Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1‐blocker drugs: Application in pharmaceutical preparations and human plasma

Luminescence ◽  
2018 ◽  
Vol 33 (7) ◽  
pp. 1226-1234 ◽  
Author(s):  
Mahmoud A. Omar ◽  
Mohamed A. Hammad ◽  
Baher I. Salman
Keyword(s):  
Human Plasma ◽  
Pharmaceutical Preparations ◽  
Α1 Blocker

scholarly journals Spectrofluorometric Determination of Verapamil Hydrochloride in Pharmaceutical Preparations and Human Plasma Using Organized Media: Application to Stability Studies

2006 ◽  
Vol 89 (6) ◽  
pp. 1565-1572 ◽  
Author(s):  
Mohamed Walash ◽  
Fathalla Belal ◽  
Nahed El-Enany ◽  
Amina Abdelsalam
Keyword(s):  
Human Plasma ◽  
Biological Fluids ◽  
Sodium Dodecyl ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Official Method ◽  
Stability Studies ◽  
Verapamil Hydrochloride ◽  
Spiked Human Plasma

Abstract A highly sensitive spectrofluorometric method was developed for the determination of verapamil hydrochloride (VP HCl) in pharmaceutical formulations and biological fluids. The proposed method is based on investigation of the fluorescence spectral behavior of VP HCl in micellar systems, such as sodium dodecyl sulfate (SDS) and β-cyclodextrin (β-CD). In aqueous solutions of borate buffer of pH 9 and 8.5, VP HCl was well incorporated into SDS and β-CD, respectively, with enhancement of its native fluorescence. The fluorescence was measured at 318 nm after excitation at 231 nm. The fluorescence intensity enhancements were 183 and 107% in SDS and in β-CD, respectively. The fluorescence-concentration plots were rectilinear over the range of 0.020.2 and 0.020.25 μg/mL, with lower detection limits of 5.58 × 103 and 3.62 × 103 μg/mL in SDS and β-CD, respectively. The method was successfully applied to the analysis of commercial tablets and the results were in good agreement with those obtained with the official method. The method was further applied to the determination of VP HCl in real and spiked human plasma. The mean % recoveries in the case of spiked human plasma (n 4) was 92.59 3.11 and 88.35 2.55 using SDS and β-CD, respectively, while that in real human plasma (n 3) was 90.17 6.93 and 89.17 6.50 using SDS and β-CD, respectively. The application of the method was extended to the stability studies of VP HCl after exposureto ultraviolet radiation and upon oxidation with hydrogen peroxide.

scholarly journals Simultaneous Determination of Sitagliptin and Metformin in Pharmaceutical Preparations by Capillary Zone Electrophoresis and its Application to Human Plasma Analysis

2012 ◽  
Vol 7 ◽  
pp. ACI.S9940 ◽  
Author(s):  
Mohamed Salim ◽  
Nahed El-Enany ◽  
Fathallah Belal ◽  
Mohamed Walash ◽  
Gabor Patonay
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Capillary Zone Electrophoresis ◽  
Pharmaceutical Preparations ◽  
Effective Length ◽  
Relative Standard ◽  
Significant Difference ◽  
Zone Electrophoresis ◽  
Capillary Zone

A novel, quick, reliable and simple capillary zone electrophoresis CZE method was developed and validated for the simultaneous determination of sitagliptin (SG) and metformin (MF) in pharmaceutical preparations. Separation was carried out in fused silica capillary (50.0 cm total length and 43.0 cm effective length, 49 μm i.d.) by applying a potential of 15 KV (positive polarity) and a running buffer containing 60 mM phosphate buffer at pH 4.0 with UV detection at 203 nm. The samples were injected hydrodynamically for 3 s at 0.5 psi and the temperature of the capillary cartridge was kept at 25 °C. Phenformin was used as internal standard (IS). The method was suitably validated with respect to specificity, linearity, limit of detection and quantitation, accuracy, precision, and robustness. The method showed good linearity in the ranges of 10-100 μg/mL and 50-500 μg/mL with limits of detection of 0.49, 2.11 μm/mL and limits of quantification of 1.48, 6.39 μg/mL for SG and MF, respectively. The proposed method was successfully applied for the analysis of the studied drugs in their synthetic mixtures and co-formulated tablets without interfering peaks due to the excipients present in the pharmaceutical tablets. The method was further extended to the in-vitro determination of the two drugs in spiked human plasma. The estimated amounts of SG/MF were almost identical with the certified values, and their percentage relative standard deviation values (% R.S.D.) were found to be ≤1.50% (n = 3). The results were compared to a reference method reported in the literature and no significant difference was found statistically.

scholarly journals Simultaneous analysis of aliskiren and hydrochlorothiazide in pharmaceutical preparations and spiked human plasma by HPTLC

