Successful use of Nicotiana tabacum hairy roots for the recombinant production of Cecropin A peptide

2021 ◽  
Author(s):  
Samaneh Hashemi ◽  
Ali Niazi ◽  
Amin Baghizadeh ◽  
Mohammad Sadegh Taghizadeh
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Recombinant Production ◽  
Cecropin A

Antioxidant response of tobacco (Nicotiana tabacum) hairy roots after phenol treatment

2011 ◽  
Vol 49 (9) ◽  
pp. 1020-1028 ◽  
Author(s):  
Lucas G. Sosa Alderete ◽  
Elizabeth Agostini ◽  
María I. Medina
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Antioxidant Response ◽  
Phenol Treatment

scholarly journals Enhanced secretion of tropane alkaloids in Nicotiana tabacum hairy roots expressing heterologous hyoscyamine-6β-hydroxylase

2005 ◽  
Vol 56 (420) ◽  
pp. 2611-2618 ◽  
Author(s):  
Suvi T. Häkkinen ◽  
Elisabeth Moyano ◽  
Rosa M. Cusidó ◽  
Javier Palazón ◽  
M. Teresa Piñol ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Tropane Alkaloids ◽  
Enhanced Secretion

scholarly journals Expression of the antibody 14D9 in Nicotiana tabacum hairy roots

2005 ◽  
Vol 8 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Carolina Martinez ◽  
Silvana Petruccelli ◽  
Ana Maria Giulietti ◽  
Maria Alejandra Alvarez
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots

Sesquiterpenoid phytoalexins produced by hairy roots of Nicotiana tabacum

1994 ◽  
Vol 37 (2) ◽  
pp. 349-351 ◽  
Author(s):  
Mark S. Wibberley ◽  
John R. Lenton ◽  
Steven J. Neill
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Roots

Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity

2019 ◽  
Vol 61 (4) ◽  
pp. 241-252 ◽  
Author(s):  
Marzieh Varasteh Shams ◽  
Farhad Nazarian-Firouzabadi ◽  
Ahmad Ismaili ◽  
Reza Shirzadian-Khorramabad
Keyword(s):  
Antimicrobial Activity ◽  
Nicotiana Tabacum ◽  
Hairy Roots

scholarly journals Heterologous production of hyaluronic acid in Nicotiana tabacum hairy roots expressing a human hyaluronan synthase 2

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Arezoo Nazeri ◽  
Ali Niazi ◽  
Alireza Afsharifar ◽  
Seyed Mohsen Taghavi ◽  
Ali Moghadam ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Large Scale ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Maximum Activity ◽  
Bacterial Strains ◽  
Elisa Kit ◽  
Wet Weight

AbstractHyaluronic acid (HA), a unique polysaccharide with excellent Physico-chemical properties, is broadly used in pharmaceutical, biomedical, and cosmetic fields. It is widely present in all vertebrates, certain bacterial strains, and even viruses while it is not found in plants, fungi, and insects. HA is naturally synthesized by a class of integral membrane proteins called Hyaluronic acid synthase (HAS). Thus far, industrial production of HA is carried out based on either extraction from animal sources or large-scale microbial fermentation. The major drawbacks to using these systems are contamination with pathogens and microbial toxins. Recently, the production of HA through recombinant systems has received considerable attention. Plants are eco-friendly ideal expression systems for biopharmaceuticals production. In this study, the optimized human hyaluronic acid synthase2 (hHAS2) sequence was transformed into Nicotiana tabacum using Agrobacterium rhizogenes. The highest rhHAS2 concentration of 65.72 ng/kg (wet weight) in transgenic tobacco hairy roots was measured by the human HAS2 ELISA kit. The HA production in the transgenic hairy roots was verified by scanning electron microscope (SEM) and quantified by the HA ELISA kit. The DPPH radical scavenging activity of HA with the highest concentration of 0.56 g/kg (wet weight) showed a maximum activity of 46%. Gel Permeation Chromatography (GPC) analyses revealed the high molecular weight HA (HMW-HA) with about > 0.8 MDa.

scholarly journals Heterologous production of Hyaluronic Acid in Nicotiana tabacum hairy roots expressing a human Hyaluronan synthase 2

2021 ◽  
Author(s):  
arezoo nazeri ◽  
Ali Niazi ◽  
Alireza Afsharifar ◽  
Seyed Mohsen Taghavi ◽  
Ali Moghadam ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Nicotiana Tabacum ◽  
Hairy Roots ◽  
Large Scale ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gel Permeation Chromatography ◽  
Maximum Activity ◽  
Bacterial Strains ◽  
Wet Weight

Abstract Hyaluronic acid (HA), a unique polysaccharide with excellent physicochemical properties, is broadly used in pharmaceutical, biomedical, and cosmetic fields. It is widely present in all vertebrates, certain bacterial strains, and even viruses while it is not found in plants, fungi, and insects. HA is naturally synthesized by a class of integral membrane proteins called Hyaluronic acid synthase (HAS). Thus far, industrial production of HA is carried out based on either extraction from animal sources or large-scale microbial fermentation. The major drawbacks to using these systems are contamination with pathogens and microbial toxins. Recently, the production of HA through recombinant systems has received considerable attention. Plants are eco-friendly ideal expression systems for biopharmaceuticals production. In this research, the optimized human hyaluronic acid synthase2 (hHAS2) sequence was transformed into Nicotiana tabacum using Agrobacterium rhizogenes. The highest hHAS2 production in the tobacco hairy roots was 65.72 ng/kg (wet weight). The extracted HA was verified and quantified by the HA ELISA kit. The DPPH radical scavenging activity of HA with the highest concentration of 0.56 g/kg (wet weight) showed the maximum activity of 46%. Gel Permeation Chromatography (GPC) analyses revealed the high molecular weight HA with about > 0.8 MDa.

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