scholarly journals Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors

2016 ◽  
Vol 11 (4) ◽  
pp. 473-486 ◽  
Author(s):  
Qasim A. Rafiq ◽  
Karen Coopman ◽  
Alvin W. Nienow ◽  
Christopher J. Hewitt
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Culture Conditions ◽  
Cell Yield ◽  
Agitated Culture

scholarly journals Influence of Discrete and Continuous Culture Conditions on Human Mesenchymal Stem Cell Lineage Choice in RGD Concentration Gradient Hydrogels

2013 ◽  
Vol 14 (9) ◽  
pp. 3047-3054 ◽  
Author(s):  
Laura A. Smith Callahan ◽  
Gina M. Policastro ◽  
Sharon L. Bernard ◽  
Erin P. Childers ◽  
Ronna Boettcher ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Continuous Culture ◽  
Concentration Gradient ◽  
Cell Lineage ◽  
Human Mesenchymal Stem Cell ◽  
Culture Conditions ◽  
Stem Cell Lineage

scholarly journals A new synthetic matrix metalloproteinase inhibitor reduces human mesenchymal stem cell adipogenesis

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0172925 ◽  
Author(s):  
Dale B. Bosco ◽  
Mark D. Roycik ◽  
Yonghao Jin ◽  
Martin A. Schwartz ◽  
Ty J. Lively ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Matrix Metalloproteinase ◽  
Human Mesenchymal Stem Cell ◽  
Matrix Metalloproteinase Inhibitor ◽  
Metalloproteinase Inhibitor ◽  
Synthetic Matrix

scholarly journals Morphological and immunocytochemical characteristics indicate the yield of early progenitors and represent a quality control for human mesenchymal stem cell culturing

2009 ◽  
Vol 214 (5) ◽  
pp. 759-767 ◽  
Author(s):  
Florian Haasters ◽  
Wolf Christian Prall ◽  
David Anz ◽  
Carole Bourquin ◽  
Christoph Pautke ◽  
...  
Keyword(s):  
Quality Control ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Cell Culturing

Labeling of human mesenchymal stem cell: Comparison between paramagnetic and superparamagnetic agents

2009 ◽  
Vol 105 (7) ◽  
pp. 07B314 ◽  
Author(s):  
Chung-Yi Yang ◽  
Ming-Fong Tai ◽  
Shin-Tai Chen ◽  
Yi-Ting Wang ◽  
Ya-Fang Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell

Biomaterials Nano Geometry for Control of Stem Cell Differentiation

2009 ◽  
Vol 1239 ◽  
Author(s):  
Karla Brammer ◽  
Seunghan Oh ◽  
Sungho Jin
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Oxide Surface ◽  
Specific Cell ◽  
Implant Surface ◽  
Multipotent Stem Cells

AbstractTwo important goals in stem cell research are to control the cell proliferation without differentiation, and also to direct the differentiation into a specific cell lineage when desired. Recent studies indicate that the nanostructures substantially influence the stem cell behavior. It is well known that mesenchymal stem cells (MSCs) are multipotent stem cells that can differentiate into stromal lineages such as adipocyte, chondrocyte, fibroblast, myocyte, and osteoblast cell types. By examining the cellular behavior of MSCs cultured in vitro on nanostructures, some understanding of the effects that the nanostructures have on the stem cell’s response has been obtained. Here we demonstrate that TiO2 nanotubes produced by anodization on Ti implant surface can regulate human mesenchymal stem cell (hMSC) differentiation towards an osteoblast lineage in the absence of osteogenic inducing factors. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion at smaller diameter levels or a specific differentiation of hMSCs into osteoblasts using only the geometric cues. Small (˜30 nm diameter) nanotubes promoted adhesion without noticeable differentiation, while larger (˜70 - 100 nm diameter) nanotubes elicited a dramatic, ˜10 fold stem cell elongation, which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for novel orthopaedics-related hMSC treatments. The fact that a guided and preferential osteogenic differentiation of stem cells can be achieved using substrate nanotopography alone without using potentially toxic, differentiation-inducing chemical agents is significant, which can be useful for future development of novel and enhanced stem cell control and therapeutic implant development.

scholarly journals Correlation Between Compositional and Mechanical Properties of Human Mesenchymal Stem Cell-Collagen Microspheres During Chondrogenic Differentiation

2011 ◽  
Vol 17 (5-6) ◽  
pp. 777-788 ◽  
Author(s):  
Chun-hei Li ◽  
Tsz-Kit Chik ◽  
Alfonso H.W. Ngan ◽  
Stanley C.H. Chan ◽  
Daisy K.Y. Shum ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cell

scholarly journals Transfer stamping of human mesenchymal stem cell patches using thermally expandable hydrogels with tunable cell-adhesive properties

Biomaterials ◽  
2015 ◽  
Vol 54 ◽  
pp. 44-54 ◽  
Author(s):  
Indong Jun ◽  
Yu Bin Lee ◽  
Yu Suk Choi ◽  
Adam J. Engler ◽  
Hansoo Park ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Human Mesenchymal Stem Cell ◽  
Adhesive Properties ◽  
Cell Adhesive
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