scholarly journals Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation inE. coli

2017 ◽  
Vol 12 (3) ◽  
pp. 1600415 ◽  
Author(s):  
Mingzhi Liang ◽  
Stefanie Frank ◽  
Heinrich Lünsdorf ◽  
Martin J. Warren ◽  
Michael B. Prentice
Keyword(s):  
Polyphosphate Kinase ◽  
Bacterial Microcompartment ◽  
Polyphosphate Accumulation

Polyphosphate Kinase Activity during Phosphate Uptake by Bacteria

1985 ◽  
Vol 17 (11-12) ◽  
pp. 43-56 ◽  
Author(s):  
J. T'Seyen ◽  
D. Malnou ◽  
J. C. Block ◽  
G. Faup
Keyword(s):  
Enzyme Activity ◽  
Activated Sludge ◽  
Pure Culture ◽  
Kinase Activity ◽  
Phosphate Uptake ◽  
Bacterial Species ◽  
Research Field ◽  
Polyphosphate Kinase ◽  
Polyphosphate Accumulation ◽  
Inhibitory Molecules

Polyphosphate kinase, one of the key enzymes for polyphosphate accumulation in bacteria, has been investigated in pure culture and activated sludge samples. Three bacterial species (Acinetobacter lwoffi, A. phosphadevorus and Pseudomonas fluorescens) appeared to have a polyphosphate kinase activity (between 0.3 and 4.7 nM of P04 transferred per minute and per mg of proteins). However tests carried out on Aeromonas hydrophila, Bacillus subtilis, E. coli and Serratia sp. showed these species did not have any measurable polyphosphate kinase activity. Enzyme activity in bacteria is dependent on environmental conditions and can be induced, in particular, by anaerobic stress, even without modification of extracellular phosphate concentrations. Most of the activated sludge samples taken from laboratory pilot plants achieving good phosphorus removal did not present any measurable polyphosphate kinase activity. Such poor results can be related to low numbers of phosphorus removing bacteria and inhibitory molecules in sludge extracts. The only observed activity was in pretreated sludges (washed sludges) and in sludges submitted in a batch reator to phosphate starvation. However, in these, enzyme activity was at least 20 times lower than those measured in pure culture of A. lwoffi. Polyphosphate kinase induction is a complicated and time consuming method which has to be used only in the research field. At the present time our results show that sludge phosphate uptake potential cannot be predicted by the measure of induction of polyphosphate kinase activity.

Bacterial Polyphosphate Kinases Revisited: Role in Pathogenesis and Therapeutic Potential

2019 ◽  
Vol 20 (3) ◽  
pp. 292-301 ◽  
Author(s):  
Lalit Kumar Gautam ◽  
Prince Sharma ◽  
Neena Capalash
Keyword(s):  
Drug Target ◽  
Bacterial Infections ◽  
Therapeutic Potential ◽  
Wide Distribution ◽  
Medical Community ◽  
Polyphosphate Kinase ◽  
Potential Drug Target ◽  
Potential Drug ◽  
Resistant Strains ◽  
Structural Aspects

Bacterial infections have always been an unrestrained challenge to the medical community due to the rise of multi-drug tolerant and resistant strains. Pioneering work on Escherichia coli polyphosphate kinase (PPK) by Arthur Kornberg has generated great interest in this polyphosphate (PolyP) synthesizing enzyme. PPK has wide distribution among pathogens and is involved in promoting pathogenesis, stress management and susceptibility to antibiotics. Further, the absence of a PPK orthologue in humans makes it a potential drug target. This review covers the functional and structural aspects of polyphosphate kinases in bacterial pathogens. A description of molecules being designed against PPKs has been provided, challenges associated with PPK inhibitor design are highlighted and the strategies to enable development of efficient drug against this enzyme have also been discussed.

