Generation of human scFv–IgG1Fc antibodies for detection of lymphatic filarial recombinant antigens, BmR1 and BmSXP

2020 ◽  
Author(s):  
Ahmad Ismail Khaled Abdo ◽  
Ying Yuan Ngoh ◽  
Min Han Lew ◽  
Sylvia Annabel Dass ◽  
Anizah Rahumatullah ◽  
...  
Keyword(s):  
Recombinant Antigens ◽  
Human Scfv

scholarly journals Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zahra Shadman ◽  
Safar Farajnia ◽  
Mohammad Pazhang ◽  
Mohammadreza Tohidkia ◽  
Leila Rahbarnia ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Virulence Factors ◽  
High Reactivity ◽  
Scfv Antibody ◽  
Phage Library ◽  
Human Antibody ◽  
Exotoxin A ◽  
Phage Elisa ◽  
Human Scfv ◽  
Domain I

Abstract Background Pseudomonas aeruginosa is the leading cause of nosocomial infections, especially in people with a compromised immune system. Targeting virulence factors by neutralizing antibodies is a novel paradigm for the treatment of antibiotic-resistant pseudomonas infections. In this respect, exotoxin A is one of the most potent virulence factors in P. aeruginosa. The present study was carried out to identify a novel human scFv antibody against the P. aeruginosa exotoxin A domain I (ExoA-DI) from a human scFv phage library. Methods The recombinant ExoA-DI of P. aeruginosa was expressed in E. coli, purified by Ni-NTA column, and used for screening of human antibody phage library. A novel screening procedure was conducted to prevent the elimination of rare specific clones. The phage clone with high reactivity was evaluated by ELISA and western blot. Results Based on the results of polyclonal phage ELISA, the fifth round of biopanning leads to the isolation of several ExoA-DI reactive clones. One positive clone with high affinity was selected by monoclonal phage ELISA and used for antibody expression. The purified scFv showed high reactivity with the recombinant domain I and full-length native exotoxin A. Conclusions The purified anti-exotoxin A scFv displayed high specificity against exotoxin A. The human scFv identified in this study could be the groundwork for developing a novel therapeutic agent to control P. aeruginosa infections.

scholarly journals Construction of human ScFv phage display library against ovarian tumor

2006 ◽  
Vol 26 (5) ◽  
pp. 497-499 ◽  
Author(s):  
Jinsong Xia ◽  
Hao Bi ◽  
Qin Yao ◽  
Shen Qu ◽  
Yiqiang Zong
Keyword(s):  
Phage Display ◽  
Ovarian Tumor ◽  
Phage Display Library ◽  
Human Scfv

scholarly journals Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis

PLoS ONE ◽  
2017 ◽  
Vol 12 (9) ◽  
pp. e0184867 ◽  
Author(s):  
Franklin B. Magalhães ◽  
Artur L. Castro Neto ◽  
Marilia B. Nascimento ◽  
Wagner J. T. Santos ◽  
Zulma M. Medeiros ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Serological Diagnosis ◽  
Canine Visceral Leishmaniasis ◽  
Recombinant Antigens

Polyvalent recombinant antigens: a new vaccine strategy

Vaccine ◽  
1988 ◽  
Vol 6 (4) ◽  
pp. 304-306 ◽  
Author(s):  
S.M. Kingsman ◽  
A.J. Kingsman
Keyword(s):  
Vaccine Strategy ◽  
Recombinant Antigens

USE OF RECOMBINANT ANTIGENS FOR DETECTION OFTOXOPLASMA GONDIIINFECTION IN SWINE

2000 ◽  
Vol 86 (3) ◽  
pp. 459-462 ◽  
Author(s):  
H. Ray Gamble ◽  
Carolyn D. Andrews ◽  
J. P. Dubey ◽  
Donald W. Webert ◽  
Stephen F. Parmley
Keyword(s):  
Recombinant Antigens
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