scholarly journals Engineering at the microscale: A step towards single-cell analysis of human pluripotent stem cells

2015 ◽  
Vol 10 (10) ◽  
pp. 1511-1512
Author(s):  
Tiago G. Fernandes
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Pluripotent Stem Cells ◽  
Single Cell Analysis ◽  
Human Pluripotent Stem Cells ◽  
Cell Analysis

scholarly journals Single-Cell Analysis Reveals Partial Reactivation of X Chromosome instead of Chromosome-wide Dampening in Naive Human Pluripotent Stem Cells

2020 ◽  
Vol 14 (5) ◽  
pp. 745-754 ◽  
Author(s):  
Susmita Mandal ◽  
Deepshikha Chandel ◽  
Harman Kaur ◽  
Sudeshna Majumdar ◽  
Maniteja Arava ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
X Chromosome ◽  
Pluripotent Stem Cells ◽  
Single Cell Analysis ◽  
Human Pluripotent Stem Cells ◽  
Cell Analysis

scholarly journals Single Cell Analysis Reveals Partial Reactivation of X-chromosome Instead of Chromosome-wide Dampening in Naïve Human Pluripotent Stem Cells

2019 ◽  
Author(s):  
S Mandal ◽  
D Chandel ◽  
H Kaur ◽  
S Majumdar ◽  
M Arava ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Single Cell ◽  
X Chromosome ◽  
Pluripotent Stem Cells ◽  
Single Cell Analysis ◽  
Human Pluripotent Stem Cells ◽  
Preimplantation Embryos ◽  
X Chromosomes ◽  
Linked Gene

AbstractRecently, a unique form of X-chromosome dosage compensation has been demonstrated in human preimplantation embryos, which happens through the dampening of X-linked gene expression from both X-chromosomes. Subsequently, X-chromosome dampening has also been demonstrated in female human pluripotent stem cells (hPSCs) during the transition from primed to naïve state. However, the existence of dampened X-chromosomes remains controversial in both embryos and hPSCs. Specifically, in preimplantation embryos it has been shown that there is inactivation of X-chromosome instead of dampening. Here, we have performed allelic analysis of X-linked genes at the single cell level in hPSCs and found that there is partial reactivation of the inactive X-chromosome instead of chromosome-wide dampening upon conversion from primed to naïve state. In addition, our analysis suggests that the reduced X-linked gene expression in naïve hPSCs might be the consequence of erasure of active X-chromosome upregulation.

scholarly journals Single-Cell Transcriptome Analysis as a Promising Tool to Study Pluripotent Stem Cell Reprogramming

2021 ◽  
Vol 22 (11) ◽  
pp. 5988
Author(s):  
Hyun Kyu Kim ◽  
Tae Won Ha ◽  
Man Ryul Lee
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Cell Fate ◽  
Human Cell ◽  
Transcriptome Analysis ◽  
Single Cell Analysis ◽  
Embryonic Stem ◽  
Single Cell Level ◽  
Cell Analysis ◽  
Cell Level

Cells are the basic units of all organisms and are involved in all vital activities, such as proliferation, differentiation, senescence, and apoptosis. A human body consists of more than 30 trillion cells generated through repeated division and differentiation from a single-cell fertilized egg in a highly organized programmatic fashion. Since the recent formation of the Human Cell Atlas consortium, establishing the Human Cell Atlas at the single-cell level has been an ongoing activity with the goal of understanding the mechanisms underlying diseases and vital cellular activities at the level of the single cell. In particular, transcriptome analysis of embryonic stem cells at the single-cell level is of great importance, as these cells are responsible for determining cell fate. Here, we review single-cell analysis techniques that have been actively used in recent years, introduce the single-cell analysis studies currently in progress in pluripotent stem cells and reprogramming, and forecast future studies.

scholarly journals Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution

Cell Reports ◽  
2019 ◽  
Vol 26 (4) ◽  
pp. 815-824.e4 ◽  
Author(s):  
Tobias Messmer ◽  
Ferdinand von Meyenn ◽  
Aurora Savino ◽  
Fátima Santos ◽  
Hisham Mohammed ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells

scholarly journals Single-Cell Analysis of Hematopoietic Stem Cells

2021 ◽  
pp. 301-337
Author(s):  
Katherine H. M. Sturgess ◽  
Fernando J. Calero-Nieto ◽  
Berthold Göttgens ◽  
Nicola K. Wilson
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis ◽  
Hematopoietic Stem

scholarly journals Methods for Automated Single Cell Isolation and Sub‐Cloning of Human Pluripotent Stem Cells

2020 ◽  
Vol 55 (1) ◽  
Author(s):  
Valeria Fernandez Vallone ◽  
Narasimha Swamy Telugu ◽  
Iris Fischer ◽  
Duncan Miller ◽  
Sandra Schommer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Pluripotent Stem Cells ◽  
Cell Isolation ◽  
Human Pluripotent Stem Cells ◽  
Single Cell Isolation
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