Amniotic fluid stem cell exosomes: Therapeutic perspective

BioFactors ◽  
2018 ◽  
Vol 44 (2) ◽  
pp. 158-167 ◽  
Author(s):  
Francesca Beretti ◽  
Manuela Zavatti ◽  
Francesca Casciaro ◽  
Giuseppina Comitini ◽  
Fabrizia Franchi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Amniotic Fluid Stem Cell ◽  
Therapeutic Perspective

scholarly journals Autophagy is impaired in fetal hypoplastic lungs and rescued by administration of amniotic fluid stem cell extracellular vesicles

2022 ◽  
Author(s):  
Kasra Khalaj ◽  
Lina Antounians ◽  
Rebeca Lopes Figueira ◽  
Martin Post ◽  
Augusto Zani
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Extracellular Vesicles ◽  
Pulmonary Hypoplasia ◽  
Fetal Lung ◽  
Branching Morphogenesis ◽  
Lung Epithelial Cells ◽  
Normal Lung ◽  
Amniotic Fluid Stem Cell ◽  
Human Fetal Lung

Rationale: Pulmonary hypoplasia secondary to congenital diaphragmatic hernia (CDH) is characterized by reduced branching morphogenesis, which is responsible for poor clinical outcomes. Administration of amniotic fluid stem cell extracellular vesicles (AFSC-EVs) rescues branching morphogenesis in rodent fetal models of pulmonary hypoplasia. Herein, we hypothesized that AFSC-EVs exert their regenerative potential by affecting autophagy, a process required for normal lung development. Objectives: To evaluate autophagy in hypoplastic lungs throughout gestation and establish whether AFSC-EV administration improves branching morphogenesis through autophagy-mediated mechanisms. Methods: EVs were isolated from c-kit+ AFSC conditioned medium by ultracentrifugation and characterized for size, morphology, and EV markers. Branching morphogenesis was inhibited in rat fetuses by nitrofen administration to dams and in human fetal lung explants by blocking RAC1 activity with NSC23766. Expression of autophagy activators (BECN1 and ATG5) and adaptor (SQSTM1/p62) was analyzed in vitro (rat and human fetal lung explants) and in vivo (rat fetal lungs). Mechanistic studies on rat fetal primary lung epithelial cells were conducted using inhibitors for microRNA-17 and -20a contained in the AFSC-EV cargo and known to regulate autophagy. Measurements and Main Results: Rat and human models of fetal pulmonary hypoplasia showed reduced autophagy mainly at pseudoglandular and canalicular stages. AFSC-EV administration restored autophagy in both pulmonary hypoplasia models by transferring miR-17~92 cluster members contained in the EV cargo. Conclusions: AFSC-EV treatment rescues branching morphogenesis partly by restoring autophagy through miRNA cargo transfer. This study enhances our understanding of pulmonary hypoplasia pathogenesis and creates new opportunities for fetal therapeutic intervention in CDH babies.

scholarly journals Calcification after myocardial infarction is independent of amniotic fluid stem cell injection

2011 ◽  
Vol 20 (2) ◽  
pp. e69-e78 ◽  
Author(s):  
Dawn M. Delo ◽  
Xuan Guan ◽  
Zhan Wang ◽  
Leanne Groban ◽  
Michael Callahan ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Stem Cell ◽  
Amniotic Fluid ◽  
Cell Injection ◽  
Amniotic Fluid Stem Cell ◽  
Stem Cell Injection

The Amniotic Fluid Stem Cell Secretome

2018 ◽  
pp. 21-37
Author(s):  
Sveva Bollini ◽  
Carolina Balbi ◽  
Martina Piccoli ◽  
Michela Pozzobon
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Amniotic Fluid Stem Cell

scholarly journals The Regenerative Potential of Amniotic Fluid Stem Cell Extracellular Vesicles: Lessons Learned by Comparing Different Isolation Techniques

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Lina Antounians ◽  
Areti Tzanetakis ◽  
Ornella Pellerito ◽  
Vincenzo D. Catania ◽  
Adrienne Sulistyo ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Extracellular Vesicles ◽  
Lessons Learned ◽  
Isolation Techniques ◽  
Amniotic Fluid Stem Cell

Critical-size bone defect repair using amniotic fluid stem cell/collagen constructs: Effect of oral ferutinin treatment in rats

Life Sciences ◽  
2015 ◽  
Vol 121 ◽  
pp. 174-183 ◽  
Author(s):  
Manuela Zavatti ◽  
Laura Bertoni ◽  
Tullia Maraldi ◽  
Elisa Resca ◽  
Francesca Beretti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Bone Defect ◽  
Critical Size ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Amniotic Fluid Stem Cell

scholarly journals First steps to define murine amniotic fluid stem cell microenvironment

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
E. Bertin ◽  
M. Piccoli ◽  
C. Franzin ◽  
G. Spiro ◽  
S. Donà ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Cell Microenvironment ◽  
Amniotic Fluid Stem Cell

The human amniotic fluid stem cell secretome exerts cardio-active paracrine potential for myocardial repair and regeneration

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S171
Author(s):  
A. Costa ◽  
C. Balbi ◽  
P.R. Garbati ◽  
K. Lodder ◽  
T. van Herwaarden ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Myocardial Repair ◽  
Human Amniotic Fluid ◽  
Amniotic Fluid Stem Cell

Amniotic fluid stem cell administration can prevent epithelial injury from necrotizing enterocolitis

2021 ◽  
Author(s):  
Bo Li ◽  
Carol Lee ◽  
Marissa Cadete ◽  
Joshua S. O’Connell ◽  
Mashriq Alganabi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Amniotic Fluid ◽  
Necrotizing Enterocolitis ◽  
Epithelial Injury ◽  
Amniotic Fluid Stem Cell

scholarly journals Bladder Transplantation of Amniotic Fluid Stem Cell may Ameliorate Bladder Dysfunction After Focal Cerebral Ischemia in Rat

2017 ◽  
Vol 6 (4) ◽  
pp. 1227-1236 ◽  
Author(s):  
Ching-Chung Liang ◽  
S.W. Steven Shaw ◽  
Yung-Hsin Huang ◽  
Yi-Hao Lin ◽  
Tsong-Hai Lee
Keyword(s):  
Stem Cell ◽  
Cerebral Ischemia ◽  
Amniotic Fluid ◽  
Bladder Dysfunction ◽  
Focal Cerebral Ischemia ◽  
Amniotic Fluid Stem Cell
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