A simple and rapid spectrofluorometric determination of pomalidomide in spiked plasma and urine. Application to degradation studies

Luminescence ◽  
2019 ◽  
Vol 35 (4) ◽  
pp. 466-477 ◽  
Author(s):  
Z. Aydoğmuş ◽  
E.M. Yılmaz ◽  
G. Yildiz
Keyword(s):  
Spectrofluorometric Determination ◽  
Spiked Plasma ◽  
Degradation Studies

scholarly journals Spectrofluorometric Determination of Fluvoxamine in Dosage Forms, Spiked Plasma, and Real Human Plasma by Derivatization with Fluorescamine

2007 ◽  
Vol 90 (2) ◽  
pp. 376-383 ◽  
Author(s):  
Nahed El-Enany
Keyword(s):  
Human Plasma ◽  
Reaction Pathway ◽  
Biological Fluids ◽  
Pharmaceutical Formulations ◽  
Dosage Forms ◽  
Spectrofluorometric Determination ◽  
Spiked Plasma ◽  
Experimental Parameters ◽  
Good Agreement

Abstract A sensitive, simple, and selective spectrofluorometric method was developed for the determination of fluvoxamine (FXM) in pharmaceutical formulations and biological fluids. The method is based upon the reaction between the drug and fluorescamine in borate buffer of pH 8.0 to yield a highly fluorescent derivative that is measured at 481 nm after excitation at 383 nm. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. The method was applied for the determination of the drug over the concentration range of 0.11.1 μg/mL with a detection limit of 0.01 μg/mL (2 10-8 M). The proposed method was successfully applied to the analysis of commercial tablets. The results obtained were in good agreement with those obtained using a reported spectrophotometric method. The method was applied for the determination of FXM in spiked human plasma with recovery (n = 4) of 97.32 1.23%, while that in real human plasma (n = 3) was 90.79 2.73%. A proposal for the reaction pathway is presented.

Spectrophotometric and Spectrofluorometric Determination of Heptaminol and mexiletine in Their Dosage Forms

1997 ◽  
Vol 30 (11) ◽  
pp. 2029-2043 ◽  
Author(s):  
Abdel Fattah M. El Walily ◽  
Fawzy A. El-Yazbi ◽  
Saeid F. Belal ◽  
Omayma Abdel-Razak
Keyword(s):  
Dosage Forms ◽  
Spectrofluorometric Determination

RP-HPLC Method for Determination of Oseltamivir Phosphate in Capsules and Spiked Plasma

2010 ◽  
Vol 43 (14) ◽  
pp. 2200-2209 ◽  
Author(s):  
Zeynep Aydoğmuş ◽  
Sena Çağlar ◽  
Sıdıka Toker
Keyword(s):  
Hplc Method ◽  
Rp Hplc ◽  
Spiked Plasma ◽  
Oseltamivir Phosphate

Use of 1-Hydroxy-2-Carboxyanthraquinone for the Spectrofluorometric Determination of Be(II)

1975 ◽  
Vol 8 (10) ◽  
pp. 753-761 ◽  
Author(s):  
F. Capitan ◽  
F. Salinas ◽  
L. M. Franquelo
Keyword(s):  
Spectrofluorometric Determination

A new selective reagent for the spectrofluorometric determination of beryllium

1976 ◽  
Vol 21 (4) ◽  
pp. 402-410 ◽  
Author(s):  
V. Drevenkar ◽  
Z. Štefanac ◽  
A. Brbot
Keyword(s):  
Spectrofluorometric Determination ◽  
Selective Reagent

scholarly journals Spectrofluorometric determination of L-tryptophan in canary (Canarium indicum L.) seed protein hydrolysate

2019 ◽  
Vol 10 (1) ◽  
pp. 4780-4785
Keyword(s):  
Protein Hydrolysate ◽  
Intermediate Precision ◽  
North Sulawesi ◽  
Accuracy Test ◽  
Spectrofluorometric Determination ◽  
Relative Standard ◽  
Indigenous Plant ◽  
Assay Precision ◽  
Specific Reagent

Canary (Canarium indicum L.) is an indigenous plant of Indonesia, which mainly grows in the eastern part of Indonesia, especially in the Maluku, North Sulawesi, and Seram islands. We believe that no scientific reports have been conducted about L-tryptophan content in Canarium indicum. Therefore, this study was conducted to determine the presence and quantitate the aromatic amino acid (L-tryptophan) in the canary protein hydrolysate by the spectrofluorometric method. The protein hydrolysate was prepared by two hydrolysis methods, enzymatic and alkaline hydrolysis. L-tryptophan can be differentiated from tyrosine directly without using any reagent by excitation of the sample at 295 nm in order to avoid tyrosine emission. The equation of calibration curve correlation using standard in the range 0.5-5 ppm was y = 6632.3x - 845.42 and correlation coefficient of 0.9997, while the coefficient of variance in linear regression was 1.29%. The detection limit and quantification limit obtained were 0.116 ppm and 0.35 ppm respectively. The recoveries of the accuracy test were obtained in the range of 95-96%. The relative standard deviation of intra-assay precision tests were obtained in the range of 0.5-1.8%, while the intermediate precision in the range of 2.18-3.74%. L-tryptophan was detected in all samples (papain, pepsin, and alkaline hydrolysate), with concentrations 5.6, 5 and 1.53 mg/100mg of protein respectively. The used fluorometric method complied with the validation requirements and can be used to analyze L-tryptophan in samples containing tyrosine without overlapping of spectra and without the use of any specific reagent.

scholarly journals Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

2006 ◽  
Vol 22 (2) ◽  
pp. 263-267 ◽  
Author(s):  
Hamid ABDOLLAHI ◽  
Mohammad Hossein SORORADDIN ◽  
Abdolhossein NASERI
Keyword(s):  
Spectrofluorometric Determination

Supramolecular complexation between cucurbit[7]uril and folate and analytical applications

2021 ◽  
pp. 174751982110664
Author(s):  
Jue Chen ◽  
Tengmei Gao ◽  
Yinxia Chang ◽  
Yanming Wei ◽  
Yonghui Wang
Keyword(s):  
Fluorescence Spectra ◽  
Fluorescence Probe ◽  
H Nmr ◽  
Fluorescent Method ◽  
Inclusion Interaction ◽  
Spiked Plasma ◽  
Direct Fluorescence ◽  
Supramolecular Complexation

Folate (FA) plays a key role in the biosynthesis of amino acids, purines, and pyrimidines in the human body, and intracellular folate metabolism has become an attractive target of tumor chemotherapy. In this work, an inclusion interaction was found between FA and cucurbit[7]uril (CB[7]), and the formation of a CB[7]-FA 2:1 supramolecular inclusion complex was confirmed by fluorescence spectra, UV-Vis absorption spectroscopy, 1H NMR, and molecular modeling calculations. In addition, FA is generally determined through the indirect fluorescent method because it shows weak fluorescence in aqueous solution. Therefore, a simple, direct fluorescence probe method for rapidly measuring FA was investigated, and the linear equation of FA was ΔF = 14.691C + 37.366 within the concentration ranges of 0.82 ~ 18.31 µg mL–1. The proposed direct fluorescence method was applied to the determination of spiked plasma. We demonstrated that this method could provide an experimental basis for the targeted administration of the CB[7]-FA complex, and it could be extended as a promising fluorescence detection method for drugs in vivo.

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