An efficient and rapid transgenic pollen screening and detection method using flow cytometry

2010 ◽  
Vol 6 (1) ◽  
pp. 118-123 ◽  
Author(s):  
Hong S. Moon ◽  
Shigetoshi Eda ◽  
Arnold M. Saxton ◽  
David W. Ow ◽  
C. Neal Stewart
Keyword(s):  
Flow Cytometry ◽  
Detection Method ◽  
Transgenic Pollen

scholarly journals A Kernel‐Based Change Detection Method to Map Shifts in Phytoplankton Communities Measured by Flow Cytometry

2021 ◽  
Author(s):  
Corinne Jones ◽  
Sophie Clayton ◽  
François Ribalet ◽  
E. Virginia Armbrust ◽  
Zaid Harchaoui
Keyword(s):  
Flow Cytometry ◽  
Change Detection ◽  
Detection Method ◽  
Phytoplankton Communities

A rapid detection method using flow cytometry to monitor the risk of Legionella in bath water

2011 ◽  
Vol 86 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Toshitsugu Taguri ◽  
Yasunori Oda ◽  
Kanji Sugiyama ◽  
Toru Nishikawa ◽  
Takuro Endo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Rapid Detection ◽  
Detection Method ◽  
Bath Water

scholarly journals Establishing Novel Bacteriophage Detection Method Based on Imagining Flow Cytometry

2019 ◽  
Vol 252 ◽  
pp. 042116
Author(s):  
Jiayi Yang ◽  
Sizhang Liu ◽  
Zhiwei Sui ◽  
Jing Wang ◽  
Chunyan Niu
Keyword(s):  
Flow Cytometry ◽  
Detection Method

Multi-detection method for five common microalgal toxins based on the use of microspheres coupled to a flow-cytometry system

2014 ◽  
Vol 850 ◽  
pp. 57-64 ◽  
Author(s):  
María Fraga ◽  
Natalia Vilariño ◽  
M. Carmen Louzao ◽  
Laura P. Rodríguez ◽  
Amparo Alfonso ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Detection Method

Binding of Heparin to Human Leukocytes

1994 ◽  
Vol 14 (01) ◽  
pp. 16-24 ◽  
Author(s):  
R. Malsch ◽  
L. Piazolo ◽  
D. L. Heene ◽  
J. Harenberg
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Intensity ◽  
Biological Function ◽  
Detection Method ◽  
Biological Activities ◽  
Anticoagulant Activity ◽  
Sulfated Polysaccharides ◽  
Chromogenic Substrate ◽  
Leukocyte Populations ◽  
Monocytes And Lymphocytes

SummaryNon-anticoagulant actions of heparins and related sulfated polysaccharides in thrombosis, atherosclerosis and inflammation are currently under investigation. To analyze the involvement of leukocytes in this mechanism, a fluorescein labeled low molecular mass heparin-tyramine has been prepared (LMMH-tyr-Fitc) to investigate the binding of heparin and other sulfated polysaccharides to granulocytes, monocytes, and lymphocytes. The fluorescence intensity on leukocytes was quantified using flow cytometry as detection method. LMMH-tyr-Fitc bound dose-dependently to all three leukocyte populations. Phycoerythrin-labeled CD-antibodies identified the specificity of the binding of LMMH-tyr-Fitc to lymphocytes, monocytes, and granulocytes. Unfractionated heparin and LMM-heparin displaced LMMH-tyr-Fitc dose-dependently from granulocytes, monocytes, and lymphocytes and were more effective compared with dextransulfate. Heparin, LMM-heparin and LMMH-tyr-Fitc bound to leukocytes inhibited factors Xa activity in the S2222 chromogenic substrate assay. The data indicate that negatively charged polysaccharides bind to the surface of granulocytes, monocytes and lymphocytes and that binding is in part depending on the number of negatively charged groups of glycosaminoglycans. After binding to the surface of leukocytes heparin exerts still anticoagulant activity indicating its intact biological function. The binding of heparins to leukocytes may significantly contribute to the antithrombotic and to other biological activities.

Myeloperoxidase Expression in B-Lymphoblastic Leukemia by Immunohistochemistry as a Diagnostic Confounder

2020 ◽  
Vol 154 (Supplement_1) ◽  
pp. S1-S1
Author(s):  
Jing Du ◽  
Amanda Moklebust ◽  
Sindhu Cherian ◽  
Kerstin Edlefsen ◽  
Jonathan R Fromm ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Acute Leukemia ◽  
Detection Method ◽  
Detection System ◽  
Lymphoblastic Leukemia ◽  
Detailed Comparison ◽  
Staining Intensity ◽  
Concurrent Flow ◽  
Myeloid Antigens ◽  
B Lineage

Abstract Background B-lymphoblastic leukemia (BLL) is a B lineage neoplasm that expresses typical immature B cell markers, but may also express myeloid-associated antigens. Myeloperoxidase (MPO) is a heme-containing peroxidase, which has been used as the single most specific myeloid marker when assigning lineage to acute leukemia. MPO positivity by immunohistochemistry (IHC) in BLL has been historically described in a subset of patients; however, further detailed comparison of IHC to flow cytometry and IHC methodology is described herein. Design The University of Washington pathology database was searched for new or relapsed adult BLL from 2011–2019. Cases with blasts >30% of marrow cellularity by morphology were selected. MPO IHC was performed using the Dako, polyclonal antibody (rabbit) on a Ventana instrument with streptavidin-biotin (SB) detection method. MPO IHC SB positive cases were also stained using the same antibody and platform, but a different detection method, multimer-optiview (MO). MPO IHC was called positive when >10% of neoplastic blasts showed expression. MPO positive blast percentage, staining intensity and pattern were assessed. Intensity: 0=negative, 1=mild, 2=moderate, 3=bright/same level as myeloids. Cytoplasmic pattern: homogenous or granular. Concurrent flow cytometry results and cytogenetic and/or molecular studies were reviewed. Results 35 cases were identified. Positive MPO IHC expression was present in 7/35 cases by SB method, with the percentage of MPO positive blasts ranging from 20–90% (majority >70%), all ranged from 1 - 2+ intensity and most (5/7) were homogenous pattern. 4/5 MPO SB positive cases were negative by MO detection method. MPO evaluation by flow cytometry was negative in 3 of 3 cases and myeloid associated antigens were negative or low on a subset. 2/8 BLL, BCR-ABL1 cases were MPO IHC positive by SB. Conclusion MPO staining by IHC in BLL is present in 17% of cases, often present in the majority of blasts, and positive using the SB detection system. This aberrant staining can be negated in most cases with the MO detection system. MPO expression by flow cytometry in MPO IHC SB positive cases were negative (3/3) and were low to absent for other myeloid antigens. We agree with prior studies that MPO IHC using the SB method can be a confounder for lineage assignment in acute leukemia.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

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