2017 ◽  
Vol 11 (5) ◽  
pp. 667-676 ◽  
Author(s):  
Jui J. Pandya ◽  
Nejal M. Bhatt ◽  
Vijay D. Chavada ◽  
Primal Sharma ◽  
Mallika Sanyal ◽  
...  
Keyword(s):  
Human Plasma ◽  
Pharmaceutical Preparations ◽  
Simultaneous Analysis ◽  
Spiked Human Plasma

Poly (vinyl chloride) matrix membrane sensors for the quantification of olopatadine and oxeladine in pharmaceutical preparations and human plasma

2019 ◽  
Vol 147 ◽  
pp. 170-175
Author(s):  
Mahmoud M. Sebaiy ◽  
Mohamed A.F. Elmosallamy ◽  
Magda M. Elhenawee ◽  
Mustafa Kh. Alshuwaili
Keyword(s):  
Human Plasma ◽  
Vinyl Chloride ◽  
Pharmaceutical Preparations ◽  
Poly Vinyl Chloride ◽  
Chloride Matrix

Micellar-Enhanced Spectrofluorimetric Method for Quantification of Diclofenac Potassium in Pure Form, in Pharmaceutical Preparations and Human Plasma

2021 ◽  
Vol 58 (6) ◽  
pp. 427-434
Author(s):  
Muhammad Naeem Khan ◽  
Irum ◽  
Saba Gul ◽  
Muslima ◽  
Muhammad Mursaleen
Keyword(s):  
Human Plasma ◽  
Limit Of Detection ◽  
Sodium Dodecyl ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Products ◽  
Pure Form ◽  
Fluorescence Signal ◽  
Spectrofluorimetric Method ◽  
Relative Standard

Abstract A rapid, simple and economical spectrofluorimetric method for the determination of diclofenac potassium in pure form, in pharmaceutical preparations and in human plasma has been developed. The method is based on the enhancement of the fluorescence signal of diclofenac potassium by the addition of sodium dodecyl sulphate in McIvaine buffer with a pH of 5. Different experimental conditions such as buffer type, pH, type and concentration of surfactants were investigated. The fluorescence intensity of the solution was recorded at 361 nm after excitation at 243 nm. The method shows linearity in the concentration range of 0.2 μg mL–1–10 μg mL–1 with a good correlation coefficient of 0.997. The relative standard deviation value was 3.62 (n = 7). The limit of detection and limit of quantification were calculated to be 2.84 × 10–3 μg mL–1 and 9.47 × 10–3 μg mL-1, respectively. The effect of excipients and co-administrated drugs was investigated and no interference was observed. The method was successfully applied for the determination of diclofenac potassium in pure form, in pharmaceutical products and in human plasma. The percentage recoveries obtained ranged from 100.25% to 102.16% for pure form and 97.50% to 102.00% for pharmaceutical products and from 98.50% to 101.67% for human plasma.

scholarly journals Spectrofluorometric Determination of Citalopram in Pharmaceutical Preparations and Spiked Human Plasma Using Organized Media

2006 ◽  
Vol 89 (5) ◽  
pp. 1288-1295 ◽  
Author(s):  
Dina T El-Sherbiny
Keyword(s):  
Aqueous Solution ◽  
Human Plasma ◽  
Fluorescence Intensity ◽  
Pharmaceutical Preparations ◽  
Citrate Buffer ◽  
Acidic Aqueous Solution ◽  
Sds Micelles ◽  
Spectrofluorometric Determination ◽  
Organized Media

Abstract The native fluorescence of citalopram (CIT) was (obtained in citrate buffer of pH 6.5 with and without β-cyclodextrin (β-CD) or sodium dodecyl sulfate (SDS) as fluorescence enhancers at 305 nm using 242 nm for excitation. Micellar systems of ionic and nonionic surfactants were investigated by measuring the fluorescence intensity of the analyte-surfactant system. In slightly acidic aqueous solution of pH 6.5, CIT was better incorporated in CDs and SDS micelles. The luminescence emission from CIT was found to be greatly enhanced by SDS micelles. The fluorescence intensity enhancements in CDs medium and in SDS as ionic surfactant relative to slightly acidic aqueous solution were 125 and 250%, respectively. Organized media-enhanced spectroflourometric methods were developed for the determination of CIT, in pure form as well as in pharmaceutical preparations. The fluorescence intensity-concentration plots were rectilinear over the ranges 0.06 to 0.64, 0.04 to 0.40, and 0.02 to 0.26 μg/mL with lower detection limits of 0.02, 0.01, and 0.007 μg/mL, either in citrate buffer only or in β-CD and SDS as organized media, respectively. Furthermore, the high sensitivity attained by using SDS as organized medium allowed in vitro spectrofluorometric determination of CIT in spiked human plasma. Interference from endogenous amino acids has been overcome by using the solid-phase extraction technique; the mean recovery (n = 5) was 100.1 ± 0.8%

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