scholarly journals Retinoic acid signaling regulates Sonic hedgehog and bone morphogenetic protein signalings during genital tubercle development

2011 ◽  
pp. n/a-n/a ◽  
Author(s):  
Liqing Liu ◽  
Kentaro Suzuki ◽  
Naomi Nakagata ◽  
Kenichiro Mihara ◽  
Daisuke Matsumaru ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Bone Morphogenetic Protein ◽  
Sonic Hedgehog ◽  
Retinoic Acid Signaling ◽  
Morphogenetic Protein ◽  
Genital Tubercle

scholarly journals Bone morphogenetic protein and retinoic acid signaling cooperate to induce osteoblast differentiation of preadipocytes

2002 ◽  
Vol 159 (1) ◽  
pp. 135-146 ◽  
Author(s):  
Jeremy Skillington ◽  
Lisa Choy ◽  
Rik Derynck
Keyword(s):  
Retinoic Acid ◽  
Bone Morphogenetic Protein ◽  
Osteoblast Differentiation ◽  
Adipogenic Differentiation ◽  
Osteoblastic Differentiation ◽  
Mesenchymal Cells ◽  
Bmp Signaling ◽  
Retinoic Acid Signaling ◽  
Morphogenetic Protein ◽  
Adipose Conversion

Mesenchymal cells can differentiate into osteoblasts, adipocytes, myoblasts, or chondroblasts. Whether mesenchymal cells that have initiated differentiation along one lineage can transdifferentiate into another is largely unknown. Using 3T3-F442A preadipocytes, we explored whether extracellular signals could redirect their differentiation from adipocyte into osteoblast. 3T3-F442A cells expressed receptors and Smads required for bone morphogenetic protein (BMP) signaling. BMP-2 increased proliferation and induced the early osteoblast differentiation marker alkaline phosphatase, yet only mildly affected adipogenic differentiation. Retinoic acid inhibited adipose conversion and cooperated with BMP-2 to enhance proliferation, inhibit adipogenesis, and promote early osteoblastic differentiation. Expression of BMP-RII together with BMP-RIA or BMP-RIB suppressed adipogenesis of 3T3-F442A cells and promoted full osteoblastic differentiation in response to retinoic acid. Osteoblastic differentiation was characterized by induction of cbfa1, osteocalcin, and collagen I expression, and extracellular matrix calcification. These results indicate that 3T3-F442A preadipocytes can be converted into fully differentiated osteoblasts in response to extracellular signaling cues. Furthermore, BMP and retinoic acid signaling cooperate to stimulate cell proliferation, repress adipogenesis, and promote osteoblast differentiation. Finally, BMP-RIA and BMP-RIB induced osteoblast differentiation and repressed adipocytic differentiation to a similar extent.

scholarly journals Sonic Hedgehog Signaling Is Required for Cyp26 Expression during Embryonic Development

2019 ◽  
Vol 20 (9) ◽  
pp. 2275 ◽  
Author(s):  
Maha El Shahawy ◽  
Claes-Göran Reibring ◽  
Kristina Hallberg ◽  
Cynthia L. Neben ◽  
Pauline Marangoni ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Sonic Hedgehog ◽  
Congenital Malformations ◽  
Hedgehog Signaling ◽  
Retinoic Acid Signaling ◽  
Loss Of Function ◽  
Sonic Hedgehog Signaling ◽  
Secondary Palate ◽  
Experimental Approaches ◽  
Genital Tubercle

Deciphering how signaling pathways interact during development is necessary for understanding the etiopathogenesis of congenital malformations and disease. In several embryonic structures, components of the Hedgehog and retinoic acid pathways, two potent players in development and disease are expressed and operate in the same or adjacent tissues and cells. Yet whether and, if so, how these pathways interact during organogenesis is, to a large extent, unclear. Using genetic and experimental approaches in the mouse, we show that during development of ontogenetically different organs, including the tail, genital tubercle, and secondary palate, Sonic hedgehog (SHH) loss-of-function causes anomalies phenocopying those induced by enhanced retinoic acid signaling and that SHH is required to prevent supraphysiological activation of retinoic signaling through maintenance and reinforcement of expression of the Cyp26 genes. Furthermore, in other tissues and organs, disruptions of the Hedgehog or the retinoic acid pathways during development generate similar phenotypes. These findings reveal that rigidly calibrated Hedgehog and retinoic acid activities are required for normal organogenesis and tissue patterning.

