Comment on “Does constructive neutral evolution play an important role in the origin of cellular complexity?” DOI 10.1002/bies.201100010

BioEssays ◽  
2011 ◽  
Vol 33 (6) ◽  
pp. 427-429 ◽  
Author(s):  
W. Ford Doolittle ◽  
Julius Lukeš ◽  
John M. Archibald ◽  
Patrick J. Keeling ◽  
Michael W. Gray
Keyword(s):  
Neutral Evolution ◽  
Constructive Neutral Evolution

scholarly journals The generality of Constructive Neutral Evolution

2018 ◽  
Vol 33 (1-2) ◽  
Author(s):  
T. D. P. Brunet ◽  
W. Ford Doolittle
Keyword(s):  
Neutral Evolution ◽  
Constructive Neutral Evolution

scholarly journals Constructive Neutral Evolution 20 Years Later

2021 ◽  
Vol 89 (3) ◽  
pp. 172-182 ◽  
Author(s):  
Sergio A. Muñoz-Gómez ◽  
Gaurav Bilolikar ◽  
Jeremy G. Wideman ◽  
Kerry Geiler-Samerotte
Keyword(s):  
Molecular Evolution ◽  
Neutral Evolution ◽  
Explanatory Framework ◽  
Evolution Of Complexity ◽  
Adaptive Processes ◽  
Constructive Neutral Evolution ◽  
Complex Features ◽  
Extensive List

AbstractEvolution has led to a great diversity that ranges from elegant simplicity to ornate complexity. Many complex features are often assumed to be more functional or adaptive than their simpler alternatives. However, in 1999, Arlin Stolzfus published a paper in the Journal of Molecular Evolution that outlined a framework in which complexity can arise through a series of non-adaptive steps. He called this framework Constructive Neutral Evolution (CNE). Despite its two-decade-old roots, many evolutionary biologists still appear to be unaware of this explanatory framework for the origins of complexity. In this perspective piece, we explain the theory of CNE and how it changes the order of events in narratives that describe the evolution of complexity. We also provide an extensive list of cellular features that may have become more complex through CNE. We end by discussing strategies to determine whether complexity arose through neutral or adaptive processes.

scholarly journals RGB-Marking to Identify Patterns of Selection and Neutral Evolution in Human Osteosarcoma Models

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2003
Author(s):  
Stefano Gambera ◽  
Ana Patiño-Garcia ◽  
Arantzazu Alfranca ◽  
Javier Garcia-Castro
Keyword(s):  
Clonal Selection ◽  
Clonal Evolution ◽  
Phylogenetic Reconstruction ◽  
Experimental Models ◽  
Neutral Evolution ◽  
Immunodeficient Mice ◽  
Nsg Mice ◽  
Clonal Development ◽  
Clonal Composition

Osteosarcoma (OS) is a highly aggressive tumor characterized by malignant cells producing pathologic bone; the disease presents a natural tendency to metastasize. Genetic studies indicate that the OS genome is extremely complex, presenting signs of macro-evolution, and linear and branched patterns of clonal development. However, those studies were based on the phylogenetic reconstruction of next-generation sequencing (NGS) data, which present important limitations. Thus, testing clonal evolution in experimental models could be useful for validating this hypothesis. In the present study, lentiviral LeGO-vectors were employed to generate colorimetric red, green, blue (RGB)-marking in murine, canine, and human OS. With this strategy, we studied tumor heterogeneity and the clonal dynamics occurring in vivo in immunodeficient NOD.Cg-Prkdcscid-Il2rgtm1Wjl/SzJ (NSG) mice. Based on colorimetric label, tumor clonal composition was analyzed by confocal microscopy, flow cytometry, and different types of supervised and unsupervised clonal analyses. With this approach, we observed a consistent reduction in the clonal composition of RGB-marked tumors and identified evident clonal selection at the first passage in immunodeficient mice. Furthermore, we also demonstrated that OS could follow a neutral model of growth, where the disease is defined by the coexistence of different tumor sub-clones. Our study demonstrates the importance of rigorous testing of the selective forces in commonly used experimental models.

Sign in / Sign up

Export Citation Format

Share Document