scholarly journals Development of a Recombinant Nucleocapsid Protein‐Based ELISA for Detection of IgM and IgG Antibodies to SARS‐CoV‐2

2021 ◽  
Author(s):  
Maryam Ranjbar ◽  
Marzieh Asadi ◽  
Marjan Nourigorji ◽  
Bahador Sarkari ◽  
Zohreh Mostafavi‐Pour ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Igg Antibodies ◽  
Recombinant Nucleocapsid Protein

scholarly journals Serological Survey of Hantavirus in Inhabitants from Tropical and Subtropical Areas of Brazil

2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
Felipe Alves Morais ◽  
Alexandre Pereira ◽  
Aparecida Santo Pietro Pereira ◽  
Marcos Lazaro Moreli ◽  
Luís Marcelo Aranha Camargo ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Atlantic Rain Forest ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Tropical Area ◽  
Forest Region ◽  
Human Sera ◽  
Tropical Areas ◽  
Antibody Levels ◽  
Recombinant Nucleocapsid Protein

Brazil has reported more than 1,600 cases of hantavirus cardiopulmonary syndrome (HPS) since 1993, with a 39% rate of reported fatalities. Using a recombinant nucleocapsid protein ofAraraquaravirus, we performed ELISA to detect IgG antibodies against hantavirus in human sera. The aim of this study was to analyze hantavirus antibody levels in inhabitants from a tropical area (Amazon region) in Rondônia state and a subtropical (Atlantic Rain Forest) region in São Paulo state, Brazil. A total of 1,310 serum samples were obtained between 2003 and 2008 and tested by IgG-ELISA, and 82 samples (6.2%), of which 62 were from the tropical area (5.8%) and 20 from the subtropical area (8.3%), tested positive. Higher levels of hantavirus antibody were observed in inhabitants of the populous subtropical areas compared with those from the tropical areas in Brazil.

scholarly journals Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ariel Munitz ◽  
L. Edry-Botzer ◽  
M. Itan ◽  
R. Tur-Kaspa ◽  
D. Dicker ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Neutralizing Antibodies ◽  
Host Response ◽  
Humoral Response ◽  
Rapid Onset ◽  
Response Analysis ◽  
Receptor Binding Domain ◽  
Igg Antibodies ◽  
Viral Receptor ◽  
Iga Antibodies

AbstractDespite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding the magnitude and duration of the humoral response. Analysis of the antibody response in mild versus moderate/severe patients, using our new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies toward SARS-CoV-2 antigens, revealed a rapid onset of IgG/IgA antibodies, specifically in moderate/severe patients. IgM antibodies against the viral receptor binding domain, but not against nucleocapsid protein, were detected at early stages of the disease. Furthermore, we observed a marked reduction in IgM/IgA antibodies over-time. Adapting our assay for ACE2 binding-competition, demonstrated that the presence of potentially neutralizing antibodies is corelated with IgG/IgA. Finally, analysis of the cytokine profile in COVID-19 patients revealed unique correlation of an IL-12p70/IL33 and IgG seroconversion, which correlated with disease severity. In summary, our comprehensive analysis has major implications on the understanding and monitoring of SARS-CoV-2 infections.

scholarly journals Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2

Virology ◽  
2021 ◽  
Vol 557 ◽  
pp. 15-22
Author(s):  
Teodora Djukic ◽  
Maja Mladenovic ◽  
Dragana Stanic-Vucinic ◽  
Jelena Radosavljevic ◽  
Katarina Smiljanic ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Protein Fragment ◽  
Immunological Characterization ◽  
Recombinant Nucleocapsid Protein

scholarly journals Recombinant nucleocapsid protein-based IgG enzyme-linked immunosorbent assay for the serological diagnosis of SARS

2005 ◽  
Vol 125 (2) ◽  
pp. 181-186 ◽  
Author(s):  
Masayuki Saijo ◽  
Toshio Ogino ◽  
Fumihiro Taguchi ◽  
Shuetsu Fukushi ◽  
Tetsuya Mizutani ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Nucleocapsid Protein ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Nucleocapsid Protein

Production of a polyclonal antiserum against recombinant nucleocapsid protein and its application for the detection of fig mosaic virus

2019 ◽  
Vol 265 ◽  
pp. 22-25 ◽  
Author(s):  
Morteza Shahmirzaie ◽  
Mohammad Reza Safarnejad ◽  
Farshad Rakhshandehroo ◽  
Hossein Safarpour ◽  
Hodjattallah Rabbani ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Nucleocapsid Protein ◽  
Polyclonal Antiserum ◽  
Fig Mosaic Virus ◽  
Recombinant Nucleocapsid Protein
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