The effects of experimental conditions of fluorescence quenching on the binding parameters of apigenin to bovine serum albumin by response surface methods

Luminescence ◽  
2013 ◽  
Vol 29 (4) ◽  
pp. 344-351 ◽  
Author(s):  
Lin Tang ◽  
Wanteng Jia ◽  
Dong Zhang
Keyword(s):  
Bovine Serum Albumin ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Response Surface ◽  
Bovine Serum ◽  
Binding Parameters ◽  
Experimental Conditions ◽  
Response Surface Methods

scholarly journals Evaluación del método dilución neutralización aplicado a un desinfectante según la Norma Técnica Colombiana 5473 de 2007

2012 ◽  
Vol 17 (1) ◽  
pp. 72
Author(s):  
Janeth Arias-Palacios ◽  
Libardo Hernandez-Esquivel ◽  
Juan Carlos Marín-Díaz ◽  
Natalia Navarro-Peña ◽  
Natalia Santos-Arévalo
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Key Words ◽  
Bactericidal Activity ◽  
Bovine Serum ◽  
Enterococcus Hirae ◽  
Experimental Conditions ◽  
Logarithmic Reduction

<strong>Objective</strong>. Evaluate the dilution-neutralization method proposed in the Colombian Technical Norm 5473/07, by using a gel, alcoholbased disinfectant. <strong>Materials and methods</strong>. This study was done using Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 6538, and Enterococcus hirae ATCC 10541 as the assay microorganisms. The study was carried out at 20±1°C as obligatory temperature and additionally at 36±1°C. Four contact times between microorganisms and the disinfectant were evaluated (0, 2, 5 and 10 minutes). The assay was done both under clean conditions (0.3 g/L of bovine serum albumin), and unclean conditions (3 g/L of bovine serum albumin and 3g/L of sheep erythrocytes). <strong>Results</strong>. The implementation of this method produced precise results in all of the six<br />repetitions used during the assay. The obtained results demonstrated a logarithmic reduction higher than five, demonstrating the bactericidal activity exerted by the disinfectant on the control microorganisms. The established experimental conditions and methodology did not affect negatively the growth of any of the strains of microorganisms. Similarly, the neutralizing used did not inhibit the development of the microorganisms of the assay.<strong> Conclusions</strong>. The method was verified by means of the fulfillment of the limits set by the rule. Our results suggest that the method evaluated by means of the implementation of the protocol established in the Colombian Technical Norm 5473/07, allows evaluating the effectiveness of a disinfectant under selected and controlled experimental conditions.<br /><br /><strong>Key words</strong>: disinfection, clean conditions, unclean conditions, dilution-neutralization method, logarithmic reduction.

Interaction between felodipine and bovine serum albumin: fluorescence quenching study

Luminescence ◽  
2009 ◽  
pp. n/a-n/a ◽  
Author(s):  
U. S. Mote ◽  
S. L. Bhattar ◽  
S. R. Patil ◽  
G. B. Kolekar
Keyword(s):  
Bovine Serum Albumin ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Bovine Serum

Interactions of different polyphenols with bovine serum albumin using fluorescence quenching and molecular docking

2012 ◽  
Vol 135 (4) ◽  
pp. 2418-2424 ◽  
Author(s):  
Mihaela Skrt ◽  
Evgen Benedik ◽  
Črtomir Podlipnik ◽  
Nataša Poklar Ulrih
Keyword(s):  
Molecular Docking ◽  
Bovine Serum Albumin ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Bovine Serum

scholarly journals In vitro Interactions of Secnidazole and Its Iron (II), Copper (II) Complexes with Bovine Serum Albumin by Fluorescence Quenching Method

2020 ◽  
Vol 23 (1) ◽  
pp. 1-9
Author(s):  
Md Jamal Hossain ◽  
Md Zakir Sultan ◽  
Mohammad A Rashid ◽  
Md Ruhul Kuddus
Keyword(s):  
Bovine Serum Albumin ◽  
Transition Metal ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Metal Complexes ◽  
Transition Metal Complexes ◽  
Bovine Serum ◽  
Spectroscopic Method ◽  
Van Der Waals Interactions

The current study was designed to investigate the interactions of an antimicrobial drug secnidazole and its two transition metal complexes with bovine serum albumin (BSA). The interactions of secnidazole and its both transition metal complexes were confirmed by the extingushing of fluorescence intensity of the protein. The fluorescence quenching of BSA by the drug and its both metal complexes showed a static quenching process and the reactions followed exothermic mechanism. The fluorescence spectroscopic method was utilized to evaluate the thermodynamic parameters like change of enthalpy (ΔH), entropy (ΔS) and Gibb’s free energy (ΔG) which indicated the bindings of the antimicrobial agent and its both metal chelates were hydrogen bonding and van der Waals interactions. The binding constant and the number of binding sites were also measured by double log plot that indicated the drug or its metal complexes bound with BSA at 1:1 ratio. Bangladesh Pharmaceutical Journal 23(1): 1-9, 2020

scholarly journals Interactions of rat α-foetoprotein with bilirubin

1979 ◽  
Vol 179 (3) ◽  
pp. 705-707 ◽  
Author(s):  
V Versée ◽  
A O Barel
Keyword(s):  
Absorption Spectrum ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Binding Site ◽  
Oxidation Rate ◽  
Bovine Serum ◽  
Bile Pigment ◽  
Binding Parameters ◽  
Enzymic Oxidation ◽  
Bilirubin Binding

The binding of bilirubin to rat-alpha-foetoprotein has been measured by changes in the absorption spectrum and by the appearance of two intense circular-dichroism bands. Furthermore binding of this bile pigment has been demonstrated by a decrease of its enzymic oxidation rate and by competition experiments with bovine serum albumin-agarose. The binding parameters have been determined as follows: n = 1.0 mol bound/mol of protein and Ka = 2.9 × 10(6) M-1. Competition of oestradiol for the bilirubin-binding site has not been established.

Probing the Interaction of Trans-resveratrol with Bovine Serum Albumin: A Fluorescence Quenching Study with Tachiya Model

2008 ◽  
Vol 18 (3-4) ◽  
pp. 671-678 ◽  
Author(s):  
J. B. Xiao ◽  
X. Q. Chen ◽  
X. Y. Jiang ◽  
M. Hilczer ◽  
M. Tachiya
Keyword(s):  
Bovine Serum Albumin ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Bovine Serum
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