scholarly journals High throughput, colorimetric screening of microbial ester biosynthesis reveals high ethyl acetate production fromKluyveromyces marxianuson C5, C6, and C12 carbon sources

2016 ◽  
Vol 11 (10) ◽  
pp. 1274-1281 ◽  
Author(s):  
Ann-Kathrin Löbs ◽  
Jyun-Liang Lin ◽  
Megan Cook ◽  
Ian Wheeldon
Keyword(s):  
Ethyl Acetate ◽  
High Throughput ◽  
Carbon Sources ◽  
Acetate Production ◽  
Ester Biosynthesis

scholarly journals Improving Ethyl Acetate Production in Baijiu Manufacture by Wickerhamomyces anomalus and Saccharomyces cerevisiae Mixed Culture Fermentations

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Guangsen Fan ◽  
Chao Teng ◽  
Dai Xu ◽  
Zhilei Fu ◽  
Pengxiao Liu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethyl Acetate ◽  
Mixed Culture ◽  
Nutrient Competition ◽  
Acetate Production ◽  
Mixed Fermentation ◽  
Wickerhamomyces Anomalus ◽  
Flavor Perception ◽  
Positive Effect

Ethyl acetate content has strong influence on the style and quality of Baijiu. Therefore, this study investigated the effect of Saccharomyces cerevisiae Y3401 on the production of ethyl acetate by Wickerhamomyces anomalus Y3604. Analysis of cell growth showed that Y3401 influences Y3604 by nutrient competition and inhibition by metabolites, while the effect of Y3604 on Y3401 was mainly competition for nutrients. Mixed fermentation with two yeasts was found to produce more ethyl acetate than a single fermentation. The highest yield of ethyl acetate was 2.99 g/L when the inoculation ratio of Y3401:Y3604 was 1:2. Synergistic fermentation of both yeasts improved ethyl acetate production and increased the content of other flavor compounds in liquid and simulated solid-state fermentation for Baijiu. Saccharomyces cerevisiae had a positive effect on ethyl acetate production in mixed culture and provides opportunities to alter the aroma and flavor perception of Baijiu.

Ethanol and ethyl acetate production during the cider fermentation from laboratory to industrial scale

2003 ◽  
Vol 38 (10) ◽  
pp. 1451-1456 ◽  
Author(s):  
Carlos de la Roza ◽  
Adriana Laca ◽  
Luis A. Garcı́a ◽  
Mario Dı́az
Keyword(s):  
Ethyl Acetate ◽  
Industrial Scale ◽  
Acetate Production

scholarly journals From Eat to trEat: engineering the mitochondrial Eat1 enzyme for enhanced ethyl acetate production in Escherichia coli

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Aleksander J. Kruis ◽  
Anna C. Bohnenkamp ◽  
Bram Nap ◽  
Jochem Nielsen ◽  
Astrid E. Mars ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Acetate Production

scholarly journals Multilevel optimisation of anaerobic ethyl acetate production in engineered Escherichia coli

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Anna C. Bohnenkamp ◽  
Aleksander J. Kruis ◽  
Astrid E. Mars ◽  
Rene H. Wijffels ◽  
John van der Oost ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Acetate Production ◽  
Engineered Escherichia Coli ◽  
Multilevel Optimisation

scholarly journals Multi-Aspect Comparison of Ethyl Acetate Production Pathways: Reactive Distillation Process Integration and Intensification via Mechanical and Chemical Approach

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1618
Author(s):  
Branislav Šulgan ◽  
Juraj Labovský ◽  
Zuzana Labovská
Keyword(s):  
Ethyl Acetate ◽  
Case Studies ◽  
Chemical Reaction ◽  
Distillation Column ◽  
Reactive Distillation ◽  
Process Integration ◽  
Distillation Process ◽  
Acetate Production ◽  
Conventional Process ◽  
Set Up

This paper provides a multi-aspect comparison of selected methods of ethyl acetate production and shows the possibility of further reactive distillation process integration and sophisticated intensification including process stream regeneration. The production pathways were selected with respect to their practical applicability and sufficient experimental and feasibility studies already published. A total of four case studies were designed and compared: conventional process set-up (ethyl acetate is produced in a chemical reactor) is designed as a base case study; reactive distillation with a separation unit is derived from the conventional process set-up. The mechanical and chemical approach to reactive distillation process intensification and integration were assumed: reactive distillation column with a stripper and reactive distillation column with an auxiliary chemical reaction (ethylene oxide hydration). Process models were compiled in the Aspen Plus software. Complex process flowsheets of selected case studies including separation and regeneration were designed and optimized. Three different points of view were applied to evaluate the selected process benefits and drawbacks. Process energy, economy, and safety were assessed. As a result, a reactive distillation column with an auxiliary chemical reaction has been proven to be the most suitable pathway for ethyl acetate production assuming all three evaluated aspects.

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