scholarly journals Modular bioreactor for primary human hepatocyte culture: Medium flow stimulates expression and activity of detoxification genes

2011 ◽  
Vol 6 (5) ◽  
pp. 554-564 ◽  
Author(s):  
Bruna Vinci ◽  
Cédric Duret ◽  
Sylvie Klieber ◽  
Sabine Gerbal-Chaloin ◽  
Antonio Sa-Cunha ◽  
...  
Keyword(s):  
Culture Medium ◽  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocyte ◽  
Medium Flow ◽  
Expression And Activity

scholarly journals Assessing the compatibility of primary human hepatocyte culture within porous silk sponges

RSC Advances ◽  
2020 ◽  
Vol 10 (62) ◽  
pp. 37662-37674
Author(s):  
David A. Kukla ◽  
Whitney L. Stoppel ◽  
David L. Kaplan ◽  
Salman R. Khetani
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Matrix Proteins ◽  
Human Hepatocytes ◽  
Human Hepatocyte ◽  
Matrix Proteins ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocytes ◽  
Primary Human Hepatocyte ◽  
Silk Scaffolds ◽  
Parenchymal Cells

Porous silk scaffolds hybridized with extracellular matrix proteins are useful for culture of primary human hepatocytes ± supportive non-parenchymal cells.

scholarly journals Mechanistic evaluation of primary human hepatocyte culture using global proteomic analysis reveals a selective dedifferentiation profile

2016 ◽  
Vol 91 (1) ◽  
pp. 439-452 ◽  
Author(s):  
James A. Heslop ◽  
Cliff Rowe ◽  
Joanne Walsh ◽  
Rowena Sison-Young ◽  
Roz Jenkins ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocyte

Influence of commonly used clinical antidotes on antioxidant systems in human hepatocyte culture intoxicated with α-amanitin

2010 ◽  
Vol 30 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Jan Magdalan ◽  
Aleksandra Piotrowska ◽  
Agnieszka Gomułkiewicz ◽  
Tomasz Sozański ◽  
Adam Szeląg ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Culture Medium ◽  
Antioxidant Properties ◽  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Antioxidant Systems ◽  
Effective Protection ◽  
Sod Activity ◽  
Severe Hepatotoxicity ◽  
Primary Mechanism

α-Amanitin (α-AMA) is the main toxin of Amanita phalloides and its subspecies (A. virosa and A. verna). The primary mechanism of α-AMA toxicity is associated with protein synthesis blocking in hepatocytes. Additionally, α-AMA exhibits prooxidant properties that may contribute to its severe hepatotoxicity. The aim of the present study was to assess the effect of α-AMA on lipid peroxidation and the activities of superoxide dismutase (SOD) and catalase (CAT) in human hepatocyte culture. The effects of benzylpenicillin (BPCN), N-acetyl-L-cysteine (ACC), and silibinin (SIL) on SOD and CAT activities and on lipid peroxidation in human hepatocyte culture intoxicated with α-AMA were also examined. In human hepatocyte culture, 48-hour exposure to α-AMA at a 2-μM concentration caused an increase in SOD activity, a reduction of CAT activity, and a significant increase in lipid peroxidation. Changes in SOD and CAT activity caused by α-AMA could probably enhance lipid peroxidation by increased generation of hydrogen peroxide combined with reduced detoxification of that oxygen radical. The addition of antidotes (ACC or SIL) to the culture medium provided more effective protection against lipid peroxidation in human hepatocytes intoxicated with α-AMA than the addition of BPCN, possessing no antioxidant properties.

Reexpression of cytokeratin-19 in a primary human hepatocyte culture

1998 ◽  
Vol 126 (1) ◽  
pp. 748-750
Author(s):  
A. P. Kiyasov ◽  
A. A. Gumerova ◽  
S. V. Petrov ◽  
S. H. Yap
Keyword(s):  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Cytokeratin 19 ◽  
Primary Human Hepatocyte

Primary Human Hepatocyte Culture for HCV Study

2009 ◽  
pp. 373-382 ◽  
Author(s):  
Haizhen Zhu ◽  
John Elyar ◽  
Robin Foss ◽  
Alan Hemming ◽  
Eric Hall ◽  
...  
Keyword(s):  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocyte

Alginate-encapsulated primary human hepatocyte culture system to increase the accuracy of the predicted in vivo half-life of low clearance compounds

2018 ◽  
Vol 33 (1) ◽  
pp. S69-S70
Author(s):  
Miyoung Yoon ◽  
Martin B. Phillips ◽  
Jeffrey R. Enders ◽  
Pergentino Balbuena-Venancio ◽  
David Billings ◽  
...  
Keyword(s):  
Half Life ◽  
Culture System ◽  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocyte

Primary Human Hepatocyte Culture for the Study of HCV

2003 ◽  
pp. 495-500
Author(s):  
John F. O'Connell ◽  
Stuart Cox ◽  
Peter Buontempo ◽  
Angela Skelton ◽  
Liubomir A. Pisarov ◽  
...  
Keyword(s):  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Primary Human Hepatocyte
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