Biodiesel production using Candida rugosa as biocatalytic lipase immobilized on p ‐nitrobenzyl cellulose xanthate ( NBXCel )

2021 ◽  
Author(s):  
Rafael C. Rial ◽  
Osmar N. Freitas ◽  
Leandro F. Cavalheiro ◽  
Carlos Eduardo D. Nazário ◽  
Luíz Henrique Viana
Keyword(s):  
Candida Rugosa ◽  
Cellulose Xanthate ◽  
Biodiesel Production

scholarly journals Using lipase Candida rugosa as a catalyst for the biodiesel production from Tra fish (Pangasius hypophtalmus) oil

2015 ◽  
Vol 18 (1) ◽  
pp. 29-39
Author(s):  
Nhu Thi Tuyet Nguyen ◽  
Nguyen Thi Nguyen ◽  
Hoa Ngoc Phan
Keyword(s):  
Fatty Acid ◽  
Fish Oil ◽  
Specific Activity ◽  
Candida Rugosa ◽  
Free Water ◽  
Acid Value ◽  
Biodiesel Production ◽  
Molar Ratio ◽  
Buffer Solution ◽  
Solution Ph

In this work, lipase from Candida rugosa (LCR) was used as a catalyst for the transesterification reaction of fish oil with methanol. The research process consists of three stages: determine the material properties and the activity of the enzyme from Candida rugosa, transeter of fish oil with methanol catalyzed by the enzyme lipase, evaluation indicators of the quality of biodiesel obtained. Fish oil contains 62% unsaturated fatty acid, acid value of 2.2 mg KOH/g. Activity and specific activity of enzyme were respectively 1064 U/mg enzyme and 2782 U/mg protein. Factors affecting the efficiency of conversion of fatty acid methyl esters - FAME were investigated: the molar ratio of methanol/fish oil, ratio of enzyme/fish oil, temperature reaction, pH reaction, concentration of buffer and reaction time. Yield of biodiesel conversion was 92.65% with optimal conditions: rate of methanol/fish oil was 4:1, the ratio of enzyme/substract was 2%, reaction temperature was 40°C, additional 10% buffer solution pH 7 with 96 hour response time. Products biodiesel obtained FAME components accounted for 98.94%; density at 15°C is 0.8816 g/ml; no free water and glycerine, consistent with the original standard biodiesel (B100) (TCVN 7717:2007). However, the acid value of 1.7 mg KOH products/g higher than the allowable value.

scholarly journals Co-Immobilization of Rhizopus oryzae and Candida rugosa Lipases onto mMWCNTs@4-arm-PEG-NH2—A Novel Magnetic Nanotube–Polyethylene Glycol Amine Composite—And Its Applications for Biodiesel Production

2021 ◽  
Vol 22 (21) ◽  
pp. 11956
Author(s):  
Saadiah A. Abdulmalek ◽  
Kai Li ◽  
Jianhua Wang ◽  
Michael Kidane Ghide ◽  
Yunjun Yan
Keyword(s):  
Polyethylene Glycol ◽  
Rhizopus Oryzae ◽  
Specific Activity ◽  
Candida Rugosa ◽  
Enzymatic Reaction ◽  
Industrial Applications ◽  
Biodiesel Production ◽  
Synergy Effect ◽  
Immobilized Lipases ◽  
Ultrasound Assisted

This article describes the successful synthesis of a novel nanocomposite of superparamagnetic multi-walled nanotubes with a four-arm polyethylene glycol amine polymer (mMWCNTs@4-arm-PEG-NH2). This composite was then employed as a support for the covalent co-immobilization of Rhizopus oryzae and Candida rugosa lipases under appropriate conditions. The co-immobilized lipases (CIL-mMWCNTs@4-arm-PEG-NH2) exhibited maximum specific activity of 99.626U/mg protein, which was 34.5-fold superior to that of free ROL, and its thermal stability was greatly improved. Most significantly, CIL-mMWCNTs@4-arm-PEG-NH2 was used to prepare biodiesel from waste cooking oil under ultrasound conditions, and within 120 min, the biodiesel conversion rate reached 97.64%. This was due to the synergy effect between ROL and CRL and the ultrasound-assisted enzymatic process, resulting in an increased biodiesel yield in a short reaction time. Moreover, after ten reuse cycles, the co-immobilized lipases still retained a biodiesel yield of over 78.55%, exhibiting excellent operational stability that is attractive for practical applications. Consequently, the combined use of a novel designed carrier, the co-immobilized lipases with synergy effect, and the ultrasound-assisted enzymatic reaction exhibited potential prospects for future applications in biodiesel production and various industrial applications.

Candida rugosa lipase for the biodiesel production from renewable sources

2020 ◽  
Vol 162 ◽  
pp. 124-133
Author(s):  
Mariagrazia Iuliano ◽  
Maria Sarno ◽  
Salvatore De Pasquale ◽  
Eleonora Ponticorvo
Keyword(s):  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Biodiesel Production ◽  
Renewable Sources

scholarly journals Candida rugosa lipase nanoparticles as robust catalyst for biodiesel production in organic solvents

2019 ◽  
Vol 6 (3) ◽  
pp. 1025-1038 ◽  
Author(s):  
Rohit Kumar Sharma ◽  
Crystal A. O'Neill ◽  
Hector A.R. Ramos ◽  
Bibek Thapa ◽  
Vanessa C. Barcelo-Bovea ◽  
...  
Keyword(s):  
Organic Solvents ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Biodiesel Production

Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

2013 ◽  
Vol 30 (6) ◽  
pp. 1335-1338 ◽  
Author(s):  
Jong Ho Lee ◽  
Sung Bong Kim ◽  
Hah Young Yoo ◽  
Ja Hyun Lee ◽  
Sung Ok Han ◽  
...  
Keyword(s):  
Candida Rugosa ◽  
Biodiesel Production

