Bioactive nanoparticles stimulate bone tissue formation in bioprinted three-dimensional scaffold and human mesenchymal stem cells

2014 ◽  
Vol 9 (10) ◽  
pp. 1304-1311 ◽  
Author(s):  
Guifang Gao ◽  
Arndt F. Schilling ◽  
Tomo Yonezawa ◽  
Jiang Wang ◽  
Guohao Dai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Tissue Formation ◽  
Bone Tissue Formation

scholarly journals 3D Printed SiOC(N) Ceramic Scaffolds for Bone Tissue Regeneration: Improved Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

2021 ◽  
Vol 22 (24) ◽  
pp. 13676
Author(s):  
Yuejiao Yang ◽  
Apoorv Kulkarni ◽  
Gian Domenico Soraru ◽  
Joshua M. Pearce ◽  
Antonella Motta
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
3D Printing ◽  
Pore Size ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Low Cost ◽  
Human Mesenchymal Stem Cells ◽  
Bone Tissue Regeneration ◽  
Gene Expressions

Bone tissue engineering has developed significantly in recent years as there has been increasing demand for bone substitutes due to trauma, cancer, arthritis, and infections. The scaffolds for bone regeneration need to be mechanically stable and have a 3D architecture with interconnected pores. With the advances in additive manufacturing technology, these requirements can be fulfilled by 3D printing scaffolds with controlled geometry and porosity using a low-cost multistep process. The scaffolds, however, must also be bioactive to promote the environment for the cells to regenerate into bone tissue. To determine if a low-cost 3D printing method for bespoke SiOC(N) porous structures can regenerate bone, these structures were tested for osteointegration potential by using human mesenchymal stem cells (hMSCs). This includes checking the general biocompatibilities under the osteogenic differentiation environment (cell proliferation and metabolism). Moreover, cell morphology was observed by confocal microscopy, and gene expressions on typical osteogenic markers at different stages for bone formation were determined by real-time PCR. The results of the study showed the pore size of the scaffolds had a significant impact on differentiation. A certain range of pore size could stimulate osteogenic differentiation, thus promoting bone regrowth and regeneration.

scholarly journals Three-dimensional carbon nanotube scaffolds for long-term maintenance and expansion of human mesenchymal stem cells

2017 ◽  
Vol 105 (7) ◽  
pp. 1927-1939 ◽  
Author(s):  
Gaurav Lalwani ◽  
Michael D'agati ◽  
Anu Gopalan ◽  
Sunny C. Patel ◽  
Yahfi Talukdar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Carbon Nanotube ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Term Maintenance

scholarly journals Three-dimensional graphene foams promote osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽  
2013 ◽  
Vol 5 (10) ◽  
pp. 4171 ◽  
Author(s):  
Spencer W. Crowder ◽  
Dhiraj Prasai ◽  
Rutwik Rath ◽  
Daniel A. Balikov ◽  
Hojae Bae ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Graphene Foams
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