors for large-scale production of NESC-spheres by a stirring speed of 60 rpm. During the expansion, the quality of NESC-spheres was evaluated. The differentiation potential of NESC-spheres into cortical neurons was demonstrated by removing bFGF and two pathway inhibitors from the NESC medium. Cellular immunofluorescence staining, global transcriptome, and single-cell RNA sequencing analysis were used to identify the characteristics, identities, purities, or homogeneities of NESC-spheres or their differentiated cells, respectively. Results The optimized culture system is more conducive to large-scale suspension production of NESCs. These largely expanded NESC-spheres maintain unlimited self-renewal ability and NESC state by retaining their uniform sizes, high cell vitalities, and robust expansion abilities. After long-term expansion, NESC-spheres preserve high purity, homogeneity, and normal diploid karyotype. These expanded NESC-spheres on a large scale have strong differentiation potential and effectively produce mature cortical neurons. Conclusions We developed a serum-free, defined, and low-cost culture system for large-scale expansion of NESCs in stirred suspension bioreactors. The stable and controllable 3D system supports long-term expansion of high-quality and homogeneous NESC-spheres. These NESC-spheres can be used to efficiently give rise to cortical neurons for cell therapy, disease modeling, and drug screening in future.

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