Extremely low frequency electromagnetic fields (ELF-EMFs) induce in vitro angiogenesis process in human endothelial cells

2008 ◽  
Vol 29 (8) ◽  
pp. 640-648 ◽  
Author(s):  
Simona Delle Monache ◽  
Riccardo Alessandro ◽  
Roberto Iorio ◽  
Giancaterino Gualtieri ◽  
Rosella Colonna
Keyword(s):  
Endothelial Cells ◽  
Electromagnetic Fields ◽  
Low Frequency ◽  
Human Endothelial Cells ◽  
Extremely Low Frequency ◽  
Angiogenesis Process ◽  
In Vitro Angiogenesis

scholarly journals Human Gingival Fibroblasts Exposed to Extremely Low-Frequency Electromagnetic Fields: In Vitro Model of Wound-Healing Improvement

2019 ◽  
Vol 20 (9) ◽  
pp. 2108 ◽  
Author(s):  
Erica Costantini ◽  
Bruna Sinjari ◽  
Chiara D’Angelo ◽  
Giovanna Murmura ◽  
Marcella Reale ◽  
...  
Keyword(s):  
Wound Healing ◽  
Electromagnetic Field ◽  
Electromagnetic Fields ◽  
Wound Repair ◽  
Healing Process ◽  
Low Frequency ◽  
Gingival Fibroblasts ◽  
Field Exposure ◽  
Extremely Low Frequency

Several clinical studies have suggested the impact of sinusoidal and pulsed electromagnetic fields in quickening wound repair processes and tissue regeneration. The clinical use of extremely low-frequency electromagnetic fields could represent a novel frontier in tissue repair and oral health, with an interesting clinical perspective. The present study aimed to evaluate the effect of an extremely low-frequency sinusoidal electromagnetic field (SEMF) and an extremely low-frequency pulsed electromagnetic field (PEMF) with flux densities of 1 mT on a model of oral healing process using gingival fibroblasts. An in vitro mechanical injury was produced to evaluate wound healing, migration, viability, metabolism, and the expression of selected cytokines and protease genes in fibroblasts exposed to or not exposed to the SEMF and the PEMF. Interleukin 6 (IL-6), transforming growth factor beta 1 (TGF-β), metalloproteinase 2 (MMP-2), monocyte chemoattractant protein 1 (MCP-1), inducible nitric oxide synthase (iNOS), and heme oxygenase 1 (HO-1) are involved in wound healing and tissue regeneration, favoring fibroblast proliferation, chemotaxis, and activation. Our results show that the exposure to each type of electromagnetic field increases the early expression of IL-6, TGF-β, and iNOS, driving a shift from an inflammatory to a proliferative phase of wound repair. Additionally, a later induction of MMP-2, MCP-1, and HO-1 was observed after electromagnetic field exposure, which quickened the wound-healing process. Moreover, electromagnetic field exposure influenced the proliferation, migration, and metabolism of human gingival fibroblasts compared to sham-exposed cells. This study suggests that exposure to SEMF and PEMF could be an interesting new non-invasive treatment option for wound healing. However, additional studies are needed to elucidate the best exposure conditions to provide the desired in vivo treatment efficacy.

Brassinosteroids inhibit in vitro angiogenesis in human endothelial cells

Steroids ◽  
2012 ◽  
Vol 77 (13) ◽  
pp. 1502-1509 ◽  
Author(s):  
Lucie Rárová ◽  
Stefan Zahler ◽  
Johanna Liebl ◽  
Vladimír Kryštof ◽  
David Sedlák ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Endothelial Cells ◽  
In Vitro Angiogenesis

Effect of extremely low frequency electromagnetic fields on thromboxane B2-release by ionophore-stimulated neutrophils in vitro

1984 ◽  
Vol 27 ◽  
pp. 96 ◽  
Author(s):  
P. Conti ◽  
G.E. Gigante ◽  
M.G. Cifone ◽  
E. Alesse ◽  
G. Ianni ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Low Frequency ◽  
Thromboxane B2 ◽  
Extremely Low Frequency

scholarly journals 0383 : In vitro 3D model of in vitro angiogenesis using human endothelial cells and pericytes

2015 ◽  
Vol 7 (2) ◽  
pp. 148
Author(s):  
Ester Garcia-Valiente ◽  
Elsa Vanhecke ◽  
Laurent Muller ◽  
Bruno Peault ◽  
Germain Stéphane
Keyword(s):  
Endothelial Cells ◽  
3D Model ◽  
Human Endothelial Cells ◽  
In Vitro Angiogenesis

