scholarly journals Phosphorylation of silk fibroins improves the cytocompatibility of silk fibroin derived materials: A platform for the production of tuneable material

2014 ◽  
Vol 9 (10) ◽  
pp. 1267-1278 ◽  
Author(s):  
Vadim Volkov ◽  
Andreia Vasconcelos ◽  
Marisa P. Sárria ◽  
Andreia C. Gomes ◽  
Artur Cavaco-Paulo
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroins

Recombinant silk fibroin incorporated cell-adhesive sequences produced by transgenic silkworm as a possible candidate for use in vascular graft

2014 ◽  
Vol 2 (42) ◽  
pp. 7375-7383 ◽  
Author(s):  
Tetsuo Asakura ◽  
Makoto Isozaki ◽  
Toshiki Saotome ◽  
Ken-ichiro Tatematsu ◽  
Hideki Sezutsu ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Vascular Graft ◽  
Transgenic Silkworm ◽  
Cell Adhesive ◽  
Silk Fibroins

Transgenic silk fibroins that incorporated the laminin sequence were prepared. The adhesive activities tend to increase in the TG silk fibroins relative to WT silk fibroins.

scholarly journals Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Huijing Zhao ◽  
Siyong Xiong ◽  
Mingzhong Li ◽  
Qiang Zhang ◽  
Guiyang Liu
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Gelation Time ◽  
X Ray Diffraction ◽  
Antheraea Pernyi ◽  
X Ray ◽  
Silk Fibroins ◽  
Ft Ir ◽  
Additional Formation ◽  
Ir And Raman

Silk fibroin (SF) hydrogels were obtained from both domestic (Bombyx mori) and wild (Antheraea pernyi) silkworms from aqueous silk fibroin solutions at room temperature. The gelation time of theAntheraea pernyi(A. pernyi) SF solution was significantly shorter than that of theBombyx mori(B. mori) SF solution. The secondary structures of the two kinds of hydrogels were also compared. In order to further reduce the gelation time, various amounts of polyethylene glycol (PEG) were blended with the silk fibroins ofA. pernyiandB. mori. The gelation time of bothA. pernyiSF andB. moriSF decreased with the increased amount of PEG. After freeze-drying, the hydrogels were characterized through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Results showed that the addition of polyalcohol did not change the main secondary structure of the hydrogels. However, the addition of polyalcohol did reduce the gelation time and triggered additional formation ofβ-sheets.

Ultrasonication-Induced Rapid Gelation of Wild Silkworm Silk Fibroins

2013 ◽  
Vol 634-638 ◽  
pp. 1165-1169 ◽  
Author(s):  
Gui Yang Liu ◽  
Si Yong Xiong ◽  
Ren Chuan You ◽  
Ling Shuang Wang ◽  
Ming Zhong Li
Keyword(s):  
Secondary Structure ◽  
Silk Fibroin ◽  
Gelation Time ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Freeze Dried ◽  
Silk Fibroins ◽  
Wild Silkworm ◽  
Α Helix ◽  
Silkworm Silk

Silk fibroin （SF） hydrogels of the wild silkworm species Antheraea pernyi and Antheraea yamamai were obtained from aqueous SF solutions at room temperature. Both A. pernyi and A. yamamai solutions were slow to gelate. Hydrogels of the two species of wild silkworm were obtained rapidly following ultrasonicaton at 400–500 W. The secondary structure of the freeze-dried SF hydrogels was measured by X-ray diffraction and Fourier transform infrared spectroscopy. Ultrasonication did not change the main secondary structure of the hydrogels, but it accelerated the structural transformation of silk fibroin molecules from random coil or α helix to β sheet and reduced the gelation time.

Introduction of VEGF or RGD sequences improves revascularization properties of Bombyx mori silk fibroin produced by transgenic silkworm

2015 ◽  
Vol 3 (35) ◽  
pp. 7109-7116 ◽  
Author(s):  
Toshiki Saotome ◽  
Haruki Hayashi ◽  
Ryo Tanaka ◽  
Atsushi Kinugasa ◽  
Shouji Uesugi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Platelet Adhesion ◽  
Transgenic Silkworm ◽  
Silk Fibroins ◽  
Bombyx Mori Silk

Transgenic silk fibroins incorporated the VEGF and RGD were prepared. The VEGF SF showed lower platelet adhesion than the RGD SF and WT SF. An in vivo implantation study supported these in vitro results.

Study on the Effect of Sericin Content on Solubility of Silk Fibroin and the Properties of Silk Fibroin Membranes

2013 ◽  
Vol 319 ◽  
pp. 165-168
Author(s):  
Yu Chun Jiang ◽  
Yuan Song Zhang
Keyword(s):  
Silk Fibroin ◽  
Silk Fibroins ◽  
Silkworm Cocoon ◽  
Different Content ◽  
Better Than

In this paper silkworm cocoon was dissolved at different time by degumming agents of NaHCO3 to obtain silk fibroins with different content of sericin. Then the silk fibroins was dissolved to get the silk fibroin membranes. The results indicated that the content of sericin had certain effect on silk solubility. As the content of sericin reduced, the dissolution speed was faster and the silk solubility was greater. The properties of the silk fibroin membranes with some sericin were much better than those degummed completely.

scholarly journals A Comparative Study on the Dissolution of Argema mimosae Silk Fibroin and Fabrication of Films and Nanofibers

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 549
Author(s):  
Hlobsile Kgomo ◽  
Somandla Ncube ◽  
Vimbai Mhuka ◽  
Temesgen Girma Kebede ◽  
Simiso Dube ◽  
...  
Keyword(s):  
Formic Acid ◽  
Silk Fibroin ◽  
Influence Of Temperature ◽  
Regenerated Silk Fibroin ◽  
Silk Fibroins ◽  
Contour Plots ◽  
Wild Silkworm ◽  
Β Sheets ◽  
Β Sheet ◽  
Central Composite

Limited studies have been done on silk fibroins of wild silkworm species owing to their relative insolubility in many solvents. In this study, the solubility of Argema mimosae wild silk fibroin in different salts (LiBr, LiCl, Ca(NO3)2, and CaCl2) dissolved in formic acid under varying temperatures was investigated. The dissolution conditions under which the solubility was optimum were optimized using a central composite design approach. The optimum range for solvation of the fibroin were visualized using contour plots. The influence of temperature and salt concentration were found to significantly influence the solvation of the fibroin. Following the successful dissolution of the fibroin, the regenerated silk fibroin solutions were cast to obtain water insoluble films which were used in investigating optimum electrospinning conditions. Average nanofiber diameters in the 110–141 nm range were obtained under optimum electrospinning conditions. The silk forms were characterized using the FTIR, TGA, XRD, and SEM to understand their properties. The investigations revealed that formic acid—salt solvents were effective in the solvation of the wild silk fibroin. Some of the dissolution conditions induced mild effects on the silk fibroin while others were harsh. Furthermore, processing to nanofibers resulted in the degradation of the β-sheets producing nanofibers rich in α-helices. However, post-treatment using methanol and water vapor were effective in restoring β-sheet crystallinity.

Optimization Preparation and Emulsifying Properties of Carboxymethyl Silk Fibroin

2013 ◽  
Vol 30 (1) ◽  
pp. 73
Author(s):  
Wei JING ◽  
Jun-sheng LI ◽  
Guo-xia HUANG ◽  
Liu-juan YAN ◽  
Chun-hua LAI
Keyword(s):  
Silk Fibroin ◽  
Emulsifying Properties
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