Industrial yeasts strains for biorefinery solutions: Constructing and selecting efficient barcoded xylose fermenting strains for ethanol

2014 ◽  
Vol 8 (5) ◽  
pp. 626-634 ◽  
Author(s):  
Elia Tomás-Pejó ◽  
Nicklas Bonander ◽  
Lisbeth Olsson
Keyword(s):  
Industrial Yeasts

scholarly journals RTM1: a member of a new family of telomeric repeated genes in yeast.

Genetics ◽  
1995 ◽  
Vol 140 (3) ◽  
pp. 945-956
Author(s):  
F Ness ◽  
M Aigle
Keyword(s):  
Ethanol Production ◽  
Laboratory Strain ◽  
Genomic Rearrangement ◽  
Yeast Gene ◽  
New Family ◽  
Nonessential Gene ◽  
Industrial Strains ◽  
Multiple Copies ◽  
New Yeast ◽  
Industrial Yeasts

Abstract We have isolated a new yeast gene called RTM1 whose overexpression confers resistance to the toxicity of molasses. The RTM1 gene encodes a hydrophobic 34-kD protein that contains seven potential transmembrane-spanning segments. Analysis of a series of industrial strains shows that the sequence is present in multiple copies and in variable locations in the genome. RTM loci are always physically associated with SUC telomeric loci. The SUC-RTM sequences are located between X and Y' subtelomeric sequences at chromosome ends. Surprisingly RTM sequences are not detected in the laboratory strain X2180. The lack of this sequence is associated with the absence of any SUC telomeric gene previously described. This observation raises the question of the origin of this nonessential gene. The particular subtelomeric position might explain the SUC-RTM sequence amplification observed in the genome of yeasts used in industrial biomass or ethanol production with molasses as substrate. This SUC-RTM sequence dispersion seems to be a good example of genomic rearrangement playing a role in evolution and environmental adaptation in these industrial yeasts.

Evaluation of industrial yeasts for pathogenicity

1979 ◽  
Vol 17 (1) ◽  
pp. 71-78 ◽  
Author(s):  
D.L. Holzschu ◽  
F.W. Chandler ◽  
L. Ajello ◽  
D.G. Ahearn
Keyword(s):  
Industrial Yeasts

The use of mitochondrial mutants in hybridization of industrial yeasts

1981 ◽  
Vol 4 (3) ◽  
pp. 177-180 ◽  
Author(s):  
J. F. T. Spencer ◽  
Dorothy M. Spencer
Keyword(s):  
Industrial Yeasts

Proteases and caspase-like activity in the yeast Saccharomyces cerevisiae

2011 ◽  
Vol 39 (5) ◽  
pp. 1502-1508 ◽  
Author(s):  
Derek Wilkinson ◽  
Mark Ramsdale
Keyword(s):  
Cell Death ◽  
Cell Fate ◽  
Caspase 3 ◽  
Antifungal Drugs ◽  
Loss Of Function ◽  
Yeast Saccharomyces Cerevisiae ◽  
Cell Death Response ◽  
Substrate Choice ◽  
Industrial Yeasts ◽  
Stimulation Of

A variety of proteases have been implicated in yeast PCD (programmed cell death) including the metacaspase Mca1 and the separase Esp1, the HtrA-like serine protease Nma111, the cathepsin-like serine carboxypeptideases and a range of vacuolar proteases. Proteasomal activity is also shown to have an important role in determining cell fate, with both pro- and anti-apoptotic roles. Caspase 3-, 6- and 8-like activities are detected upon stimulation of yeast PCD, but not all of this activity is associated with Mca1, implicating other proteases with caspase-like activity in the yeast cell death response. Global proteolytic events that accompany PCD are discussed alongside a consideration of the conservation of the death-related degradome (both at the level of substrate choice and cleavage site). The importance of both gain-of-function changes in the degradome as well as loss-of-function changes are highlighted. Better understanding of both death-related proteases and their substrates may facilitate the design of future antifungal drugs or the manipulation of industrial yeasts for commercial exploitation.

scholarly journals How to characterize a strain? The neglected influence of clonal heterogeneity on the phenotypes of industrial Saccharomyces

2020 ◽  
Author(s):  
Hanna Viktória Rácz ◽  
Fezan Mukhtar ◽  
Alexandra Imre ◽  
Zoltán Rádai ◽  
Andreas Károly Gombert ◽  
...  
Keyword(s):  
Single Cell ◽  
Yeast Strain ◽  
Genome Structure ◽  
Clonal Lineage ◽  
Industrial Yeast ◽  
Clonal Interference ◽  
Clonal Heterogeneity ◽  
Colony Area ◽  
Industrial Yeast Strain ◽  
Industrial Yeasts

AbstractPopulations of microbes are constantly evolving heterogeneity that selection acts upon, yet heterogeneity is non-trivial to assess methodologically. The practice of isolating single cell colonies for establishing, transferring, and using a strain results in single-cell bottlenecks with a generally neglected effect on the characteristics of the strain. We used six industrial yeasts to assess the level of heterogeneity in clonal populations, especially in terms of stress tolerance. First, we uncovered the existence of genome structure variants in available sequenced genomes of clonal lineages of thes strains. Subsequent phenotyping of strains and their newly isolated subclones showed that single-cell bottlenecks during isolation can considerably influence the observable phenotype. Next, we decoupled fitness distributions on the level of individual cells from clonal interference by plating single cell colonies. We used the obtained data on colony area for statistical modeling of the heterogeneity in phenotypes. One strain was further used to show how individual subclonal lineages are remarkably different not just in phenotype, but also in the level of heterogeneity. Thereby we call attention to the fact that choosing an initial clonal lineage from an industrial yeast strain may vastly influence downstream performances and observations on geno- and phenotype, and also on heterogeneity.

Magnesium, Calcium, and Fermentative Metabolism in Industrial Yeasts

1996 ◽  
Vol 54 (1) ◽  
pp. 13-18 ◽  
Author(s):  
G. M. Walker ◽  
R. M. Birch ◽  
G. Chandrasena ◽  
A. I. Maynard
Keyword(s):  
Fermentative Metabolism ◽  
Industrial Yeasts
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