A convenient homogeneous enzyme immunoassay for estradiol detection

10.1002/bab.5 ◽  
2011 ◽  
Vol 58 (1) ◽  
pp. 75-82 ◽  
Author(s):  
May L. Chiu ◽  
Tina T.-C. Tseng ◽  
Harold G. Monbouquette
Keyword(s):  
Enzyme Immunoassay ◽  
Homogeneous Enzyme Immunoassay

Principles of Homogeneous Enzyme-Immunoassay

2018 ◽  
pp. 105-134
Author(s):  
Edwin F. Ullman ◽  
Edward T. Maggio
Keyword(s):  
Enzyme Immunoassay ◽  
Homogeneous Enzyme Immunoassay

Homogeneous enzyme immunoassay for tobramycin evaluated and compared with a radioimmunoassay.

1982 ◽  
Vol 28 (6) ◽  
pp. 1370-1374 ◽  
Author(s):  
J Woo ◽  
M A Longley ◽  
D C Cannon
Keyword(s):  
Enzyme Immunoassay ◽  
Room Temperature ◽  
Correlation Study ◽  
Stability Study ◽  
Data Handling ◽  
Handling System ◽  
Analytical Recovery ◽  
Assay Precision ◽  
Absorbance Data ◽  
Homogeneous Enzyme Immunoassay

Abstract We evaluated a commercially available homogeneous enzyme immunoassay (EMIT, Syva Co.) for tobramycin against a reference radioimmunoassay (RIA) method. Between-assay precision (CV) was 2.9% at 6.2 mg/L and 3.0% for values in the range of 1.0-7.6 mg/L. Accuracy based on a recovery experiment (1.0-13.0 mg/L) yielded an analytical recovery of 88-112%. A correlation study with 75 sera from patients on tobramycin therapy showed that EMIT = 0.984 RIA - 0.0808, r = 0.993. Neither the EMIT nor the RIA procedure was affected by the presence of gentamicin, amikacin, and vancomycin. Absorbance data from the EMIT system calculated with the conventional RIA logit-log algorithm correlate well with results generated by the Syva data-handling system (logit-log = 1.077 Syva - 0.318, r = 0.998). A reagent stability study indicated that the EMIT reagents, once reconstituted, remain stable for at least 17 days when stored at refrigerated temperatures, or 11 days if stored at room temperature, thus enabling frequent "stat" assays without the need to prepare a calibration curve each time.

Evaluation of homogeneous enzyme immunoassay of serum thyroxine with the Gilford 3500 analyzer.

1979 ◽  
Vol 25 (8) ◽  
pp. 1469-1471 ◽  
Author(s):  
K L Cruse
Keyword(s):  
Enzyme Immunoassay ◽  
Serum Thyroxine ◽  
Precision And Accuracy ◽  
Homogeneous Enzyme Immunoassay

Abstract I evaluated homogeneous enzyme immunoassay (EMIT) of serum thyroxine with the Gilford 3500 analyzer. The samples are pretreated before quantitation on the Gilford 3500; the procedure thereafter is entirely mechanized. Forty-four samples may be analyzed per run. Precision and accuracy are good, and results correlate well with those by radioimmunoassay.

A New Homogeneous Enzyme Immunoassay. Its Application to Measurement of α-Fetoprotein

1985 ◽  
Vol 97 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Nobuhiro HOSHINO ◽  
Michio HAMA ◽  
Rie SUZUKI ◽  
Yuko KATAOKA ◽  
Gilbu SOE
Keyword(s):  
Enzyme Immunoassay ◽  
Homogeneous Enzyme Immunoassay

scholarly journals Homogeneous Enzyme Immunoassay for Triiodothyronine in Serum

2001 ◽  
Vol 47 (3) ◽  
pp. 569-574 ◽  
Author(s):  
Christina D Karapitta ◽  
Theodore G Sotiroudis ◽  
Athanassios Papadimitriou ◽  
Aristotelis Xenakis
Keyword(s):  
Enzyme Immunoassay ◽  
Glycogen Phosphorylase ◽  
Serum Proteins ◽  
Rabbit Muscle ◽  
Glycogen Phosphorylase B ◽  
Low Concentrations ◽  
Enzyme Conjugate ◽  
High Concentrations ◽  
Treatment Step ◽  
Homogeneous Enzyme Immunoassay

Abstract Background: The concentration of triiodothyronine (T3) in human serum is extremely low and can be determined only by very sensitive methods. We developed a homogeneous enzyme immunoassay for T3 analysis in unextracted serum. Methods: A T3 derivative was conjugated to the −SH groups of glycogen phosphorylase b (GPb) from rabbit muscle. Conjugation caused inhibition of enzyme activity, and the enzyme conjugate was reactivated upon binding of anti-T3 antibody. Activation was blocked by the presence of non-antibody-bound T3; this was the basis for the development of the homogeneous enzyme immunoassay for T3 by determining GPb activity fluorometrically. Results: We used furosemide to block the interaction of T3 with serum proteins with T3-binding sites, avoiding any serum treatment step. T3 was measured in the range 0.3–8 μg/L. T3 values obtained by this assay correlated well with those obtained by a RIA (y = 0.97x − 0.07 μg/L; r = 0.96; n = 92). Within- and between-run imprecision (CV) was 5–9% for normal and high concentrations and 16–20% for low concentrations. Conclusions: Chemical modification of GPb with a T3 derivative allows the development of a simple homogeneous enzyme immunoassay for T3 in unextracted serum.

Homogeneous enzyme immunoassay for cortisol with a centrifugal analyzer.

1984 ◽  
Vol 30 (11) ◽  
pp. 1824-1826 ◽  
Author(s):  
J M Izquierdo ◽  
A Quirós ◽  
J Alvarez-Uría ◽  
P Sotorrío
Keyword(s):  
Enzyme Immunoassay ◽  
Protein Complex ◽  
Acidic Solution ◽  
Serum Cortisol ◽  
Standard Curve ◽  
Analytical Recovery ◽  
Target Values ◽  
Assay Precision ◽  
Homogeneous Enzyme Immunoassay ◽  
Control Samples

Abstract We determine serum cortisol by a homogeneous enzyme immunoassay in the Cobas Bio centrifugal analyzer. To unbind cortisol from its protein complex, serum is treated for 15 min with an acidic solution. The reaction then proceeds automatically in the analyzer at 37 degrees C. To 50 microL of sample mixture is added 125 microL of reagent (cortisol antibodies, glucose 6-phosphate, and NAD+). This mixture is incubated for 60 s, after which 25 microL of a cortisol derivative labeled with glucose 6-phosphate is added; the increase in absorbance is monitored at 340 nm. The standard curve was linear from 10 to 500 micrograms of cortisol per liter. Within-assay precision (CV) varied from 0.2 to 0.6%, between-assay precision from 6.2 to 10.6%. Analytical recovery ranged from 100 to 103%. Results for control samples deviated from target values by 1.4 to 7.8%. Results compared well with those by radioimmunoassay. The method is reliable and practicable and will usefully replace previous routine methods for serum cortisol.

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