Heat shock protein 27 overexpression in CHO cells modulates apoptosis pathways and delays activation of caspases to improve recombinant monoclonal antibody titre in fed-batch bioreactors

2015 ◽  
Vol 10 (5) ◽  
pp. 790-800 ◽  
Author(s):  
Janice G.L. Tan ◽  
Yih Yean Lee ◽  
Tianhua Wang ◽  
Miranda G. S. Yap ◽  
Tin Wee Tan ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Antibody Titre ◽  
Cho Cells ◽  
Heat Shock Protein 27 ◽  
Fed Batch ◽  
Recombinant Monoclonal Antibody ◽  
Apoptosis Pathways ◽  
Batch Bioreactors

Principle of Express Selection of Leading Producing Clones of Monoclonal Antibodies in the Development of Stable CHO-Based Cell Lines

2019 ◽  
Vol 35 (4) ◽  
pp. 65-72
Author(s):  
V.I. Pavelko ◽  
I.A. Kirik ◽  
V.N. Bade ◽  
T.O. Malygina ◽  
R.A. Khamitov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Parallel Processing ◽  
Cell Lines ◽  
Cho Cells ◽  
Fed Batch ◽  
Cho Cell ◽  
Recombinant Monoclonal Antibody ◽  
Selection Of

Growth and productive characteristics of monoclonal cell lines based on CHO cells and producing a therapeutic protein have been monitored using the robot Ambr Tap Biosystems, which permitted to identify the leading line. Twenty four clones producing a recombinant monoclonal antibody were studied under the close to industrial conditions in a fed-batch culturing mode. The ambr®15 cell culture workstation controls 24 disposable mini bioreactors, and offers parallel processing and evaluation of multiple (24) experiments in an automated bench-top system. The volumetric productivity of 24 clones determined by ELISA was 120-450 mg /L. A protocol was shown to select a leader among producing clones for further research. producing clones, mini bioreactor; Ambr Tap Biosystems, fed-batch, monoclonal antibodies, CHO cell culture

765: Modified Expression of Heat Shock Protein 27 in Renal Cell Carcinoma

2004 ◽  
Vol 171 (4S) ◽  
pp. 203-203
Author(s):  
Cecilia Sarto ◽  
Paolo Favini ◽  
Cristina Valsecchi ◽  
Stefano Casellato ◽  
Fulvio Magni ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Heat Shock Protein 27

Protektive Wirkung von Heat Shock Protein 27 in der Magenmukosa

2005 ◽  
Vol 43 (05) ◽  
Author(s):  
M Ebert ◽  
C Schäfer ◽  
J Hoffmann ◽  
C Kubisch ◽  
G Treiber ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 27 ◽  
Protektive Wirkung

scholarly journals Involvement of Reductive Stress in the Cardiomyopathy in Transgenic Mice With Cardiac-Specific Overexpression of Heat Shock Protein 27

Hypertension ◽  
2010 ◽  
Vol 55 (6) ◽  
pp. 1412-1417 ◽  
Author(s):  
Xia Zhang ◽  
Xiaoyan Min ◽  
Chuanfu Li ◽  
Ivor J. Benjamin ◽  
Bo Qian ◽  
...  
Keyword(s):  
Heat Shock ◽  
Transgenic Mice ◽  
Heat Shock Protein ◽  
Heat Shock Protein 27 ◽  
Reductive Stress

scholarly journals Heat-shock protein 27 (Hsp27) as a target of methylglyoxal in gastrointestinal cancer

2011 ◽  
Vol 1812 (7) ◽  
pp. 769-781 ◽  
Author(s):  
Tomoko Oya-Ito ◽  
Yuji Naito ◽  
Tomohisa Takagi ◽  
Osamu Handa ◽  
Hirofumi Matsui ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Gastrointestinal Cancer ◽  
Heat Shock Protein 27
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