Chaga mushroom extract inhibits oxidative DNA damage in human lymphocytes as assessed by comet assay

BioFactors ◽  
2004 ◽  
Vol 21 (1-4) ◽  
pp. 109-112 ◽  
Author(s):  
Yoo Kyoung Park ◽  
Hyang Burm Lee ◽  
Eun-Jae Jeon ◽  
Hack Sung Jung ◽  
Myung-Hee Kang
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Oxidative Dna Damage ◽  
Human Lymphocytes ◽  
Mushroom Extract

Effect of Ascorbic Acid (Vitamin C) on H2O2 Induced Oxidative DNA Damage in Human Lymphocytes Estimated by Comet Assay

2018 ◽  
Vol 14 (1) ◽  
pp. 204-217
Author(s):  
Hanaa Naji Abdullah ◽  
◽  
Sura Ali Al Asadi ◽  
Mohammed Abduldaim Saleh
Keyword(s):  
Dna Damage ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Comet Assay ◽  
Oxidative Dna Damage ◽  
Human Lymphocytes

Protective effects of aqueous and ethanol extracts of rosemary on H2O2-induced oxidative DNA damage in human lymphocytes by comet assay

2014 ◽  
Vol 11 (1) ◽  
Author(s):  
Kamal Razavi-Azarkhiavi ◽  
Javad Behravan ◽  
Fatemeh Mosaffa ◽  
Samira Sehatbakhsh ◽  
Kobra Shirani ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Oxidative Dna Damage ◽  
Human Lymphocytes ◽  
Protective Effects ◽  
Ethanol Extracts

Basal damage and oxidative DNA damage in children with chronic kidney disease measured by use of the comet assay

2011 ◽  
Vol 725 (1-2) ◽  
pp. 22-28 ◽  
Author(s):  
Banu Aykanat ◽  
Gonca Cakmak Demircigil ◽  
Kibriya Fidan ◽  
Necla Buyan ◽  
Kaan Gulleroglu ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Dna Damage ◽  
Kidney Disease ◽  
Comet Assay ◽  
Oxidative Dna Damage

DNA damage assessment by comet assay of human lymphocytes exposed to jet propulsion fuels

2002 ◽  
Vol 40 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Shawna M. Jackman ◽  
Geraldine M. Grant ◽  
Christopher J. Kolanko ◽  
David A. Stenger ◽  
Joginder Nath
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Damage Assessment ◽  
Human Lymphocytes ◽  
Jet Propulsion

Effect of phytoestrogen and antioxidant supplementation on oxidative DNA damage assessed using the comet assay

2001 ◽  
Vol 485 (2) ◽  
pp. 169-176 ◽  
Author(s):  
Jayne Sierens ◽  
John A. Hartley ◽  
Maeli J. Campbell ◽  
Anthony J.C. Leathem ◽  
Jayne V. Woodside
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Oxidative Dna Damage ◽  
Antioxidant Supplementation

Induction of oxidative DNA damage and enhancement of cell proliferation in human lymphocytes in vitro by butylated hydroxyanisole

1995 ◽  
Vol 16 (3) ◽  
pp. 507-512 ◽  
Author(s):  
P.A.E.L. Schilderman ◽  
E. Rhijnsburger ◽  
I. Zwingmann ◽  
J.C.S. Kleinjans
Keyword(s):  
Cell Proliferation ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Human Lymphocytes ◽  
Butylated Hydroxyanisole

In vivo supplementation with coenzyme Q 10 enhances the recovery of human lymphocytes from oxidative DNA damage

2001 ◽  
Vol 15 (8) ◽  
pp. 1425-1427 ◽  
Author(s):  
Marco Tomasetti ◽  
Renata Alleva ◽  
Andrew R. Collins
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Coenzyme Q ◽  
Human Lymphocytes

Imatinib (STI571) Induces DNA Damage in BCR/ABL-Expressing Leukemic Cells but Not in Normal Lymphocytes.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4353-4353
Author(s):  
Janusz Blasiak ◽  
Jozef Drzewoski ◽  
Tomasz Poplawski ◽  
Agnieszka Czechowska
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Lymphocytes ◽  
Direct Interaction ◽  
K562 Cells ◽  
Dna Lesions ◽  
Dna Glycosylase ◽  
Leukemic Cells ◽  
Drug Induced ◽  
Strand Breaks

Abstract Imatinib (STI571) is a 2-phenylaminopyrimidine derivative used mostly in the treatment of chronic myeloid leukaemia. It targets specifically the BCR/ABL oncogenic tyrosine kinase, inhibiting its activity. Using the alkaline comet assay we showed that STI571 at concentrations ranging from 0.05 to 2 μM induced DNA damage in human leukemic K562 cells expressing the BCR/ABL oncogene, whereas it had no effect in normal human lymphocytes. Because the extent of DNA damage observed in the neutral and pH 12.1 versions of the comet assay was much lesser than in the alkaline version, we concluded that the drug induced DNA alkali-labile sites rather than strand breaks. Imatinib did not induce DNA strand breaks in the direct interaction with DNA as examined by the plasmid relaxation assay. K562 cells were unable to repair H2O2-induced DNA damage during a 120-min incubation, if they had been preincubated with STI571, whereas normal lymphocytes did so within 60 min. Pre-treatment of K562 cells with vitamins A, C and E reduced the extent of DNA damage evoked by STI571. Similar results brought experiments with the nitrone spin traps POBN and PBN, suggesting that free radicals may be involved in the formation of DNA lesions induced by STI571 in K562 cells. These cells exposed to imatinib and treated with endonuclease III, formamidopyrimidine-DNA glycosylase and 3-methyladenine-DNA glycosylase II, the enzymes recognizing oxidized and alkylated bases, displayed greater extent of DNA damage than those not treated with these enzymes.

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