Salts employed in hydrophobic interaction chromatography can change protein structure - insights from protein-ligand interaction thermodynamics, circular dichroism spectroscopy and small angle X-ray scattering

2015 ◽  
Vol 10 (3) ◽  
pp. 417-426 ◽  
Author(s):  
Andras Z. Komaromy ◽  
Chadin Kulsing ◽  
Reinhard I. Boysen ◽  
Milton T. W. Hearn
Keyword(s):  
Protein Structure ◽  
Hydrophobic Interaction ◽  
Small Angle ◽  
Hydrophobic Interaction Chromatography ◽  
Ligand Interaction ◽  
X Ray ◽  
X Ray Scattering ◽  
Protein Ligand Interaction ◽  
Circular Dichroïsm ◽  
Ray Scattering

scholarly journals LabDisk for SAXS: a centrifugal microfluidic sample preparation platform for small-angle X-ray scattering

Lab on a Chip ◽  
2016 ◽  
Vol 16 (7) ◽  
pp. 1161-1170 ◽  
Author(s):  
Frank Schwemmer ◽  
Clement E. Blanchet ◽  
Alessandro Spilotros ◽  
Dominique Kosse ◽  
Steffen Zehnle ◽  
...  
Keyword(s):  
Protein Structure ◽  
Sample Preparation ◽  
Structure Analysis ◽  
Small Angle ◽  
Protein Structure Analysis ◽  
X Ray ◽  
X Ray Scattering ◽  
Synchrotron Beamlines ◽  
Ray Scattering

We present a centrifugal microfluidic LabDisk for protein structure analysis via small-angle X-ray scattering (SAXS) on synchrotron beamlines.

scholarly journals Study of DNA-spermine interactions by use of small-angle and wide-angle X-ray scattering and circular dichroism

1978 ◽  
Vol 5 (10) ◽  
pp. 3801-3810 ◽  
Author(s):  
Hilde Damaschun ◽  
G. Damaschun ◽  
M. Becker ◽  
E. Buder ◽  
R. Misselwitz ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Small Angle ◽  
Wide Angle ◽  
X Ray ◽  
X Ray Scattering ◽  
Circular Dichroïsm ◽  
Ray Scattering

Structural Insights into the β-Xylosidase fromTrichoderma reeseiObtained by Synchrotron Small-Angle X-ray Scattering and Circular Dichroism Spectroscopy†

Biochemistry ◽  
2005 ◽  
Vol 44 (47) ◽  
pp. 15578-15584 ◽  
Author(s):  
Adriana L. Rojas ◽  
Hannes Fischer ◽  
Elena V. Eneiskaya ◽  
Anna A. Kulminskaya ◽  
Konstantin A. Shabalin ◽  
...  
Keyword(s):  
Circular Dichroism ◽  
Small Angle ◽  
Circular Dichroism Spectroscopy ◽  
X Ray ◽  
X Ray Scattering ◽  
Circular Dichroïsm ◽  
Structural Insights ◽  
Ray Scattering
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