One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase

Ramon Kranaster; Matthias Drum; Nicole Engel; Manfred Weidmann; Frank T. Hufert; Andreas Marx

doi:10.1002/biot.200900200

One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase

Biotechnology Journal ◽

10.1002/biot.200900200 ◽

2010 ◽

Vol 5 (2) ◽

pp. 224-231 ◽

Cited By ~ 21

Author(s):

Ramon Kranaster ◽

Matthias Drum ◽

Nicole Engel ◽

Manfred Weidmann ◽

Frank T. Hufert ◽

...

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Pathogen Detection ◽

Reverse Transcriptase Activity ◽

Thermus Aquaticus ◽

Transcriptase Activity ◽

One Step

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High sensitive RNA detection by one-step RT-PCR using the genetically engineered variant of DNA polymerase with reverse transcriptase activity from hyperthermophilies

Journal of Bioscience and Bioengineering ◽

10.1016/j.jbiosc.2017.10.004 ◽

2018 ◽

Vol 125 (3) ◽

pp. 275-281 ◽

Cited By ~ 5

Author(s):

Hiroyuki Okano ◽

Misato Baba ◽

Katsuhiro Kawato ◽

Ryota Hidese ◽

Itaru Yanagihara ◽

...

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Reverse Transcriptase Activity ◽

Genetically Engineered ◽

Rt Pcr ◽

Rna Detection ◽

Transcriptase Activity ◽

One Step

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Identification of Thermus aquaticus DNA polymerase variants with increased mismatch discrimination and reverse transcriptase activity from a smart enzyme mutant library

Scientific Reports ◽

10.1038/s41598-018-37233-y ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Govindan Raghunathan ◽

Andreas Marx

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Reverse Transcriptase Activity ◽

Mutant Library ◽

Thermus Aquaticus ◽

Transcriptase Activity ◽

Mismatch Discrimination

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High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2017.04.030 ◽

2017 ◽

Vol 487 (1) ◽

pp. 128-133 ◽

Cited By ~ 4

Author(s):

Hiroyuki Okano ◽

Misato Baba ◽

Tomomi Yamasaki ◽

Ryota Hidese ◽

Shinsuke Fujiwara ◽

...

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Rna Helicase ◽

Reverse Transcriptase Activity ◽

Rt Pcr ◽

Transcriptase Activity ◽

One Step

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Innate Reverse Transcriptase Activity of DNA Polymerase for Isothermal RNA Direct Detection

Journal of the American Chemical Society ◽

10.1021/jacs.5b08144 ◽

2015 ◽

Vol 137 (43) ◽

pp. 13804-13806 ◽

Cited By ~ 49

Author(s):

Chao Shi ◽

Xiaotong Shen ◽

Shuyan Niu ◽

Cuiping Ma

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Direct Detection ◽

Reverse Transcriptase Activity ◽

Transcriptase Activity

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Directed Evolution of DNA Polymerase, RNA Polymerase and Reverse Transcriptase Activity in a Single Polypeptide

Journal of Molecular Biology ◽

10.1016/j.jmb.2006.06.050 ◽

2006 ◽

Vol 361 (3) ◽

pp. 537-550 ◽

Cited By ~ 67

Author(s):

Jennifer L. Ong ◽

David Loakes ◽

Szymon Jaroslawski ◽

Kathleen Too ◽

Philipp Holliger

Keyword(s):

Rna Polymerase ◽

Reverse Transcriptase ◽

Directed Evolution ◽

Dna Polymerase ◽

Reverse Transcriptase Activity ◽

Transcriptase Activity ◽

Single Polypeptide

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Reverse Transcriptase Activity of Hepatitis B Virus (HBV) DNA Polymerase within Core Capsid: Interaction with Deoxynucleoside Triphosphates and Anti-HBV l-Deoxynucleoside Analog Triphosphates

Molecular Pharmacology ◽

10.1124/mol.65.2.400 ◽

2004 ◽

Vol 65 (2) ◽

pp. 400-406 ◽

Cited By ~ 5

Author(s):

Wing Lam ◽

Ying Li ◽

Jieh-Yuan Liou ◽

Ginger E. Dutschman ◽

Yung-chi Cheng

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Reverse Transcriptase ◽

Dna Polymerase ◽

Reverse Transcriptase Activity ◽

Hbv Dna ◽

Transcriptase Activity ◽

B Virus

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Limited reverse transcriptase activity of phi29 DNA polymerase

Nucleic Acids Research ◽

10.1093/nar/gky190 ◽

2018 ◽

Vol 46 (7) ◽

pp. 3625-3632 ◽

Cited By ~ 5

Author(s):

Tomasz Krzywkowski ◽

Malte Kühnemund ◽

Di Wu ◽

Mats Nilsson

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Reverse Transcriptase Activity ◽

Transcriptase Activity ◽

Phi29 Dna Polymerase

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Evolving Thermostable Reverse Transcriptase Activity in a DNA Polymerase Scaffold

Angewandte Chemie International Edition ◽

10.1002/anie.200602772 ◽

2006 ◽

Vol 45 (45) ◽

pp. 7633-7635 ◽

Cited By ~ 46

Author(s):

Katharina B. M. Sauter ◽

Andreas Marx

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Reverse Transcriptase Activity ◽

Transcriptase Activity

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From The Cover: Reverse transcriptase activity innate to DNA polymerase I and DNA topoisomerase I proteins of Streptomyces telomere complex

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.0404386101 ◽

2004 ◽

Vol 101 (40) ◽

pp. 14361-14366 ◽

Cited By ~ 25

Author(s):

K. Bao ◽

S. N. Cohen

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Topoisomerase I ◽

Reverse Transcriptase Activity ◽

Dna Topoisomerase I ◽

Dna Polymerase I ◽

Dna Topoisomerase ◽

Transcriptase Activity ◽

Polymerase I

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Human DNA polymerase η has reverse transcriptase activity in cellular environments

Journal of Biological Chemistry ◽

10.1074/jbc.ra119.007925 ◽

2019 ◽

Vol 294 (15) ◽

pp. 6073-6081 ◽

Cited By ~ 6

Author(s):

Yan Su ◽

Pratibha P. Ghodke ◽

Martin Egli ◽

Lin Li ◽

Yinsheng Wang ◽

...

Keyword(s):

Reverse Transcriptase ◽

Dna Polymerase ◽

Reverse Transcriptase Activity ◽

Transcriptase Activity ◽

Human Dna

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