Flow injection chemiluminescent assays for glycerol and triglycerides using a co-immobilized enzyme reactor

Luminescence ◽  
2003 ◽  
Vol 18 (2) ◽  
pp. 67-71 ◽  
Author(s):  
M. Yaqoob ◽  
A. Nabi
Keyword(s):  
Flow Injection ◽  
Immobilized Enzyme ◽  
Enzyme Reactor ◽  
Immobilized Enzyme Reactor

scholarly journals Fluorometric Determination of Ethanol in Liquor Samples by Flow-Injection Analysis Using an Immobilized Enzyme-Reactor Column with Packing Prepared by Coupling Alcohol Oxidase and Peroxidase onto Chitosan Beads

2001 ◽  
Vol 84 (5) ◽  
pp. 1475-1484 ◽  
Author(s):  
Tetsuyuki Taniai ◽  
Akio Sukuragawa ◽  
Tadao Okutani
Keyword(s):  
Flow Injection ◽  
Immobilized Enzyme ◽  
Alcohol Oxidase ◽  
Injection System ◽  
Determination Limit ◽  
Enzyme Reactor ◽  
Chitosan Beads ◽  
Immobilized Enzyme Reactor ◽  
Analytical Response

Abstract A flow-injection system was developed for the determination of ethanol with an immobilized enzyme-reactor column. This system, which consisted of hand-made reactor columns packed with alcohol oxidase and horseradish peroxidase immobilized onto chitosan beads, and a fluorometric detector, was applied to the determination of ethanol in liquor samples. Under the recommended conditions, the ethanol, which was present in the pretreated samples, was converted to hydrogen peroxide when it was passed through the immobilized alcohol oxidase (AOD) column with 0.1 mol/dm3 phosphate buffer (pH 7.0). A sample can be analyzed with this system in <10 min. The calibration curve for ethanol was linear from 2.0 to 0.1 mg/dm3. The determination limit, which was defined by the difference between the sample peak and blank peak, was estimated to be 50 μg/dm3 for ethanol. Interferences from some substances present in actual liquor samples decreased the analytical response and activity of the immobilized AOD-reactor column, but they were removed by dilution and pretreatment with an octyldecylsilane cartridge.

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