Enzymatic Hydrolysis of Used-Frying Oil Using Candida rugosa Lipase

2014 ◽  
Vol 67 (3) ◽  
Author(s):  
Roslina Rashid ◽  
Nor Athirah Zaharudin ◽  
Ani Idris
Keyword(s):  
Fatty Acids ◽  
Immobilized Lipase ◽  
Candida Rugosa ◽  
Frying Oil ◽  
Biodiesel Production ◽  
Human Consumption ◽  
Free System ◽  
Used Frying Oil ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Hydrolysis of used-frying oil had been carried out by using an immobilized lipase from Candida rugosa in solvent-free system. Used-frying oil was considered as the substrate in this study due to abundance amount of used-frying oil present in Malaysia as its disposal problem has become a very serious environmental issue.  The high free fatty acids (FFA) content in used-frying oil has raised the interest for the utilization of this waste into valuable products. Even though used-frying oil is not suitable for human consumption and being extensively used for the biodiesel production, FFA from used-frying oil could be utilized to produce various types of non-edible products. Effects of enzyme loading, water content, reaction temperature, buffer pH and agitation speeds on the hydrolysis process were investigated. The experiments were conducted at constant reaction time of 3 hours. It was found that the effect of variables were very significant on the hydrolysis process.  The hydrolysis process achieved the highest yield of fatty acids at enzyme concentration of 1.5% (w/v), buffer volume to oil volume ratio of 3:1, temperature of 40˚C, pH of 7, and agitation speed of 220 rpm. Under these described conditions, it was found that nearly 97.15±1.31% of hydrolysis degree was achieved with 2533.33±26.67 µmol/ml of fatty acids was produced.

scholarly journals Interesterifikasi minyak kelapa sawit dengan metil asetat untuk sintesis biodiesel menggunakan candida rugosa lipase terimobilisasi

2018 ◽  
Vol 8 (1) ◽  
pp. 24 ◽  
Author(s):  
Heri Hermansyah ◽  
Septian Marno ◽  
Rita Arbianti ◽  
Tania Surya Utami ◽  
Anondho Wijanarko
Keyword(s):  
Palm Oil ◽  
Methyl Acetate ◽  
Batch Reactor ◽  
Candida Rugosa ◽  
Candida Rugosa Lipase ◽  
Biodiesel Production ◽  
Reactant Ratio ◽  
Adsorption Method ◽  
Promising Alternative ◽  
Biodiesel Synthesis

Palm oil interesterification with methyl acetate for biodiesel synthesis using immobilized Candida rugosa lipaseBiocatalyst is a promising alternative catalyst for synthetic biodiesel because it has capability to improve conventional catalyst weakness, such as product purification and undesired side products. However, biocatalyst is easy to be deactivated by alcohol. Therefore, in this research, new method is developed to maintain the activity and stability of biocatalyst during reaction. In this paper, the experimental results of non-alcohol route synthesis of biodiesel using immobilized candida rugosa lipase in zeolit through adsorption method were reported. Methyl acetate as alkyl acceptor was reacted with triglyceride from palm oil in batch reactor. The analytical results from HPLC showed that trioleat convert up to 82% under the condition of 4%-wt substrate of the biocatalyst concentration and oil/alkyl mole ratio equal to 1/12 in 50 hour reaction. The effects of reactant ratio, biocatalyst concentration on concentration profile of tri-, di-, mono-gliceryde, and biodiesel were also observed. Stability test indicated that the activity of the immobilized biocatalyst still remained active for three reaction cycles.  Michaelis-Menten mechanism was used for derivation kinetic reaction equation to describe the behaviour of biodiesel production. Keywords: Biodiesel, interesterification, Candida rugosa lipase, non-alcohol route, immobilized. AbstrakSaat ini riset sintesis biodiesel menggunakan biokatalis sangat menjanjikan karena mampu memperbaiki kelemahan katalis alkali, yaitu kemudahan pemisahan produk dan kemampuan dalam mengarahkan reaksi secara spesifik tanpa adanya reaksi samping yang tidak diinginkan. Namun, biokatalis mudah terdeaktivasi dalam lingkungan beralkohol. Oleh karena itu, dalam riset ini diusulkan untuk melakukan sintesis biodiesel melalui rute non-alkohol untuk menjaga agar aktivitas dan stabilitas biokatalis tetap tinggi selama reaksi berlangsung. Dalam makalah ini akan disajikan hasil penelitian sintesis biodiesel rute non-alkohol menggunakan Candida rugosa lipase yang diimobilisasi dalam zeolit melalui metode adsorpsi dengan mereaksikan metil asetat sebagai penyuplai gugus alkil dengan trigliserida dari minyak kelapa sawit dalam reaktor batch. Hasil analisis HPLC menunjukkan bahwa lebih dari 82% rantai asam lemak dari trigliserida minyak kelapa sawit berhasil dikonversikan menjadi biodiesel pada kondisi konsentrasi biokatalis sebesar 4%-wt substrat dan rasio mol minyak/alkil sebesar 1/12 selama 50 jam reaksi. Pengaruh rasio reaktan, konsentrasi biokatalis terhadap profil konsentrasi dari tri-, di-, mono-gliserida serta biodiesel juga diselidiki. Uji stabilitas menunjukkan bahwa biokatalis terimobilisasi ini masih memiliki aktivitas untuk tiga kali siklus reaksi. Mekanisme Michaelis-Menten digunakan untuk menurunkan persamaan kinetika reaksi yang mampu menggambarkan perilaku produksi biodiesel yang dihasilkan.Kata kunci: biodiesel, interesterifikasi, Candida rugosa lipase, rute non alkohol, imobilisasi

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