Substrate channels revealed in the trimericLactobacillus reuteribacterial microcompartment shell protein PduB

2012 ◽  
Vol 68 (12) ◽  
pp. 1642-1652 ◽  
Author(s):  
Allan Pang ◽  
Mingzhi Liang ◽  
Michael B. Prentice ◽  
Richard W. Pickersgill
Keyword(s):  
Tandem Repeats ◽  
Lactobacillus Reuteri ◽  
Protein Complexes ◽  
Protein Subunits ◽  
Shell Protein ◽  
Domain Structures ◽  
Bacterial Microcompartment ◽  
Major Shell ◽  
Carbon Molecules ◽  
Trimeric Structure

Lactobacillus reuterimetabolizes two similar three-carbon molecules, 1,2-propanediol and glycerol, within closed polyhedral subcellular bacterial organelles called bacterial microcompartments (metabolosomes). The outer shell of the propanediol-utilization (Pdu) metabolosome is composed of hundreds of mainly hexagonal protein complexes made from six types of protein subunits that share similar domain structures. The structure of the bacterial microcompartment protein PduB has a tandem structural repeat within the subunit and assembles into a trimer with pseudo-hexagonal symmetry. This trimeric structure forms sheets in the crystal lattice and is able to fit within a polymeric sheet of the major shell component PduA to assemble a facet of the polyhedron. There are three pores within the trimer and these are formed between the tandem repeats within the subunits. The structure shows that each of these pores contains three glycerol molecules that interact with conserved residues, strongly suggesting that these subunit pores channel glycerol substrate into the metabolosome. In addition to the observation of glycerol occupying the subunit channels, the presence of glycerol on the molecular threefold symmetry axis suggests a role in locking closed the central region.

scholarly journals Characteristic Microbiomes Correlate with Polyphosphate Accumulation of Marine Sponges in South China Sea Areas

2019 ◽  
Vol 8 (1) ◽  
pp. 63
Author(s):  
Huilong Ou ◽  
Mingyu Li ◽  
Shufei Wu ◽  
Linli Jia ◽  
Russell T. Hill ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Inorganic Phosphorus ◽  
Marine Sponges ◽  
Strongly Correlated ◽  
Mantel Tests ◽  
Low Concentrations ◽  
Enrichment Rate ◽  
Total Dna ◽  
Polyphosphate Accumulation ◽  
Sequence Similarities

Some sponges have been shown to accumulate abundant phosphorus in the form of polyphosphate (polyP) granules even in waters where phosphorus is present at low concentrations. But the polyP accumulation occurring in sponges and their symbiotic bacteria have been little studied. The amounts of polyP exhibited significant differences in twelve sponges from marine environments with high or low dissolved inorganic phosphorus (DIP) concentrations which were quantified by spectral analysis, even though in the same sponge genus, e.g., Mycale sp. or Callyspongia sp. PolyP enrichment rates of sponges in oligotrophic environments were far higher than those in eutrophic environments. Massive polyP granules were observed under confocal microscopy in samples from very low DIP environments. The composition of sponge symbiotic microbes was analyzed by high-throughput sequencing and the corresponding polyphosphate kinase (ppk) genes were detected. Sequence analysis revealed that in the low DIP environment, those sponges with higher polyP content and enrichment rates had relatively higher abundances of cyanobacteria. Mantel tests and canonical correspondence analysis (CCA) examined that the polyP enrichment rate was most strongly correlated with the structure of microbial communities, including genera Synechococcus, Rhodopirellula, Blastopirellula, and Rubripirellula. About 50% of ppk genes obtained from the total DNA of sponge holobionts, had above 80% amino acid sequence similarities to those sequences from Synechococcus. In general, it suggested that sponges employed differentiated strategies towards the use of phosphorus in different nutrient environments and the symbiotic Synechococcus could play a key role in accumulating polyP.

scholarly journals Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Oscar N Ruiz ◽  
Derry Alvarez ◽  
Gloriene Gonzalez-Ruiz ◽  
Cesar Torres
Keyword(s):  
Polyphosphate Kinase

scholarly journals Involvement of polyphosphate kinase in virulence and stress tolerance of uropathogenic Proteus mirabilis

2015 ◽  
Vol 205 (2) ◽  
pp. 97-109 ◽  
Author(s):  
Liang Peng ◽  
Qiao Jiang ◽  
Jia-Yun Pan ◽  
Cong Deng ◽  
Jing-Yi Yu ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Proteus Mirabilis ◽  
Polyphosphate Kinase
Sign in / Sign up

Export Citation Format

Share Document