Extremely low-frequency electromagnetic fields promote in vitro neurogenesis via upregulation of Cav1-channel activity

2008 ◽  
Vol 215 (1) ◽  
pp. 129-139 ◽  
Author(s):  
Roberto Piacentini ◽  
Cristian Ripoli ◽  
Daniele Mezzogori ◽  
Gian Battista Azzena ◽  
Claudio Grassi
Keyword(s):  
Electromagnetic Fields ◽  
Low Frequency ◽  
Channel Activity ◽  
Extremely Low Frequency

Therapeutic Application of Musically Modulated Electromagnetic Fields in the Treatment of Musculoskeletal Disorders

2012 ◽  
Vol 10 (3) ◽  
pp. 257-267
Author(s):  
C. Corallo ◽  
M. Rigato ◽  
E. Battisti ◽  
A. Albanese ◽  
S. Gonnelli ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Musculoskeletal Disorders ◽  
Electromagnetic Fields ◽  
Experimental Studies ◽  
Low Frequency ◽  
Experimental Point ◽  
Therapeutic Application ◽  
Extremely Low Frequency ◽  
Positive Effects

Different studies have demonstrated the efficacy of extremely low frequency electromagnetic fields (ELF EMFs) in the treatment of pain. In particular, the positive effects of ELF EMFs seems to depend on their respective codes, such as frequency, intensity and waveform, even if the exact mechanism of interaction is still debated. The most commonly used for extremely low frequency magnetotherapy is a 100Hz sinusoidal field (ELF) with a mean of induction of few Gauss. This article reviews the therapeutic application of a musically modulated electromagnetic field (TAMMEF), a new-generation of electromagnetic field used for extremely low frequency magnetotherapy characterized by variable frequencies, intensities and waveforms. Both clinical and experimental studies, performed by authors of the present review, have demonstrated the efficacy of ELF and the new TAMMEF systems in several musculoskeletal disorders such as osteoarthritis, rheumatoid arthritis, carpal tunnel syndrome, shoulder periarthritis and cervical spondylosis. Moreover, it has been demonstrated that ELF and TAMMEF systems are not only effective, but also safe, from clinical and experimental point of view. In fact, clinical trials did not reported any undesired side effect, while in vitro studies showed that ELF EMFs did not induce uncontrolled cell proliferation, did not affect cell viability and did not induce apoptosis. With their efficacy and safety, ELF and even more the new TAMMEF systems represent a valid complementary or alternative treatment to standard pharmacological therapies in reducing both pain and inflammation of patients affected by musculoskeletal disorders.

scholarly journals Alterations in Adenylate Kinase Activity in Human PBMCs after In Vitro Exposure to Electromagnetic Field: Comparison between Extremely Low Frequency Electromagnetic Field (ELF) and Therapeutic Application of a Musically Modulated Electromagnetic Field (TAMMEF)

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Antonietta Albanese ◽  
Emilio Battisti ◽  
Daniela Vannoni ◽  
Emilia Aceto ◽  
Gianmichele Galassi ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Electromagnetic Fields ◽  
Adenylate Kinase ◽  
Kinase Activity ◽  
Low Frequency ◽  
Purine Metabolism ◽  
Electrical Charge ◽  
Therapeutic Application ◽  
Extremely Low Frequency

This study investigated the effects of electromagnetic fields on enzymes involved in purine metabolism in human peripheral blood mononuclear cells in vitro. Cells were obtained from 20 volunteers. We tested both low-energy, extremely low frequency (ELF; 100-Hz) electromagnetic fields and the Therapeutic Application of Musically Modulated Electromagnetic Fields (TAMMEFs); the latter is characterized by variable frequencies, intensities, and wave shapes. Adenylate kinase activity was increased after ELF field exposure but decreased slightly after TAMMEF exposure. Neither of the two electromagnetic field affected the activities of the purine metabolism enzymes ecto-5′-nucleotidase, adenosine deaminase, and adenosine kinase. We concluded that ELF fields may influence cellular electrical charge stability; stimulation of adenylate kinase activity could restore the cell to a state of equilibrium. In contrast, TAMMEF fields may be useful for maintaining and regulating the cellular electrical charge.

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