Characterization of human cytochrome P450 enzymes involved in thein vitro metabolism of perospirone

2005 ◽  
Vol 26 (2) ◽  
pp. 59-65 ◽  
Author(s):  
Atsushi Kitamura ◽  
Yoshiko Mizuno ◽  
Kiyoshi Natsui ◽  
Masashi Yabuki ◽  
Setsuko Komuro ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome

Characterization of Diuron N-Demethylation by Mammalian Hepatic Microsomes and cDNA-Expressed Human Cytochrome P450 Enzymes

2007 ◽  
Vol 35 (9) ◽  
pp. 1634-1641 ◽  
Author(s):  
Khaled Abass ◽  
Petri Reponen ◽  
Miia Turpeinen ◽  
Jorma Jalonen ◽  
Olavi Pelkonen
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Hepatic Microsomes ◽  
Human Cytochrome

Characterization of human cytochrome P450 enzymes involved in the metabolism of cyamemazine

2007 ◽  
Vol 32 (4-5) ◽  
pp. 357-366 ◽  
Author(s):  
Christophe Arbus ◽  
Amine Benyamina ◽  
Pierre-Michel Llorca ◽  
Franck Baylé ◽  
Norbert Bromet ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome

Pharmacokinetic characterization of carnosol from rosemary (Salvia Rosmarinus) in male C57BL/6 mice and inhibition profile in human cytochrome P450 enzymes

2021 ◽  
pp. 115729
Author(s):  
Bhaskar Vemu ◽  
Restituo Tocmo ◽  
Mirielle C. Nauman ◽  
Stephanie A. Flowers ◽  
Jacob P. Veenstra ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome

scholarly journals Highly Miniaturized Formats for In Vitro Drug Metabolism Assays Using Vivid® Fluorescent Substrates and Recombinant Human Cytochrome P450 Enzymes

2005 ◽  
Vol 10 (1) ◽  
pp. 56-66 ◽  
Author(s):  
Olga V. Trubetskoy ◽  
Jasmin R. Gibson ◽  
Bryan D. Marks
Keyword(s):  
Cytochrome P450 ◽  
High Throughput Screening ◽  
Cytochrome P450 Enzymes ◽  
Fluorogenic Substrates ◽  
Detailed Characterization ◽  
Human Cytochrome ◽  
Screening Assays ◽  
Well Assay

Highly miniaturized P450 screening assays designed to enable facile analysis of P450 drug interactions in a 1536-well plate format with the principal human cytochrome P450 enzymes (CYP3A4, 2D6, 2C9, 2C19, and 1A2) and Vivid® fluorogenic substrates were developed. The detailed characterization of the assays included stability, homogeneity, and reproducibility of the recombinant P450 enzymes and the kinetic parameters of their reactions with Vivid® fluorogenic substrates, with a focus on the specific characteristics of each component that enable screening in a low-volume 1536-well plate assay format. The screening assays were applied for the assessment of individual cytochrome P450 inhibition profiles with a panel of selected assay modifiers, including isozyme-specific substrates and inhibitors. IC50 values obtained for the modifiers in 96- and 1536-well plate formats were similar and comparable with values obtained in assays with conventional substrates. An overall examination of the 1536-well assay statistics, such as signal-to-background ratio and Z′ factor, demonstrated that these assays are a robust, successful, and reliable tool to screen for cytochrome P450 metabolism and inhibition in an ultra-high-throughput screening format. ( Journal of Biomolecular Screening 2005:56-66)

Electron transfer in human cytochrome P450 reductase

2003 ◽  
Vol 31 (3) ◽  
pp. 497-501 ◽  
Author(s):  
A. Gutierrez ◽  
A. Grunau ◽  
M. Paine ◽  
A.W. Munro ◽  
C.R. Wolf ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Cytochrome P450 ◽  
Site Directed Mutagenesis ◽  
Cytochrome P450 Reductase ◽  
Cytochrome P450 Enzymes ◽  
P450 Reductase ◽  
Human Cytochrome ◽  
Redox Potentiometry ◽  
Electron Donation

Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiometry. These studies and the mechanism of electron transfer in CPR are reported herein.

Characterization of human cytochrome P450 enzymes involved in the biotransformation of eperisone

Xenobiotica ◽  
2009 ◽  
Vol 39 (1) ◽  
pp. 1-10 ◽  
Author(s):  
H. H. Yoo ◽  
N. S. Kim ◽  
J. Lee ◽  
D. R. Sohn ◽  
C. Jin ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome

Characterization of Human Cytochrome P450 Enzymes Involved in the Metabolism of Cilostazol

2007 ◽  
Vol 35 (10) ◽  
pp. 1730-1732 ◽  
Author(s):  
Masahiro Hiratsuka ◽  
Yudai Hinai ◽  
Takamitsu Sasaki ◽  
Yumiko Konno ◽  
Kenichi Imagawa ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Enzymes ◽  
Human Cytochrome
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