Inflammatory Protein Expression in Adolescent and Adult Offspring of Type 1 Diabetic Mice

2012 ◽  
Vol 95 (5) ◽  
pp. 376-378 ◽  
Author(s):  
Daniel Dowling ◽  
Niamh Corrigan ◽  
Paul Downey ◽  
Fionnuala M. McAuliffe
Keyword(s):  
Protein Expression ◽  
Adult Offspring ◽  
Diabetic Mice ◽  
Inflammatory Protein

P0979BENEFICIAL EFFECT OF CHLOROQUINE AND AMODIAQUINE ON DIABETIC TUBULOPATHY BY ATTENUATING MITOCHONDRIAL NOX4 AND ENDOPLASMIC RETICULUM STRESS

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
So-Young Lee ◽  
Dong Ho Yang ◽  
Yu Ho Lee ◽  
Mi Jung Lee ◽  
Hyung-Jong Kim ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Protein Expression ◽  
Er Stress ◽  
High Glucose ◽  
Kidney Tissue ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Diabetic Mice ◽  
Chronic Hyperglycemia ◽  
Inflammatory Protein ◽  
Diabetic Tubulopathy

Abstract Background and Aims Oxidative stress induced by chronic hyperglycemia is recognized as a significant mechanistic contributor to the development of diabetic kidney disease (DKD). Nonphagocytic nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4) is a major source of reactive oxygen species (ROS) in many cell types and in the kidney tissue of diabetic animals. We designed this study to explore the therapeutic potential of chloroquine and amodiaquine for inhibiting mitochondrial Nox4 and diabetic tubular injury. Method Human renal proximal tubular epithelial cells (hRPTCs) were cultured in high-glucose media (30 mM D-glucose), and diabetes was induced with streptozotocin (STZ, 50 mg/kg i.p. for 5 days) in male C57/BL6J mice. Chloroquine and amodiaquine were administered to the mice via intraperitoneal injection for 14 weeks. Results Chloroquine and amodiaquine inhibited mitochondrial Nox4 and increased mitochondrial mass in hRPTCs under high-glucose conditions. Reduced mitochondrial ROS production after treatment with the drugs resulted in decreased endoplasmic reticulum (ER) stress, suppressed inflammatory protein expression and reduced cell apoptosis in hRPTCs under high-glucose conditions. Notably, chloroquine and amodiaquine treatment diminished Nox4 activation and ER stress in the kidneys of STZ-induced diabetic mice. In addition, we observed attenuated inflammatory protein expression and albuminuria in STZ-induced diabetic mice after chloroquine and amodiaquine treatment. Conclusion We substantiated the protective actions of chloroquine and amodiaquine in diabetic tubulopathy associated with reduced mitochondrial Nox4 activation and ER stress alleviation. Further studies exploring the roles of mitochondrial Nox4 in the pathogenesis of DKD could suggest new therapeutic targets for patients with DKD. Figure None

scholarly journals Ptpn22 and Cd2 Variations Are Associated with Altered Protein Expression and Susceptibility to Type 1 Diabetes in Nonobese Diabetic Mice

2015 ◽  
Vol 195 (10) ◽  
pp. 4841-4852 ◽  
Author(s):  
Heather I. Fraser ◽  
Sarah Howlett ◽  
Jan Clark ◽  
Daniel B. Rainbow ◽  
Stephanie M. Stanford ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Protein Expression ◽  
Diabetic Mice ◽  
Nonobese Diabetic ◽  
Altered Protein ◽  
Nonobese Diabetic Mice

463-P: Linagliptin Ameliorated Heart Damage Associated with the Suppression of Necroptosis in Type 1 Diabetic Mice

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 463-P
Author(s):  
TARO HIRAI ◽  
YUTA TAKAGAKI ◽  
KEIZO KANASAKI ◽  
DAISUKE KOYA
Keyword(s):  
Diabetic Mice ◽  
Heart Damage

Hydrogen sulfide alleviates the anxiety-like and depressive-like behaviors of type 1 diabetic mice via inhibiting inflammation and ferroptosis

Life Sciences ◽  
2021 ◽  
pp. 119551
Author(s):  
Yi Wang ◽  
Shengwen Wang ◽  
Yu Xin ◽  
Jinyue Zhang ◽  
Shaofan Wang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Diabetic Mice

scholarly journals Low Incidence of Spontaneous Type 1 Diabetes in Non-Obese Diabetic Mice Raised on Gluten-Free Diets Is Associated with Changes in the Intestinal Microbiome

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e78687 ◽  
Author(s):  
Eric V. Marietta ◽  
Andres M. Gomez ◽  
Carl Yeoman ◽  
Ashenafi Y. Tilahun ◽  
Chad R. Clark ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Intestinal Microbiome ◽  
Gluten Free ◽  
Diabetic Mice ◽  
Low Incidence

Resveratrol inhibits macrophage infiltration of pancreatic islets in streptozotocin-induced type 1 diabetic mice via attenuation of the CXCL16/NF-κΒ p65 signaling pathway

Life Sciences ◽  
2021 ◽  
Vol 272 ◽  
pp. 119250
Author(s):  
Mostafa A. Darwish ◽  
Amira M. Abo-Youssef ◽  
Basim A.S. Messiha ◽  
Ali A. Abo-Saif ◽  
Mohamed S. Abdel-Bakky
Keyword(s):  
Pancreatic Islets ◽  
Signaling Pathway ◽  
Macrophage Infiltration ◽  
Diabetic Mice
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