Analysis of the effects of different nitrogen sources and calcium on the production of amphotericin by Streptomyces nodosus based on comparative transcriptome

2021 ◽  
Author(s):  
Kai Huang ◽  
Bo Zhang ◽  
Yu Chen ◽  
Zhe‐Ming Wu ◽  
Zhi‐Qiang Liu ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
Comparative Transcriptome ◽  
Streptomyces Nodosus

scholarly journals Comparative Transcriptome Analysis of Streptomyces nodosus Mutant With a High-Yield Amphotericin B

2021 ◽  
Vol 8 ◽  
Author(s):  
Kai Huang ◽  
Bo Zhang ◽  
Yu Chen ◽  
Zhi-Qiang Liu ◽  
Yu-Guo Zheng
Keyword(s):  
Secondary Metabolites ◽  
Gene Cluster ◽  
Amphotericin B ◽  
Pentose Phosphate ◽  
Phosphoglycerate Mutase ◽  
High Yield ◽  
Original Strain ◽  
Comparative Transcriptome ◽  
Polyene Macrolide Antibiotic ◽  
Streptomyces Nodosus

Antibiotics play an important role in human health. Most antibiotics are derived from microbial secondary metabolites. Amphotericin is a polyene macrolide antibiotic synthesized by Streptomyces nodosus. S. nodosus ZJB2016050 with high-yield amphotericin B (AmB) was obtained by traditional mutagenesis using S. nodosus ATCC14899 as the original strain. The differences in the characterization of the two strains were found in color, mycelium morphology, and AmB yield. Subsequent comparative transcriptome explained the yield differences between the two strains. Pathways including the carbohydrate metabolic pathway and the secondary product synthesis pathway were targeted. The upregulation of glucokinase, phosphoglycerate mutase, and pyruvate dehydrogenase accelerates the consumption of glucose and has great effects on the accumulation of precursors. One of the competitive secondary metabolites of the polyketone synthetase (PKS) II type sapromomycin analog synthesis gene cluster was downregulated, which competes for malonyl-CoA. Five PKS modules (except for the first module amphA) of the amphotericin synthetic gene cluster in the high-yielding strain were downregulated, which resulted in the total amphotericin A (AmA) and AmB of S. nodosus ZJB2016050 being less than that of the wild-type S. nodosus ATCC14899. Combined with gene differential expression in the pentose phosphate pathway and the reaction mechanism of the ER5 domain, the reason that S. nodosus ZJB2016050 preferred to synthesize AmB was probably related to intracellular reduction.

Linking nitrogen sources utilised by seagrass in a temperate marine embayment to patterns of seagrass change during drought

2016 ◽  
Vol 549 ◽  
pp. 79-88 ◽  
Author(s):  
AJ Hirst ◽  
AR Longmore ◽  
D Ball ◽  
PLM Cook ◽  
GP Jenkins
Keyword(s):  
Nitrogen Sources ◽  
Marine Embayment

Effect of Nitrogen Sources on the AZO Dye Decolourization

2012 ◽  
Vol 2 (7) ◽  
pp. 424-426
Author(s):  
Suchita Dahiwade ◽  
◽  
Dr. A. O. Ingle Dr. A. O. Ingle ◽  
Dr. S. R. Wate Dr. S. R. Wate
Keyword(s):  
Azo Dye ◽  
Nitrogen Sources ◽  
Dye Decolourization

IDENTIFICATION AND CHARACTERIZATION OF A PURPLE NONSULFUR BACTERIUM ISOLATED FROM COASTAL AREA OF HAI PHONG FOR USING IN PRODUCTION OF UNSATURATED FATTY ACID (OMEGA 6, 7, 9)

2019 ◽  
Vol 57 (6) ◽  
pp. 665 ◽  
Author(s):  
Yen Thi Hoang ◽  
Quynh Thi Thu Tran ◽  
Ha Hoang Chu ◽  
Tuyen Thi Do ◽  
Thanh Tat Dang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Unsaturated Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Nitrogen Sources ◽  
16S Rrna Analysis ◽  
Rhodovulum Sulfidophilum ◽  
Purple Nonsulfur Bacterium ◽  
High Lipid ◽  
Physiological Properties ◽  
Omega 6

Purple nonsulfur bacteria are a group that has so much biotechnological applications, particularly in producing of functional food rich with unsaturated fatty acids. A purple nonsulfur bacterium (named HPB.6) was chosen based on its strong growth, high lipid and synthesis of unsaturated fatty acid (omega 6,7,9). Studying on basic biological characteristics showed that the cells of HPB.6 were observed as ovoid-rod shape, none motility, Gram negative staining. The diameter of single bacterium was about 0.8-1.0 µm. The cells divide by binary fission and had bacteriochlorophyll a (Bchl a). This bacterium grew well on medium with carbon and nitrogen sources such as acetate, succinate, pyruvate, butyrate, glutamate, arginine, leucine, tyrosine, alanine, methionine, threonine, glutamine, yeast extract and NH4Cl. This selected strain grew well on medium with salt concentrations from 1.5 - 6.0% (optimum 3%), pH from 5.0 to 8.0 (optimum at pH 6.5) and could withstand Na2S at 4.0 - 5.2 mM. Based on morphological, physiological properties and 16S rRNA analysis received demonstrated that HPB.6 strain belongs to the species Rhodovulum sulfidophilum.

Sarcosine and Creatine as Nitrogen Sources for the Ruminant, and the Influence of Supplemental Vitamin B12

1959 ◽  
Vol 18 (2) ◽  
pp. 780-789 ◽  
Author(s):  
C. D. Campbell ◽  
G. A. McLaren ◽  
G. C. Anderson ◽  
J. A. Welch ◽  
G. S. Smith ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Nitrogen Sources ◽  
Supplemental Vitamin
Sign in / Sign up

Export Citation Format

Share Document