Double and Triple Helices of Nucleic Acid Polymers, Studied by UV-Resonance Raman Spectroscopy

1993 ◽  
Vol 97 (2) ◽  
pp. 155-162 ◽  
Author(s):  
A. Gfrörer ◽  
M. E. Schnetter ◽  
J. Wolfrum ◽  
K. O. Greulich
Keyword(s):  
Raman Spectroscopy ◽  
Nucleic Acid ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy ◽  
Triple Helices

Structural Transitions and pH-Dependent Equilibria between Unusual Double and Triple Helical Conformations of Nucleic Acid Polymers, Studied by UV Resonance Raman Spectroscopy

1993 ◽  
Vol 47 (7) ◽  
pp. 1064-1070 ◽  
Author(s):  
A. Gfrörer ◽  
M. E. Schnetter ◽  
J. Wolfrum ◽  
K. O. Greulich
Keyword(s):  
Raman Spectroscopy ◽  
Nucleic Acid ◽  
Triple Helix ◽  
Ph Value ◽  
Double Helix ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Excitation Spectra ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy

In order to study pH-driven associations and equilibria between nucleic acid helices containing the homopolymers poly(A) and poly(U), the pH-value of solutions of poly(A) or poly(A)*poly(U) or the triple helix poly(U)*poly(A)*poly(U) was varied in steps of 0.5 pH units between pH = 7.0 and pH = 2.5. Resonance Raman spectra were taken with 260-nm and 220-nm excitation, both in H2O and in D2O solvent. For the solutions containing poly(A) only, marked spectral changes between pH = 6.0 and pH = 5.5 can be attributed to the formation of the parallel stranded double helix poly(A)+*poly(A)+. The cooperativity of this duplex formation is better represented in the 260-nm than in the 220-nm excitation spectra. The double helix poly(A)*poly(U) remains stable down to pH = 5.0. At pH = 4.5 and 4.0, the experimental spectra can be understood by means of a superposition of the spectra of the triple helix poly(U)*poly(A)*poly(U) and the protonated double helix poly(A)+*poly(A)+. The triple helix itself is stable at these pH values, as can be concluded from the spectra of solutions of this polymer. Below pH = 4.0, the spectra can be calculated by the sum of poly(A)+*poly(A)+ and the single-stranded poly(U) polymer. All the helical transitions observed are better represented in the spectra excited with 260 nm, whereas the protonation state is better resolved in the 220-nm spectra. The data reported here provide a basis for further studies of unusual nucleic acid structures as triple and quadruple helices and they show that UV-resonance Raman spectroscopy is a sensitive tool for studying the kinetics and dynamics of the formation of these structures.

The application of UV resonance Raman spectroscopy for the differentiation of clinically relevant Candida species

2018 ◽  
Vol 410 (23) ◽  
pp. 5839-5847 ◽  
Author(s):  
Anja Silge ◽  
Ralf Heinke ◽  
Thomas Bocklitz ◽  
Cornelia Wiegand ◽  
Uta-Christina Hipler ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Candida Species ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Uv Resonance Raman ◽  
Uv Resonance Raman Spectroscopy
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