Efficacy of 50 Hz electromagnetic fields on human epidermal stem cell transplantation seeded in collagen sponge scaffolds for wound healing in a murine model

2017 ◽  
Vol 38 (3) ◽  
pp. 204-212 ◽  
Author(s):  
Wen-Fang Bai ◽  
Wei-Cheng Xu ◽  
Hong-Xiang Zhu ◽  
Hong Huang ◽  
Bo Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Electromagnetic Fields ◽  
Murine Model ◽  
Collagen Sponge ◽  
Epidermal Stem Cell

CCR7 impairs hematopoiesis after hematopoietic stem cell transplantation increasing susceptibility to invasive aspergillosis

Blood ◽  
2010 ◽  
Vol 116 (24) ◽  
pp. 5383-5393 ◽  
Author(s):  
Adam J. Hartigan ◽  
Lara E. Kallal ◽  
Cory M. Hogaboam
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell Transplantation ◽  
Stem Cell Transplantation ◽  
Invasive Aspergillosis ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Murine Model ◽  
Cell Expansion ◽  
Opportunistic Infections ◽  
Hematopoietic Stem

Abstract Hematopoietic stem cell transplantation (HSCT) is limited by patient susceptibility to opportunistic infections. One of the most devastating infections after HSCT is invasive aspergillosis (IA), a life-threatening disease caused by Aspergillus fumigatus. Transplantation of hematopoietic stem cells (HSCs) and myeloid progenitor cells (MPCs) has been shown to mediate protection against IA, but little is known about the factors that regulate HSC and MPC cell expansion after transplantation. Herein, we investigated the role of CCR7 in a murine model of IA after combined HSC and MPC transplantation into lethally irradiated wild-type (WT) mice. Nonirradiated CCR7−/− mice had expanded populations of HSCs in the bone marrow and spleen, compared with WT mice. Irradiated WT mice reconstituted with CCR7−/− HSCs and MPCs had increased survival, decreased fungal burden, and enhanced myeloid leukocyte numbers during IA, compared with WT controls. In addition, WT mice reconstituted with WT HSCs and MPCs and treated with anti-CCR7 exhibited accelerated myeloid cell expansion similar to that observed in CCR7−/−→WT chimeras. Thus, removal of the inhibitory effects of CCR7 through genetic alteration or ligand immunoneutralization enhanced myeloid reconstitution, thereby accelerating fungal clearance in a murine model of IA.

scholarly journals Cell competition between wild-type and JAK2V617F mutant cells prevents disease relapse after stem cell transplantation in a murine model of myeloproliferative neoplasm

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Haotian Zhang ◽  
Melissa Castiglione ◽  
Lei Zheng ◽  
Huichun Zhan
Keyword(s):  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Murine Model ◽  
Molecular Mechanisms ◽  
Myeloproliferative Neoplasms ◽  
Disease Relapse ◽  
Wild Type ◽  
Cell Competition ◽  
Mutant Cells

AbstractDisease relapse after allogeneic stem cell transplantation is a major cause of treatment-related morbidity and mortality in patients with myeloproliferative neoplasms (MPNs). The cellular and molecular mechanisms for MPN relapse are not well understood. Here, we established a murine model of MPN relapse, in which ~ 60% of the MPN recipient mice develop disease relapse after receiving stem cell transplantation with wild-type marrow donor. Using this model, we find that impaired wild-type cell function is associated with MPN disease relapse. We also show that competition between wild-type and JAK2V617F mutant cells can modulate the immune cell composition and PD-L1 expression induced by the JAK2V617F oncogene. These results suggest that cell competition between wild-type donor cells and JAK2V617F mutant recipient cells can prevent MPN disease relapse after stem cell transplantation.

scholarly journals A murine model demonstrating reversal of structural and functional correlates of cirrhosis with progenitor cell transplantation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mark D. Muthiah ◽  
Daniel Q. Huang ◽  
Lei Zhou ◽  
Nur Halisah Jumat ◽  
Mahesh Choolani ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Stem Cell ◽  
Liver Disease ◽  
Stem Cell Transplantation ◽  
Cell Transplantation ◽  
Murine Model ◽  
Fetal Liver ◽  
Decompensated Liver Disease ◽  
Human Fetal Liver

Abstract Development of cell transplantation for treating liver cirrhosis hinges critically on the availability of animal models for studying human stem cell transplantation. We report an immune-permissive murine model of liver cirrhosis with full clinical correlates of decompensated liver disease, and allows testing efficacy of stem cell transplantation. Liver cirrhosis was induced in Nod-scid gamma(NSG) mice with oral thioacetamide(TA) and compared to controls over 12 months. 4 month TA treated cirrhotic mice were then transplanted intrasplenically with 2million human fetal liver progenitor cells(HFH) and compared with cirrhotic controls 2 months after transplantation. NSG-TA mice developed shrunken and nodular livers with histological evidence of fibrosis as compared to controls. This was associated with evidence of worsening decompensated liver disease, with jaundice, hypoalbuminemia, coagulopathy, and encephalopathy in NSG-TA mice. Transplantation of HFH resulted in improvement in both fibrosis and markers of decompensated liver disease. We have demonstrated that NSG-TA mice can recapitulate the full clinical picture of structural and functional cirrhosis, both of which can be improved by transplantation of human fetal liver cells. This model serves as a valuable tool for validation of in vivo liver stem cell transplantation and opens up opportunities for studying the mechanism how stem cells reverse fibrosis.

Sign in / Sign up

Export Citation Format

Share Document