Mechanisms controlling human endothelial lumen formation and tube assembly in three-dimensional extracellular matrices

2007 ◽  
Vol 81 (4) ◽  
pp. 270-285 ◽  
Author(s):  
George E. Davis ◽  
Wonshill Koh ◽  
Amber N. Stratman
Keyword(s):  
Three Dimensional ◽  
Extracellular Matrices ◽  
Lumen Formation ◽  
Tube Assembly

scholarly journals Molecular Mechanisms Controlling Vascular Lumen Formation in Three-Dimensional Extracellular Matrices

2012 ◽  
Vol 195 (1-2) ◽  
pp. 122-143 ◽  
Author(s):  
Anastasia Sacharidou ◽  
Amber N. Stratman ◽  
George E. Davis
Keyword(s):  
Molecular Mechanisms ◽  
Three Dimensional ◽  
Extracellular Matrices ◽  
Lumen Formation ◽  
Vascular Lumen

scholarly journals The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices

2002 ◽  
Vol 115 (6) ◽  
pp. 1123-1136 ◽  
Author(s):  
Kayla J. Bayless ◽  
George E. Davis
Keyword(s):  
Rho Gtpases ◽  
Fluorescent Protein ◽  
Three Dimensional ◽  
Dominant Negative ◽  
Extracellular Matrices ◽  
Wild Type ◽  
Lumen Formation ◽  
Clostridium Difficile Toxin B ◽  
Green Fluorescent ◽  
Intracellular Vacuole

Here we show a requirement for the Cdc42 and Rac1 GTPases in endothelial cell (EC) morphogenesis in three-dimensional extracellular matrices. Cdc42 and Rac1 specifically regulate EC intracellular vacuole and lumen formation in both collagen and fibrin matrices. Clostridium difficile toxin B(which blocks all three Rho GTPases) completely inhibited the ability of ECs to form both vacuoles and lumens, whereas C3 transferase, a selective inhibitor of Rho, did not. Expression of either dominant-negative (N17) or constitutively active (V12) Cdc42 using recombinant adenoviruses dramatically inhibited EC vacuole and lumen formation in both collagen and fibrin matrices. Both vacuole and lumen formation initiated in ECs expressing dominant-negative(N17) Rac1 but later collapsed, indicating a role for Rac1 during later stages of vessel development. Analysis of cultures using confocal microscopy revealed green fluorescent protein-V12Rac1, -Rac1 wild-type and -Cdc42 wild-type chimeric proteins targeted to intracellular vacuole membranes during the lumen formation process. Also, expression of the verprolin-cofilin-acidic domain of N-WASP, a downstream Cdc42 effector, in ECs completely interfered with vacuole and lumen formation. These results collectively reveal a novel role for Cdc42 and Rac1 in the process of EC vacuole and lumen formation in three-dimensional extracellular matrices.

scholarly journals Functional Roles for PECAM-1 (CD31) and VE-Cadherin (CD144. in Tube Assembly and Lumen Formation in Three-Dimensional Collagen Gels

1999 ◽  
Vol 155 (3) ◽  
pp. 887-895 ◽  
Author(s):  
Suya Yang ◽  
Jennifer Graham ◽  
Jeanne W. Kahn ◽  
Eric A. Schwartz ◽  
Mary E. Gerritsen
Keyword(s):  
Three Dimensional ◽  
Collagen Gels ◽  
Lumen Formation ◽  
Functional Roles ◽  
Tube Assembly

098
Cytokine Dynamics in Three-Dimensional Extracellular Matrices

2008 ◽  
Vol 13 (2) ◽  
pp. A28-A48
Author(s):  
N.R. Washburn ◽  
M.D. Weir ◽  
K.M. Yamada
Keyword(s):  
Three Dimensional ◽  
Extracellular Matrices
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