Chemiluminescence analysis of antioxidant capacity for serum albumin isolated from healthy or uremic volunteers

Luminescence ◽  
2016 ◽  
Vol 31 (8) ◽  
pp. 1474-1478 ◽  
Author(s):  
Chih-Yang Huang ◽  
Show-Yih Liou ◽  
Wei-Wen Kuo ◽  
Hsi-Chin Wu ◽  
Yen-Lin Chang ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Antioxidant Capacity ◽  
Chemiluminescence Analysis

scholarly journals Polyphenol capping on a gold nanosurface modulates human serum albumin fibrillation

2020 ◽  
Vol 1 (5) ◽  
pp. 1142-1150
Author(s):  
Aalok Basu ◽  
Sonia Kundu ◽  
Aatrayee Das ◽  
Chitra Basu ◽  
Sagar Bhayye ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Antioxidant Capacity ◽  
Human Serum

The antioxidant capacity inherent to polyphenol-capped gold nanoparticles contributed to the inhibition of human serum albumin fibrillation.

Antioxidant capacity and structural changes of human serum albumin from patients in advanced stages of diabetic nephropathy and the effect of the dialysis

2015 ◽  
Vol 404 (1-2) ◽  
pp. 193-201 ◽  
Author(s):  
Marisol Rosas-Díaz ◽  
Menandro Camarillo-Cadena ◽  
Andrés Hernández-Arana ◽  
Eva Ramón-Gallegos ◽  
Rafael Medina-Navarro
Keyword(s):  
Diabetic Nephropathy ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Antioxidant Capacity ◽  
Human Serum ◽  
Structural Changes ◽  
Advanced Stages

scholarly journals Inhibition of Advanced Glycation End-Product Formation by High Antioxidant-Leveled Spices Commonly Used in European Cuisine

Antioxidants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 100 ◽  
Author(s):  
Małgorzata Starowicz ◽  
Henryk Zieliński
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Antioxidant Capacity ◽  
Bovine Serum ◽  
Radical Scavenging ◽  
Model Systems ◽  
Total Phenolic ◽  
Advanced Glycation ◽  
Advanced Glycation End Product ◽  
Star Anise

Spices and herbs, as good sources of polyphenols, could be strong inhibitors of advanced glycation end-product (AGE) formation. The aim of this research was to measure the ability of various spices to inhibit AGEs and to study the correlation of AGE inhibition with total phenolic (TP) content and antioxidant capacity. Fourteen spices commonly used in European cuisine were extracted with a 50% ethanol solution, and their water and total phenolic contents and antioxidant capacities were examined. Antioxidant capacity was evaluated using three methods: (1) Measurement of the radical scavenging ability of 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and (2) 2,2-diphenyl-1-picrylhydrazyl (DPPH●); and (3) photochemiluminescence (PCL) assay. Antiglycation properties were studied in vivo using two model systems: Bovine serum albumin-glucose (BSA-glucose) and bovine serum albumin-methylglyoxal (BSA-MGO). The most potent glycation inhibitors, according to the BSA-MGO assay, were star anise (88%), cinnamon (85%), allspice (81%), and cloves (79%), whereas in the BSA-glucose measurement, oregano was noted to be a very effective inhibitor of the glycation process. The ability to inhibit glycation was highly correlated with TP values in the BSA-MGO and BSA-glucose assay (r = 0.84 and 0.76, respectively). Our research showed the high antiglycation ability of cinnamon, cloves, and allspice, and we suggest, for the first time, that anise could also be considered a good glycation inhibitor.

Reduced Oxidative Stress in Hypoalbuminemic CAPD Patients

2000 ◽  
Vol 20 (3) ◽  
pp. 290-294 ◽  
Author(s):  
Soon Bae Kim ◽  
Won Seok Yang ◽  
Won Ki Min ◽  
Sang Koo Lee ◽  
Jung Sik Park
Keyword(s):  
Oxidative Stress ◽  
Cardiovascular Disease ◽  
Uric Acid ◽  
Serum Albumin ◽  
Antioxidant Capacity ◽  
Dialysis Patients ◽  
Albumin Infusion ◽  
Experimental Group ◽  
Normal Controls ◽  
Low Serum

Objective Albumin is one of the plasma antioxidants. The higher incidence of cardiovascular disease in dialysis patients with hypoalbuminemia may be related to decreased antioxidant capacity resulting from low serum albumin. We evaluated malondialdehyde (MDA), a marker of oxidative stress, and total antioxidant capacity (TAC) in relation to serum albumin in continuous ambulatory peritoneal dialysis (CAPD) patients. Patients and Methods In this cross-sectional study, we measured MDA, TAC, albumin, uric acid (another important plasma antioxidant), prealbumin, and C-reactive protein (CRP) in the blood of 66 CAPD patients and 30 normal controls. Twenty-two CAPD patients with albumin less than or equal to 3.5 g/dL were divided into two groups: In the experimental group ( n = 11), MDA, TAC, and CRP were measured before and after repeated infusion of 20% albumin 100 mL daily for 7 days. In the control group ( n = 11), the same parameters were measured without albumin infusion. Results CAPD patients had lower albumin and higher MDA, TAC, and uric acid than normal controls. There were positive correlations between albumin and TAC or uric acid in CAPD patients. Contrary to our expectation, there was also positive correlation between albumin and MDA in CAPD patients ( r = 0.37, p = 0.004). MDA showed a positive correlation with TAC. Uric acid was correlated with TAC. It did not, however, show correlation with MDA. Log CRP was negatively correlated with albumin, but did not show correlation with MDA or TAC. Prealbumin was correlated with MDA, TAC, albumin, and uric acid. Serum albumin in the experimental group increased from 2.5 ± 0.3 g/dL to 3.6 ± 0.2 g/dL ( p < 0.001) at the end of repeated infusions. No changes were seen in MDA, TAC, and CRP in either group. Conclusions The present study suggests that lipid peroxidation is increased in CAPD patients and that this is not due to weakening of antioxidant defenses of plasma. Increased TAC was mainly caused by a higher level of uric acid. Reduced, rather than increased, MDA in hypoalbuminemic patients and lack of effects of albumin infusion on levels of MDA suggest that the frequent occurrence of cardiovascular disease in dialysis patients with hypoalbuminemia is not due to the decrease in antioxidant capacity resulting from low serum albumin.

SELECTIVE CHEMILUMINESCENCE ANALYSIS OF AMADORI FORM OF GLYCATED HUMAN SERUM ALBUMIN

2000 ◽  
Vol 23 (20) ◽  
pp. 3119-3131 ◽  
Author(s):  
Toshihiko Hanai ◽  
Miyuki Uchida ◽  
Michiko Amao ◽  
Chiaki Ikeda ◽  
Kuniko Koizumi ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Chemiluminescence Analysis

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

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