Stimuli-Responsive magnetic nanoparticles for monoclonal antibody purification

2013 ◽  
Vol 8 (6) ◽  
pp. 709-717 ◽  
Author(s):  
Luís Borlido ◽  
Leila Moura ◽  
Ana M. Azevedo ◽  
Ana C. A. Roque ◽  
Maria R. Aires-Barros ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles ◽  
Stimuli Responsive ◽  
Antibody Purification

Preparation of functionalized Fe3O4@SiO2 magnetic nanoparticles for monoclonal antibody purification

2016 ◽  
Vol 32 (6) ◽  
pp. 889-894 ◽  
Author(s):  
Xuemei Hou ◽  
Changjie Zhao ◽  
Yanlong Tian ◽  
Shuliang Dou ◽  
Xiang Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles ◽  
Antibody Purification

Immunofluorescence Assay Using Monoclonal and Polyclonal Antibodies for Detection of Staphylococcal Enterotoxins A in Milk

2019 ◽  
Vol 13 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Tzonka Godjevargova ◽  
Zlatina Becheva ◽  
Yavor Ivanov ◽  
Andrey Tchorbanov
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Food Poisoning ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin A ◽  
Fluorescence Immunoassay ◽  
Magnetic Separator ◽  
Milk Samples

Objectives: Staphylococcus aureus is a Gram-positive microorganism. S. aureus can grow in various foods and cause food poisoning by secreting enterotoxins. The most common enterotoxins involved in food poisoning are staphylococcal enterotoxin A and staphylococcal enterotoxin B, but Staphylococcal Enterotoxin A (SEA) is predominant. The main types of food contaminated with SEs are meat and meat products, poultry and eggs, milk and dairy products. The aim of this study was to develop a rapid and sensitive fluorescence immunoassay for detection of staphylococcal enterotoxin A in milk. Methods: Monoclonal and polyclonal antibodies for SEA were produced and characterized. Competitive fluorescence immunoassay based on Magnetic Nanoparticles (MNPs) was performed and optimized. MNPs were used as a solid carrier of the antibodies. The first step of the assay was immunoreaction between the immobilized antibody onto MNPs and SEA in milk sample. Then the fluorescein-SEA conjugate was added to the sample. Thus, competitive immunoreaction between MNP-mAb/MNP-pAb with SEA and SEA-FITC was performed. These immuno-complexes were separated by a magnetic separator and the obtained supernatants were analyzed. The fluorescent signal from the excess of conjugated SEA was proportional to the SEA contained in the milk. The assay duration was only 30 min. Results: The fluorescence immunoassays performed with polyclonal antibody had linear ranges from 5 pg/mL to 100 ng/mL SEA in a buffer, and from 50 pg/mL to 50 ng/mL SEA in spiked milk samples. While the same assays performed with monoclonal antibody had linear ranges from 1 pg/mL to 20 ng/mL SEA in buffer, and from 10 pg/mL to 10 ng/mL SEA in spiked milk samples. The detection limits of the developed immunoassays performed in milk were: 48 pg/mL with polyclonal antibody and 9 pg/mL with monoclonal antibody. Conclusion: A rapid and sensitive fluorescence immunoassay based on magnetic nanoparticles with a polyclonal and monoclonal antibody for determination of staphylococcal enterotoxin A in milk was developed.

scholarly journals Monoclonal antibody conjugated magnetic nanoparticles could target MUC-1-positive cellsin vitrobut notin vivo

2014 ◽  
Vol 10 (3) ◽  
pp. 225-236 ◽  
Author(s):  
Saeed Shanehsazzadeh ◽  
Cordula Gruettner ◽  
Afsaneh Lahooti ◽  
Morteza Mahmoudi ◽  
Barry J. Allen ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Magnetic Nanoparticles

scholarly journals Microgels and Nanoparticles: Where Micro and Nano Go Hand in Hand

2015 ◽  
Vol 229 (1-2) ◽  
Author(s):  
Beatriz H. Juarez ◽  
Luis M. Liz-Marzán
Keyword(s):  
Magnetic Nanoparticles ◽  
Optical Tweezers ◽  
Hybrid System ◽  
Inorganic Nanoparticles ◽  
Stimuli Responsive ◽  
Single Particles ◽  
Nanoscale Systems ◽  
Different Types ◽  
Potential Applications ◽  
Very High

AbstractThe integration of different types of materials in a single hybrid system allows the combination of multiple functionalities, which can even be used in conjunction with each other. This strategy has been exploited in nanoscale systems for the creation of so-called smart nanomaterials. Within this category, the combination of inorganic nanoparticles with stimuli-responsive microgels is of very high interest because of the wide variety of potential applications. We present here a short overview of this type of materials in which the nano- and micro-scales get nicely integrated, with a great potential to expand the range of technological applications. We focus mainly on the integration of metal nanoparticles, either by themselves or in combination with semiconductor and magnetic nanoparticles. Various examples of the synergic properties that can be obtained are described, as well as the possibility to extract useful information when optical tweezers are used to manipulate single particles. We expect that this review will stimulate additional research in this field.

A safe, effective, and facility compatible cleaning in place procedure for affinity resin in large-scale monoclonal antibody purification

2013 ◽  
Vol 1308 ◽  
pp. 86-95 ◽  
Author(s):  
Lu Wang ◽  
Jill Dembecki ◽  
Neil E. Jaffe ◽  
Brian W. O’Mara ◽  
Hui Cai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Large Scale ◽  
Antibody Purification ◽  
Affinity Resin

Integrated Polishing Steps for Monoclonal Antibody Purification

2009 ◽  
pp. 145-167 ◽  
Author(s):  
Sanchayita Ghose ◽  
Mi Jin ◽  
Jia Liu ◽  
John Hickey
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Purification

INTEGRATED POLISHING STEPS FOR MONOCLONAL ANTIBODY PURIFICATION

2017 ◽  
pp. 303-323
Author(s):  
Sanchayita Ghose ◽  
Mi Jin ◽  
Jia Liu ◽  
John Hickey ◽  
Steven Lee
Keyword(s):  
Monoclonal Antibody ◽  
Antibody Purification
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