Aqueous biphasic cancer cell migration assay enables robust, high-throughput screening of anti-cancer compounds

2013 ◽  
Vol 9 (3) ◽  
pp. 426-434 ◽  
Author(s):  
Stephanie Lemmo ◽  
Samila Nasrollahi ◽  
Hossein Tavana
Keyword(s):  
Cell Migration ◽  
Cancer Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cancer Cell Migration ◽  
Cell Migration Assay ◽  
Migration Assay ◽  
Anti Cancer ◽  
Aqueous Biphasic

scholarly journals Quantification of cancer cell migration with an integrated experimental-computational pipeline

2017 ◽  
Author(s):  
Edwin F. Juarez ◽  
Carolina Garri ◽  
Ahmadreza Ghaffarizadeh ◽  
Paul Macklin ◽  
Kian Kani
Keyword(s):  
Breast Cancer ◽  
Cell Migration ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cancer Cell Migration ◽  
Computational Pipeline ◽  
Migration Assay ◽  
User Friendly ◽  
Migration Of Cells

AbstractWe describe an integrated experimental-computational pipeline for quantifying cell migration in vitro. This pipeline is robust to image noise, open source, and user friendly. The experimental component uses the Oris cell migration assay (Platypus Technologies) to create migration regions. The computational component of the pipeline creates masks in Matlab (MathWorks) to cell-covered regions, uses a genetic algorithm to automatically select the migration region, and outputs a metric to quantify the migration of cells. In this work we demonstrate the utility of our pipeline by quantifying the effects of a drug (Taxol) and of the secreted Anterior Gradient 2 (sAGR2) protein in the migration of MDA-MB-231 cells (a breast cancer cell line). In particular, we show that blocking sAGR2 reduces migration of MDA-MB-231 cells.

scholarly journals Evaluation of a high-throughput in vitro endothelial cell migration assay for the assessment of nicotine and tobacco delivery products

2020 ◽  
Vol 334 ◽  
pp. 110-116
Author(s):  
Emma Bishop ◽  
Damien Breheny ◽  
Katherine Hewitt ◽  
Mark Taylor ◽  
Tomasz Jaunky ◽  
...  
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
High Throughput ◽  
Endothelial Cell Migration ◽  
Cell Migration Assay ◽  
Migration Assay
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