Bone morphogenetic protein/retinoic acid inducible neural-specific protein (brinp) expression during Danio rerio development

2015 ◽  
Vol 18 (1-2) ◽  
pp. 37-43 ◽  
Author(s):  
Aminah Giousoh ◽  
Raquel Vaz ◽  
Robert J. Bryson-Richardson ◽  
James C. Whisstock ◽  
Heather Verkade ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Bone Morphogenetic Protein ◽  
Danio Rerio ◽  
Specific Protein ◽  
Morphogenetic Protein

scholarly journals BMP action in skeletogenesis involves attenuation of retinoid signaling

2006 ◽  
Vol 174 (1) ◽  
pp. 101-113 ◽  
Author(s):  
Lisa M. Hoffman ◽  
Kamal Garcha ◽  
Konstantina Karamboulas ◽  
Matthew F. Cowan ◽  
Linsay M. Drysdale ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Organ Culture ◽  
Signaling Pathways ◽  
Bone Morphogenetic Protein ◽  
Signaling Pathway ◽  
Signaling Molecules ◽  
Bmp Signaling ◽  
Retinoid Signaling ◽  
Morphogenetic Protein ◽  
Bona Fide

The bone morphogenetic protein (BMP) and growth and differentiation factor (GDF) signaling pathways have well-established and essential roles within the developing skeleton in coordinating the formation of cartilaginous anlagen. However, the identification of bona fide targets that underlie the action of these signaling molecules in chondrogenesis has remained elusive. We have identified the gene for the retinoic acid (RA) synthesis enzyme Aldh1a2 as a principal target of BMP signaling; prochondrogenic BMPs or GDFs lead to attenuation of Aldh1a2 expression and, consequently, to reduced activation of the retinoid signaling pathway. Consistent with this, antagonism of retinoid signaling phenocopies BMP4 action, whereas RA inhibits the chondrogenic stimulatory activity of BMP4. BMP4 also down-regulates Aldh1a2 expression in organ culture and, consistent with this, Aldh1a2 is actively excluded from the developing cartilage anlagens. Collectively, these findings provide novel insights into BMP action and demonstrate that BMP signaling governs the fate of prechondrogenic mesenchyme, at least in part, through regulation of retinoid signaling.

Differentiation of human pluripotent teratocarcinoma stem cells induced by bone morphogenetic protein-2

1998 ◽  
Vol 10 (8) ◽  
pp. 551 ◽  
Author(s):  
Martin F. Pera ◽  
Daniella Herszfeld
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Retinoic Acid ◽  
Cell Line ◽  
Bone Morphogenetic Protein ◽  
Morphological Changes ◽  
Bone Morphogenetic Protein 2 ◽  
Stem Cell Markers ◽  
Potent Inducer ◽  
Morphogenetic Protein

Pluripotent human teratocarcinoma stem cells cultured in vitro provide a resource for the study of early embryonic development in man, as well as a means for discovery of novel factors controlling cell differentiation and commitment. We previously reported that the human teratocarcinoma stem cell line GCT 27X-1 could be induced to differentiate into an endodermal progenitor cell by treatment with high doses of retinoic acid. A search for polypeptide inducers of differentiation in this system has identified bone morphogenetic protein-2 (BMP-2) as a potent inducer of differentiation. In cell line GCT 27X-1, treatment with BMP-2 reduces proliferation, induces morphological changes similar to obtained following treatment with retinoic acid, and causes a decrease in the expression of transcripts for the stem cell markers CD30 and Oct-4. Preliminary immunochemical studies indicate that the differentiated cells produced by BMP-2 are endodermal precursors with a pattern of marker expression similar to that found in retinoic acid treated cells. Models of endoderm differentiation in humans will be useful for identifying the molecules which mediate cell interactions in development, and in achieving directed differentiation of cells for use in transplantation.

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