Resonance Rayleigh scattering and spectrofluorimetric approaches for the selective determination of rupatadine using erythrosin B as a probe: application to content uniformity test

Luminescence ◽  
2020 ◽  
Author(s):  
Albandary Almahri ◽  
Mohamed A. Abdel‐Lateef ◽  
Ebtihal Samir ◽  
Sayed M. Derayea ◽  
Mohamed A. El Hamd
Keyword(s):  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Content Uniformity ◽  
Erythrosin B ◽  
Selective Determination ◽  
Uniformity Test

scholarly journals Rapid and Selective Determination of Folate Receptor α with Sensitive Resonance Rayleigh Scattering Signal

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Liping Wu ◽  
Yue Liu ◽  
Rong Huang ◽  
Huawen Zhao ◽  
Weiqun Shu
Keyword(s):  
Rayleigh Scattering ◽  
Folate Receptor ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Au Nps ◽  
Selective Determination ◽  
Limit Of Determination

A rapid, simple, and novel method for folate receptor α (FRα) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FRα antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FRα was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity (ΔIRRS) and the concentration of FRα, with the detecting range of 0.50–37.50 ng·mL−1 and the limit of determination of 0.05 ng·mL−1. The determination of FRα in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation.

scholarly journals Xanthene based resonance Rayleigh scattering and spectrofluorimetric probes for the determination of cyclobenzaprine: Application to content uniformity test

2020 ◽  
Vol 39 (1) ◽  
pp. 222-230 ◽  
Author(s):  
Mohamed A. Abdel-Lateef ◽  
Albandary Almahri ◽  
Sayed M. Derayea ◽  
Ebtihal Samir
Keyword(s):  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Excitation Wavelength ◽  
Content Uniformity ◽  
Scattering Method ◽  
Eosin Y ◽  
Limit Values ◽  
Quenching Effect ◽  
Scattering Spectrum ◽  
Uniformity Test

Abstract In this work, a resonance Rayleigh scattering technique and a spectrofluorimetric technique were applied to the quantification of cyclobenzaprine through two validated methods. The suggested methods are based on a facile association complex formation between cyclobenzaprine and eosin Y reagent in the acidic medium. The resonance Rayleigh scattering method relied on the enhancement in the resonance Rayleigh scattering spectrum of eosin Y at 370 nm after the addition of cyclobenzaprine. On the other hand, the spectrofluorimetric quantification relied on the quenching effect of cyclobenzaprine on the fluorescence strength of the eosin Y reagent at 545 nm (excitation wavelength at 300 nm). The suggested methods were linear over the ranges of 0.07–1.75 μg/mL and 0.15–2.0 μg/mL with detection limit values of 0.023 μg/mL and 0.048 μg/mL for the resonance Rayleigh scattering method and the spectrofluorimetric method, respectively. All reaction conditions for cyclobenzaprine–eosin Y formation were experimentally evaluated and optimized. In addition, both methods were validated based on ICH rules. Furthermore, the developed methods were practically applied to the analysis of cyclobenzaprine in its commercial tablet dosage form with acceptable recoveries. Moreover, the content uniformity test of the commercial cyclobenzaprine tablets was successfully applied using the proposed spectroscopic methods based on USP rules.

Resonance Rayleigh Scattering Method for the Determination of Raloxifene with Evans Blue

2010 ◽  
Vol 20 (12) ◽  
pp. 1552-1556 ◽  
Author(s):  
Li Fan ◽  
Shao-Pu Liu ◽  
Da-Cheng Yang ◽  
Xiao-Li Hu
Keyword(s):  
Evans Blue ◽  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Scattering Method

Resonance Rayleigh Scattering Spectral Determination of As(V) Using Cu(II) as Catalyst

2013 ◽  
Vol 788 ◽  
pp. 23-26
Author(s):  
Gui Qing Wen ◽  
Ai Hui Liang
Keyword(s):  
Waste Water ◽  
Detection Limit ◽  
Spectral Method ◽  
Correlation Coefficient ◽  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Spectral Determination ◽  
Strong Resonance ◽  
Hcl Medium

In HCl medium and in the presence of CuSO4, Na3AsO4 can be reduced by NaH2PO2 to form As nanoparticles (AsNs) which exhibited a strong resonance Rayleigh scattering (RRS) peak at 370 nm. Under the chosen conditions, the increased intensity at 370 nm was linear to As5+ concentration in the range of 0.48-38.0×10-6 mol/L, with a regression equation of ΔI370nm = 82.3 CAs + 33.9, a correlation coefficient of 0.9878 and a detection limit of 2.0×10-7 mol/L As5+. The proposed method was applied to detect As5+ concentration in waste water, with simplicity, rapidity and accuracy. Thus, a novel RRS spectral method was established to determine As5+.

Resonance Rayleigh Scattering Determination of Trace Hemin Basing on Aptamer-Modified Nanogold Catalysis of HAuCl4-Vitamine C

2013 ◽  
Vol 787 ◽  
pp. 400-403
Author(s):  
Jin Chao Dong ◽  
Ai Hui Liang ◽  
Zhi Liang Jiang
Keyword(s):  
Detection Limit ◽  
Rayleigh Scattering ◽  
Resonance Rayleigh Scattering ◽  
Serum Samples ◽  
Buffer Solution ◽  
Strong Resonance ◽  
Scattering Spectra ◽  
Vitamine C ◽  
Nanogold Catalysis

Hemin aptamer was used to modify gold nanoparticles (AuNPs) to obtain a stable aptamer-nanogold probe (AussDNA). In the condition of pH 8.0 Tris-HCl buffer solution containing 50mmol/L NaCl, the substrate chain of AussDNA was cracked by hemin to produce a short single-stranded DNA(ssDNA) and then further combined with hemin to form a stable hemin-ssDNA conjugate. The AuNPs released from AussDNA would be aggregated in the condition of 50mmol/L NaCl and exhibited a strong resonance Rayleigh scattering (RRS) peak at 368nm. Under the selected conditions, the increased RRS intensity (ΔI368nm) was linear to hemin concentration in the range of 5-750nmol/L, with a detection limit of 66 pmol/L. This RRS method was applied to determination of residual hemin in serum samples, with satisfactory results. The remnant AussDNA in the solution exhibited a strong catalytic activity on the gold particle reaction of HAuCl4-vitamine C (VC) that can be monitored by RRS technique at 368 nm. When the hemin concentration increased, the AussDNA decreased, the catalysis decreased, and the RRS intensity at 368nm decreased. The decreased RRS intensity ΔI368nmwas linear to the hemin concentration in the range of 1-200nmol/L, with a detection limit of 54 pmol/L. Accordingly, a sensitivity, selectivity, and simplicity new method of resonance Rayleigh scattering spectra to detect hemin using aptamer-modified nanogold as catalyst was established.

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